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Primers for rt pcr

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Primers for RT-PCR are short, single-stranded DNA molecules designed to serve as starting points for the reverse transcription and subsequent amplification of specific RNA sequences using the reverse transcription-polymerase chain reaction (RT-PCR) technique. These primers are essential components of the RT-PCR process, allowing for the detection and quantification of target RNA molecules in a sample.

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Lab products found in correlation

Human insulin, by Merck Group (2 mentions) Sodium oleate, by Merck Group (2 mentions) Ntr1 antibody sc 374492, by Santa Cruz Biotechnology (2 mentions) F4 80 ab100790, by Abcam (2 mentions) Nt1 13, by Merck Group (2 mentions) Deuterated oleic acid clm 460 pk, by Cambridge Isotopes (2 mentions) Lipofectamine rnaimax and ltx reagent with plus transfection reagents, by Thermo Fisher Scientific (2 mentions) Pan akt 4691, by Cell Signaling Technology (2 mentions) Flag f1804, by Merck Group (2 mentions) β actin a5316, by Merck Group (2 mentions) Aicar, by Cayman Chemical (2 mentions) Sr 48692, by Bio-Techne (2 mentions)

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PCR primers (44 citations) Primers for PCR (2 citations)

2 protocols using primers for rt pcr

1

Optimizing Metabolic Signaling in Cell Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols
Phospho-AMPKα (Thr172) (2535), AMPKα (2532), LKB1 (3050), phospho-Akt (Ser-473) (4058) and pan-Akt (4691) antibodies were from Cell Signaling Technology (Danvers, MA). NTR1 antibody (sc-374492) was from Santa Cruz Biotechnology (Dallas, TX). CaMKK2 (ab168818), NTR3 (ab16640), and F4/80 (ab100790) antibodies were from Abcam (Cambridge, MA). FLAG (F1804) and β-actin (A5316) antibodies were from Sigma-Aldrich (St. Louis, MO). AICAR was from Cayman (Ann Arbor, MI). Oleate sodium, NT1–13, glucose and human insulin were from Sigma. Deuterated oleic acid (CLM-460-PK) was obtained from Cambridge Isotope Laboratories (Tewksbury, MA). SR 48692 was from Tocris (Minneapolis, MN). Lipofectamine® RNAiMAX and LTX Reagent with PLUS™ transfection reagents and Trizol were from Life Technologies (Grand Island, NY). pSG5-FLAG-CaMKK2 rat FL was a gift from Anthony Means30 (link) (Addgene plasmid #32449, Cambridge, MA). Primers for RT-PCR were from Integrated DNA Technologies (Coralville, IA).
Li J., Song J., Zaytseva Y.Y., Liu Y., Rychahou P., Jiang K., Starr M.E., Kim J.T., Harris J.W., Yiannikouris F.B., Katz W.S., Nilsson P.M., Orho-Melander M., Chen J., Zhu H., Fahrenholz T., Higashi R.M., Gao T., Morris A.J., Cassis L.A., Fan T.W., Weiss H.L., Dobner P.R., Melander O., Jia J, & Evers B.M. (2016). An obligatory role for neurotensin in high fat diet-induced obesity. Nature, 533(7603), 411-415.
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2

Optimizing Metabolic Signaling in Cell Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols
Phospho-AMPKα (Thr172) (2535), AMPKα (2532), LKB1 (3050), phospho-Akt (Ser-473) (4058) and pan-Akt (4691) antibodies were from Cell Signaling Technology (Danvers, MA). NTR1 antibody (sc-374492) was from Santa Cruz Biotechnology (Dallas, TX). CaMKK2 (ab168818), NTR3 (ab16640), and F4/80 (ab100790) antibodies were from Abcam (Cambridge, MA). FLAG (F1804) and β-actin (A5316) antibodies were from Sigma-Aldrich (St. Louis, MO). AICAR was from Cayman (Ann Arbor, MI). Oleate sodium, NT1–13, glucose and human insulin were from Sigma. Deuterated oleic acid (CLM-460-PK) was obtained from Cambridge Isotope Laboratories (Tewksbury, MA). SR 48692 was from Tocris (Minneapolis, MN). Lipofectamine® RNAiMAX and LTX Reagent with PLUS™ transfection reagents and Trizol were from Life Technologies (Grand Island, NY). pSG5-FLAG-CaMKK2 rat FL was a gift from Anthony Means30 (link) (Addgene plasmid #32449, Cambridge, MA). Primers for RT-PCR were from Integrated DNA Technologies (Coralville, IA).
Li J., Song J., Zaytseva Y.Y., Liu Y., Rychahou P., Jiang K., Starr M.E., Kim J.T., Harris J.W., Yiannikouris F.B., Katz W.S., Nilsson P.M., Orho-Melander M., Chen J., Zhu H., Fahrenholz T., Higashi R.M., Gao T., Morris A.J., Cassis L.A., Fan T.W., Weiss H.L., Dobner P.R., Melander O., Jia J, & Evers B.M. (2016). An obligatory role for neurotensin in high fat diet-induced obesity. Nature, 533(7603), 411-415.
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