Hexadecyltrimethylammonium bromide (CTAB, >98.0%), L-ascorbic acid (BioUltra, ≥99.5%), silver nitrate (AgNO3, >99%), gold(III) chloride trihydrate (HAuCl4·3H2O, 99%), 3-mercaptopropionic acid (MPA, ≥99%), N-ethyl-N'-(3-dimethylaminopropyl)carbodiimide (EDC, 99%), and N-hydroxysuccinimide (NHs, 99%), cadmium chlorideanhydrous (CdCl2, 99.999%), selenium powder (Se, 99.8%), sodium borohydride (NaBH4, 99.99%), and zinc chloride anhydrous were purchased from Sigma-Aldrich. Sodium sulphide (Na2S, 60–63%) was purchased from Acros Organics, and anhydrous ethanol, sodium hydroxide (NaOH, AR) and hydrochloric acid (HCl, AR) were purchased from Sinopharm Chemical Reagent Co., Ltd. All chemicals were used as received without further purification. Deionized (DI) water used in all the studies was purified by a Milli-Q water purification system.
Anhydrous zinc chloride
Manufactured by Merck Group
Sourced in Germany
Anhydrous zinc chloride is a white, crystalline solid chemical compound with the formula ZnCl2. It is a water-soluble salt that is commonly used in various industrial and laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Ammonium molybdate tetrahydrate, by Merck Group (2 mentions)
Magnesium chloride anhydrous, by Merck Group (2 mentions)
Triton x 100, by Merck Group (2 mentions)
Phosphatase inhibitor cocktail 2, by Merck Group (2 mentions)
Resazurin sodium salt, by Merck Group (2 mentions)
Phosphate buffered saline (pbs), by Merck Group (2 mentions)
Sodium hydroxide (naoh), by Merck Group (2 mentions)
Selenium powder, by Merck Group (1 mentions)
Cadmium chloride anhydrous, by Merck Group (1 mentions)
3 mercaptopropionic acid, by Merck Group (1 mentions)
N ethyl n 3 dimethylaminopropyl carbodiimide, by Merck Group (1 mentions)
Sodium borohydride, by Merck Group (1 mentions)
L ascorbic acid, by Merck Group (1 mentions)
Silver nitrate, by Merck Group (1 mentions)
Gold 3 chloride trihydrate, by Merck Group (1 mentions)
N hydroxysuccinimide, by Merck Group (1 mentions)
Hexadecyltrimethylammonium bromide, by Merck Group (1 mentions)
Anhydrous ethanol, by Sinopharm (1 mentions)
Ammonium oxalate, by Merck Group (1 mentions)
Ammonium vanadate, by Merck Group (1 mentions)
9 protocols using anhydrous zinc chloride
1
Synthesis of Nanomaterials Using Wet Chemistry
2
Synthesis and Characterization of Inorganic Nanomaterials
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In this study, the following chemicals and materials were used. Ammonium hydroxide (NH4OH, 28%), ammonium vanadate (NH4VO3, 99%), ferric chloride anhydrous (FeCl3, 99.9%), zinc chloride anhydrous (ZnCl2, 99.9%), tert-butyl alcohol ((CH3)3COH, 98.5%), 1,4-benzoquinone (C6H4O2, 99%), and ammonium oxalate ((NH4)2C2O4, 99%) are provided from Sigma-Aldrich. The malachite green (MG) dye (C23H25N2Cl; MW = 364.92 g) is provided from Fluka. All chemicals used in this study were analytical grade and utilized without additional purification.
3
Stainless-Steel Sieve Particle Filtration
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A set of 5 stackable stainless-steel sieves of 8 cm high × 10 cm diameter and pore size of 3.5 mm, 1 mm, 300 μm, 100 μm and 20 μm (CISA, Lliçà de Vall, Spain) were used in the sampling procedure. Membrane filters of 1 μm pore 47 mm diameter polytetrafluoroethylene (PTFE) were from Savillex (Eden Prairie, MN, USA). A Vidrafoc glass filtration system (Barcelona, Spain) connected to a Vacuubrand vacuum pump (Essex, CT, USA) was used. In situ large volume water sampling in the DWTP was done using an immersible electro pump Hasa Inex-Palm (La Llagosta, Spain) adapted to eliminate plastic pieces and replace them with stainless-steel or PFTE.
Milli Q water was supplied by a MilliQ system (Millipore, Bedford, MA, USA). Ethanol for analysis grade was purchased from Merck (Darmstadt, Germany). Zinc chloride anhydrous was acquired from Sigma Aldrich (St. Louis, MO, USA).
Milli Q water was supplied by a MilliQ system (Millipore, Bedford, MA, USA). Ethanol for analysis grade was purchased from Merck (Darmstadt, Germany). Zinc chloride anhydrous was acquired from Sigma Aldrich (St. Louis, MO, USA).
4
Phosphatase Assay Protocol for Cell Cultures
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Sodium polyphosphate [PolyP, Graham's salt], PEI [molecular mass (mm) 25 kDa, branched], alkaline phosphatase from bovine intestinal mucosa (7165 units/mg protein), magnesium chloride anhydrous (MgCl2, 98%), zinc chloride anhydrous (ZnCl2, 97%), potassium phosphate monobasic (KH2PO4, 99.5%), phosphatase inhibitor cocktail 2, Triton X-100, ammonium molybdate tetrahydrate (81-83%), malachite green (MLG) oxalate salt (90%), fluorescein diacetate (FDA), minimum essential medium (MEM) eagle, glucose-d-(+) ≥99.5% (anhydrous), and resazurin sodium salt were purchased from Sigma-Aldrich (Vienna, Austria).
