Sc 32245

Manufactured by Novus Biologicals

Sc-32245 is a lab equipment product offered by Novus Biologicals. It is used for research purposes, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

Automatically generated - may contain errors

Lab products found in correlation

Bca reagent, by Bio-Rad (2 mentions) A2856, by Santa Cruz Biotechnology (2 mentions) β actin, by Merck Group (2 mentions) Nb600 1384, by Abcam (1 mentions) 03 gp62 c, by Abcam (1 mentions) Tom20, by Abcam (1 mentions) Ab 108327, by Cell Signaling Technology (1 mentions) Paccs79, by Cell Signaling Technology (1 mentions) Ps6 s235 236, by Cell Signaling Technology (1 mentions) 03 gp62 c, by Cell Signaling Technology (1 mentions)

2 protocols using sc 32245

Protein Extraction and Western Blotting

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Tumor samples were snap-frozen, ground using Cryomill in liquid nitrogen at 25 Hz for 2 min, and lysed in Tris lysis buffer. Protein concentration was assessed using the Bio-Rad BCA reagent. The following antibodies were used for Western blots: Atg7 (Sigma Aldrich, A2856), Lkb1 (Santa Cruz Biotechnology, sc-32245), LC3 (Novus Biologicals, NB600-1384), Atg5 (Abcam, ab108327), p62 (American Research Products, 03-GP62-C), TOM20 (Abcam, ab186735), and β-actin (Sigma, A1978).

Bhatt V., Khayati K., Hu Z.S., Lee A., Kamran W., Su X, & Guo J.Y. (2019). Autophagy modulates lipid metabolism to maintain metabolic flexibility for Lkb1-deficient Kras-driven lung tumorigenesis. Genes & Development, 33(3-4), 150-165.

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Western Blot Analysis of Autophagy Proteins

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Tissues were snap-frozen in liquid nitrogen, ground using Cryomill in liquid nitrogen at 25 Hz for 2 min, and then lysed in Tris lysis buffer (1M Tris-HCl, 1M NaCl, 0.1M EDTA, 10% NP40). Protein concentrations were measured using the Bio-Rad BCA reagent. Samples were probed with antibodies against Atg7 (Sigma Aldrich, A2856, RRID:AB_1078239), Lkb1 (Santa Cruz Biotechnology, sc-32245, RRID:AB_627890), LC3 (Novus Biologicals, NB600-1384, RRID:AB_669581), p62 (American Research Products, 03-GP62-C, RRID:AB_1542690), p-ACC^S79 (Cell Signaling, 3661, RRID:AB_330337), ACC (Cell Signaling, 3676, RRID:AB_2219397), Atg5 (Abcam, ab108327, RRID:AB_2650499), p-S6^S235/236 (Cell Signaling, 4858, RRID:AB_916156), S6 (Cell Signaling, 2217, RRID:AB_331355), and β-actin (Sigma Aldrich, A1978, RRID:AB_476692). Western blots were quantified with Image J (National Institutes of Health). The intensities of bands were used to calculate relative ratios of the indicated protein over loading control (β-actin), which were then normalized based on the corresponding ratio in the wild type control sample.

Khayati K., Bhatt V., Hu Z.S., Fahumy S., Luo X, & Guo J.Y. (2020). Autophagy compensates for Lkb1 loss to maintain adult mice homeostasis and survival. eLife, 9, e62377.

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