Protein extraction and Western blot analysis for cells were performed as previously described [53 (link)]. Frozen tumor tissues were homogenized and lysed in 5 volumes cold RIPA lysis buffer (Pierce), containing 1x phosphatase (Thermo Scientific) and protease inhibitor cocktail (Roche). Primary antibodies included: phospho-p42/p44 extracellular signal–regulated kinase (ERK) [1:1000, anti-rabbit (Cell Signaling, 4376)], total ERK [1:1000, anti-rabbit (Cell Signaling, 9102)], CDK4 [1:1000, anti-rabbit (Cell signaling, 12790)], CDK6 [1:1000, anti-rabbit (Cell signaling, 12790)], cyclin D1 [1:2000, anti-mouse (Cell Signaling, 2926)], p-S780 Rb [1:500, anti-rabbit (Abcam, ab44763)], total RB [(1:200, anti-rabbit (Santa Cruz, sc-50)], and beta actin [(1:5000, anti-mouse (Sigma, A5441). The protein-antibody complexes were detected by using an enhanced chemiluminescence kit (ThermoFisher Scientific) according to the manufacturer’s recommended protocol.
P s780 rb
Manufactured by Abcam
The P-S780 Rb is a laboratory equipment product. It serves as a core function related to its intended purpose, without further interpretation or extrapolation.
Automatically generated - may contain errors
Lab products found in correlation
Enhanced chemiluminescence kit, by Thermo Fisher Scientific (1 mentions)
Cyclin d1, by Cell Signaling Technology (1 mentions)
Phospho p42 p44, by Cell Signaling Technology (1 mentions)
Total erk, by Cell Signaling Technology (1 mentions)
Protease inhibitor cocktail, by Roche (1 mentions)
Polii, by Abcam (1 mentions)
P s5 polii, by Abcam (1 mentions)
P s2 polii, by Abcam (1 mentions)
P t161 t160 cdk1 cdk2, by Abcam (1 mentions)
Ps15 p53, by Cell Signaling Technology (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
β actin, by Abcam (1 mentions)
Cleaved parp1, by Abcam (1 mentions)
2 protocols using p s780 rb
1
Western Blot Analysis of Tumor Tissues
2
Immunoblotting of Cell Cycle Regulators
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Immunoblotting was carried out as previously described [40 (link)] using the following primary antibodies: CDK7 (Cell Signaling Technology, 2916), CDK1 (Cell Signaling Technology, 9116), CDK2 (Cell Signaling Technology, 2546), CDK4 (Cell Signaling Technology, 12790), CDK9 (Cell Signaling Technology, 2316), β-actin (Abcam, ab6276), GAPDH (Cell Signaling Technology, 2118), P-S5 PolII (Abcam, ab5401), P-S2 PolII (Abcam, ab5095), PolII (Abcam, ab817), P-S780 Rb (Abcam, ab47763), P-S807/811 Rb (Cell Signaling Technology, 8516), Rb (Abcam, ab6075), cyclin H (Abcam, ab54903), MAT1 (Santa Cruz Biotechnology, sc135981), P-S15 p53 (Cell Signaling Technology, 9284), p53 (Santa Cruz Biotechnology, sc-126), P-T161/T160 CDK1/CDK2 (Abcam, ab201008), P-S10 Histone H3 (Abcam, ab139417), AR (MilliporeSigma, 06-680), P-T1457 MED1 (Abcam, ab60950), MED1 (Bethyl Laboratories, A300-793A), cleaved PARP1 (Abcam, ab4830), p21 (Santa Cruz Biotechnology, sc-817).
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