Fetal bovine serum (FBS), penicillin-streptomycin liquid and phosphate-buffered saline (PBS) were purchased from Biochrom, Merck (Tutzing, Germany). d-(+)-Trehalose dihydrate >98.0% (GC) was purchased from TCI Chemicals (Eschborn, Germany). 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) ≥99.5% was obtained from ROTH GmbH (Karlsruhe, Germany).
Fetal bovine serum (FBS), penicillin-streptomycin liquid and phosphate-buffered saline (PBS) were purchased from Biochrom, Merck (Tutzing, Germany). d-(+)-Trehalose dihydrate >98.0% (GC) was purchased from TCI Chemicals (Eschborn, Germany). 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) ≥99.5% was obtained from ROTH GmbH (Karlsruhe, Germany).
5
Chitosan-Polyphosphate Biomaterials Synthesis
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Chitosan (Cs) 25 kDa was purchased from Heppe Medical Chitosan GmbH (Halle, Germany) and sodium polyphosphate (PP) from Merck KGaA (Vienna, Austria). Alkaline phosphatase from bovine intestinal mucosa (7165 units/mg protein), ammonium molybdate tetrahydrate (8–83%), glucose-D-(+) ≥ 99.5% anhydrous, magnesium chloride anhydrous (MgCl2) ≥ 98%, minimum essential medium (MEM) eagle, malachite green oxalate salt 90%, potassium phosphate monobasic (KH2PO4) ≥ 99.5%, phosphatase inhibitor cocktail 2, resazurin sodium salt, rhodamine 123, Triton X-100, and anhydrous zinc chloride (ZnCl2) ≥ 97% were purchased from Sigma-Aldrich (Vienna, Austria). 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) ≥ 99.5% was purchased from ROTH GmbH (Karlsruhe, Germany). Fetal bovine serum (FBS) and phosphate-buffered saline (PBS) were purchased from Biochrom, Merck (Tutzing, Germany). D-(+)-Trehalose dehydrate ≥98.0% was purchased from TCI Chemicals (Eschborn, Germany).
6
Activated Carbon from Water Hyacinth
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The experimental research on plants, including the collection of plant material, complied with the relevant institutional, national, and international guidelines and legislation. The water hyacinth (Eichhornia Crassipes) was collected from Damanhour, Egypt as a raw source for activated carbon. Anhydrous zinc chloride (98%, ZnCl2), sodium hydroxide (NaOH), polyvinyl alcohol (PVA) 96.5% (M. Wt. 85,000–124,000 g/mol), and absolute ethanol (C2H5OH) were procured from Sigma-Aldrich. All chemicals were utilized without any further modifications. WI-38 normal lung fibroblast cells (ATCC® CCL-75™) were purchased from VACSERA CO., Cell Culture Research Unit, Giza, Egypt.
7
Chitosan-based Zinc Chloride Solution
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Chemical reagents were purchased through commercial vendors:
Chitosan, medium molecular weight (M, 210–300 kDa) and deacetylation degree 84% (Sigma-Aldrich, Darmstadt, Germany)
Anhydrous Potassium chloride (Sigma-Aldrich, Darmstadt, Germany)
Anhydrous Zinc chloride (Sigma-Aldrich, Darmstadt, Germany).
8
Synthesis and Characterization of Zinc Oxide Nanoparticles
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Cadmium nitrate tetrahydrate, sodium hydroxide,
methanol, sodium sulfide, acetone, 2-methylimidazole, anhydrous zinc
chloride, and methylene blue were purchased from Sigma-Aldrich and
used without further purification. Bacterial culture media including
Luria–Bertani (LB) and nutrient broth were procured from HiMedia
(India). Ampicillin was obtained from SRL (India). CellROX Deep Red
was obtained from Invitrogen. All other chemicals used were of analytical
grade and dissolved in Milli-Q water unless otherwise stated.
methanol, sodium sulfide, acetone, 2-methylimidazole, anhydrous zinc
chloride, and methylene blue were purchased from Sigma-Aldrich and
used without further purification. Bacterial culture media including
Luria–Bertani (LB) and nutrient broth were procured from HiMedia
(India). Ampicillin was obtained from SRL (India). CellROX Deep Red
was obtained from Invitrogen. All other chemicals used were of analytical
grade and dissolved in Milli-Q water unless otherwise stated.
9
Synthesis and Characterization of Metal-Organic Frameworks
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Europium chloride hexahydrate was purchased from Chemcraft (Russia). Benzene-1,4-dicarboxylic (terephthalic, H2bdc) acid (>98%) sodium hydroxide (>99%), polyethylene glycol 6000 (PEG-6000, for synthesis), iron(III) chloride hexahydrate (>99%), iron(II) sulphate heptahydrate (>99%), chromium(III) chloride hexahydrate (>99%), magnesium chloride hexahydrate (>99%), nickel(II) chloride hexahydrate (>99%), lead(II) nitrate (>99%), cobalt(II) chloride hexahydrate (>99%), anhydrous zinc chloride (>98%), cadmium chloride hydrate (>98%), barium chloride dihydrate (>99%), copper(II) chloride dihydrate (>99%), and EDTA disodium salt (0.1M aqueous solution) were purchased from Sigma-Aldrich Pty Ltd. (Germany) and used without additional purification. The 0.2 M solutions of the above-mentioned salts were prepared and standardized by complexometric titration with EDTA. An amount of 0.3 moles of the terephthalic acid and 0.6 moles of the sodium hydroxide were dissolved in the distilled water to obtain 1 L of 0.3 M solution of the disodium terephthalate (Na2bdc).
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