Log In Sign up
The largest database of trusted experimental protocols

Ceramic 2.8mm

Manufactured by Qiagen
Visit Supplier

The Ceramic 2.8mm is a laboratory equipment product designed for various applications. It is a ceramic bead with a diameter of 2.8 millimeters. The core function of this product is to provide a controlled and consistent surface for use in various laboratory processes, such as sample preparation and homogenization.

Automatically generated - may contain errors

Lab products found in correlation

Powerbead tube, by Qiagen (2 mentions) Complete mini protease inhibitor cocktail, by Merck Group (2 mentions) Powerlyzer 24 homogenizer, by Qiagen (2 mentions)

2 protocols using ceramic 2.8mm

1

Protein Extraction and Multiplexed ELISA

Check if the same lab product or an alternative is used in the 5 most similar protocols
0.1% SDS; 1mM EDTA; 1% NP-40, supplemented with fresh protease inhibitors, Complete™ Mini Protease Inhibitor Cocktail, Sigma) using PowerBead Tubes, Ceramic 2.8mm and PowerLyzer 24 Homogenizer (110/220 V, 2 × 30-sec cycles; S 3500) (Qiagen). After homogenization, RIPA lysates were incubated for 30 mins at 4°C and with continued vortexing, and clarified by centrifugation. Same amounts of total tissue protein (25 µg) diluted in RIPA buffer were subjected to quantitative multiplexed ELISA (Discovery Assays) performed by Eve Technologies (Canada).
Alonso-Curbelo D., Ho Y.J., Burdziak C., Maag J.L., Morris JP I.V., Chandwani R., Chen H.A., Tsanov K.M., Barriga F.M., Luan W., Tasdemir N., Livshits G., Azizi E., Chun J., Wilkinson J.E., Mazutis L., Leach S.D., Koche R., Pe’er D, & Lowe S.W. (2021). A gene-environment induced epigenetic program initiates tumorigenesis. Nature, 590(7847), 642-648.
+ Open protocol
+ Expand
2

Protein Extraction and Multiplexed ELISA

Check if the same lab product or an alternative is used in the 5 most similar protocols
0.1% SDS; 1mM EDTA; 1% NP-40, supplemented with fresh protease inhibitors, Complete™ Mini Protease Inhibitor Cocktail, Sigma) using PowerBead Tubes, Ceramic 2.8mm and PowerLyzer 24 Homogenizer (110/220 V, 2 × 30-sec cycles; S 3500) (Qiagen). After homogenization, RIPA lysates were incubated for 30 mins at 4°C and with continued vortexing, and clarified by centrifugation. Same amounts of total tissue protein (25 µg) diluted in RIPA buffer were subjected to quantitative multiplexed ELISA (Discovery Assays) performed by Eve Technologies (Canada).
Alonso-Curbelo D., Ho Y.J., Burdziak C., Maag J.L., Morris JP I.V., Chandwani R., Chen H.A., Tsanov K.M., Barriga F.M., Luan W., Tasdemir N., Livshits G., Azizi E., Chun J., Wilkinson J.E., Mazutis L., Leach S.D., Koche R., Pe’er D, & Lowe S.W. (2021). A gene-environment induced epigenetic program initiates tumorigenesis. Nature, 590(7847), 642-648.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!