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Ficoll paque plus gradient separation

Manufactured by GE Healthcare
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Ficoll-paque PLUS is a density gradient medium used for the separation and isolation of cells and cellular components from biological samples. It is designed to facilitate the isolation of specific cell types, such as mononuclear cells, from whole blood or other complex mixtures. The product's core function is to enable the efficient and gentle separation of cells based on their density differences when centrifuged.

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Close spelling variants of this product

Ficoll-Paque gradient separation Ficoll-Paque Plus separation Ficoll-Paque PLUS gradient Ficoll-Paque separation Ficoll-Paque gradient Ficoll gradient separation Ficoll-Paque PLUS Ficoll-Plus gradient Ficoll-Paque Ficoll separation

2 protocols using ficoll paque plus gradient separation

1

Weekly Blood and BAL Sampling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Blood and BAL collections were performed weekly through the course of infection. An aliquot was taken for CFU measurement. Subsequently, PBMC were isolated using Ficoll-paque PLUS gradient separation (GE Healthcare Biosciences, Pittsburgh, PA). For BAL collections, tubing was inserted into the trachea with assistance using a laryngoscope, whereupon saline was instilled and immediately aspirated. An aliquot was taken for CFU and cells were isolated for flow cytometry analysis.
Kauffman K.D., Sallin M.A., Sakai S., Kamenyeva O., Kabat J., Weiner D., Sutphin M., Schimel D., Via L., Barry CE I.I.I., Wilder-Kofie T., Moore I., Moore R, & Barber D.L. (2017). Defective positioning in granulomas but not lung-homing limits CD4 T cell interactions with Mycobacterium tuberculosis infected macrophages in rhesus macaques. Mucosal immunology, 11(2), 462-473.
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2

Weekly Blood and BAL Sampling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Blood and BAL collections were performed weekly through the course of infection. An aliquot was taken for CFU measurement. Subsequently, PBMC were isolated using Ficoll-paque PLUS gradient separation (GE Healthcare Biosciences, Pittsburgh, PA). For BAL collections, tubing was inserted into the trachea with assistance using a laryngoscope, whereupon saline was instilled and immediately aspirated. An aliquot was taken for CFU and cells were isolated for flow cytometry analysis.
Kauffman K.D., Sallin M.A., Sakai S., Kamenyeva O., Kabat J., Weiner D., Sutphin M., Schimel D., Via L., Barry CE I.I.I., Wilder-Kofie T., Moore I., Moore R, & Barber D.L. (2017). Defective positioning in granulomas but not lung-homing limits CD4 T cell interactions with Mycobacterium tuberculosis infected macrophages in rhesus macaques. Mucosal immunology, 11(2), 462-473.
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