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H 4835 gmp

Manufactured by Bachem

H-4835-GMP is a laboratory equipment product manufactured by Bachem. It is designed for use in a range of scientific and research applications. The core function of this product is to provide a controlled and stable environment for various experimental processes.

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2 protocols using h 4835 gmp

1

Parathyroid Hormone Peptide Ligand Assay

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The peptide ligands parathyroid hormone PTH (1 (link)34 (link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, no link found, no link found, link, link, link, link, link)) (human, H-4835-GMP, 4033364) and parathyroid hormone-related peptide (PTHrP) (1 (link)34 (link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, link, no link found, no link found, link, link, link, link, link)) (human, mouse, rat; 4017147) were from Bachem. SNAP-Cell 647-SiR (S9102S) was from New England Biosciences. Anti–HA-tag antibody (ab9110) was from Abcam and anti-rabbit IgG, horseradish peroxidase (HRP)-linked antibody (7074P2) was from Cell Signaling. The 3,3′,5,5′-tetramethylbenzidine (TMB, T8665) was from Sigma-Aldrich. NanoBRET Nano-Glo Substrate–furimazine (N1663) and HaloTag NanoBRET 618 Ligand (G9801) were from Promega. Dimethyl sulfoxide (A994.2) for cell culture was from Carl Roth GmbH & Co. KG. Bovine serum albumin (BSA, SAFSA7030) was from VWR International.
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2

Peptide Ligand Binding Assay

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The peptide ligands parathyroid hormone PTH (1-34) (human, H-4835-GMP, #4033364) and parathyroid hormone-related peptide (PTHrP) (1-34) (human, mouse, rat; #4017147) were from Bachem. SNAP-Cell 647-SiR (#S9102S) was from New England Biosciences. Anti HA-tag antibody (ab9110) was from Abcam and anti-rabbit IgG, HRP-linked antibody (#7074P2) was from Cell Signalling. 3,3′,5,5′-Tetramethylbenzidine (TMB, #T8665) was from Sigma-Aldrich. NanoBRET™ Nano-Glo® Substrate -furimazine (#N1663) and HaloTag® NanoBRET™ 618 Ligand (#G9801) were from Promega. Dimethylsulfoxide (DMSO, #A994.2) for cell culture was from Carl Roth GmbH & Co. KG. Bovine Serum Albumin (BSA, #SAFSA7030) was from VWR International.
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