Human galectin 3bp elisa kit

Beta-Lapachone (ARQ-501, LPC) was purchased from Selleck Chemicals Inc. RIPA buffer solution, TRIzol reagent, RIPA buffer solution and endotoxin-free plasmid small extraction medium volume extraction kit were obtained from Sparkjade Biotech (Shandong, China) AceQ qPCR SYBR Green Master Mix was purchased from Vazyme (Nanjing, China), and First-strand cDNA Synthesis super Mix kit was from TransGen Biotech (Beijing, China). HE staining kit was obtained from Beyotime Biotechnology (Hangzhou, China). EdU incorporation assay kit was obtained from Keygen Biotech (shanghai, China). Fetal Bovine Serum was purchased from Sigma-Aldrich (St. Louis, MO, United States). VECTASTAIN ABC Kit was purchased from Vector Laboratories. Human Galectin-3BP ELISA Kit was purchased from Abcam (Cambridge, MA, United States). Human IL6, IL6R ELISA kit were purchased from Boster Biological Technology (Nanjing, China). Primary antibodies we used were shown as following: ERK1/2 and β-Actin were purchased from abway. Gal-3, pSTAT3 and pERK1/2 were purchased from Cell Signaling Technology. Gal-3BP (LGALS3BP) was purchased from R&D Systems (Minneapolis, MN). STAT3 was purchased from Proteintech Group.

Levels of complement C3 and galectin‐3 binding protein in plasma were measured using the Human Complement C3 ELISA kit (ab108823; Abcam, Cambridge, UK) and the Human Galectin‐3BP ELISA kit (ab213784; Abcam), respectively. The level of apolipoprotein C‐III in plasma was measured by a direct ELISA method as previously described 21.