ChIP was conducted following a previously-described method [77] (link). Each experiment used 1.5 grams of tissue from 11-day-old seedlings. All ChIP experiments used at least two biological replicates and at least two technical replicates. Anti-H3K4me3 (07-473) and anti-H3K27me3 (ab6002) were purchased from Millipore and Abcam, respectively. IgG Sepharose 6 Fast Flow (GE Healthcare) was used for ChIP using RRP6L1-TAP tagged line. The mock antibody control used an equal amount of chromatin that was not treated with antibody. The ChIPed DNA was purified using PCR purification kit (Fermentas) and qPCR was performed. Supplemental Table S1 lists the primers used for PCR.
Anti h3k4me3 07 473
Manufactured by Merck Group
Anti-H3K4me3 (07-473) is a laboratory reagent used for the detection and analysis of histone H3 trimethylation at lysine 4 (H3K4me3) in biological samples. It is a specific antibody that can be used in various analytical techniques, such as Western blotting, immunoprecipitation, and chromatin immunoprecipitation (ChIP).
Automatically generated - may contain errors
Lab products found in correlation
Pcr purification kit, by Thermo Fisher Scientific (1 mentions)
Igg sepharose 6 fast flow, by GE Healthcare (1 mentions)
Ab23980, by Abcam (1 mentions)
Anti h3k27ac 39133, by Active Motif (1 mentions)
Anti cdk2 sc 163, by Santa Cruz Biotechnology (1 mentions)
Rabbit igg i5006, by Merck Group (1 mentions)
Anti ha ha 11 clone 16b12, by Fortrea (1 mentions)
2 protocols using anti h3k4me3 07 473
1
ChIP Assay for Histone Modifications
2
Antibody Validation for Protein Analysis
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The following antibodies were used: anti-SKI (sc-9140, Santa Cruz); anti-RUNX1 (ab23980, Abcam); anti-CDK2 (sc-163, Santa Cruz); anti-Flag (F3163, Sigma); anti-HA (HA.11 clone 16B12; Covance); anti-H3K4me3 (07-473, Millipore); anti-H3K27ac (39133, Active Motif); rabbit IgG (I5006, Sigma).
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