Rm il 3

Manufactured by ProSpec

Sourced in United States

The Rm-IL-3 is a laboratory instrument designed for the measurement and analysis of interleukin-3 (IL-3) levels in biological samples. It utilizes a specific detection method to quantify the concentration of IL-3 present in the sample.

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Lab products found in correlation

Rm flt3 ligand, by ProSpec (3 mentions) Actrapid, by Novo Nordisk (3 mentions) Rh il 6, by ProSpec (3 mentions) Methocult base medium, by STEMCELL (2 mentions) Human holo transferrin, by ProSpec (2 mentions) Ls column, by Miltenyi Biotec (1 mentions) Penicillin, by ProSpec (1 mentions) L glutamine, by ProSpec (1 mentions) Rm scf, by ProSpec (1 mentions) M3134, by STEMCELL (1 mentions)

3 protocols using rm il 3

Lineage Depletion and Colony Assays

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BM lineage depletion was performed using biotinylated antibodies against
red cell precursors (αTer119), B cells (αCD19), T cells
(αCD3ε) and myeloid cells (αGr1), MACS αbiotin beads
and LS columns (Miltenyi Biotec). Colony formation assays were performed as
previously described^{40 (link)}.
Briefly, 3.3 × 10³ Lin⁻ cells were cultured
in MethoCult base medium (STEMCELL Technologies) supplemented with 15% FCS, 20%
BIT (50 mg/ml BSA in Iscove’s modified Dulbecco’s medium, 1.44
U/ml rh-insulin [Actrapid, Novo Nordisk], and 250 ng/ml human Holo Transferrin
[ProSpec]), 100 μM 2-β-mercaptoethanol, 100 U/ml penicillin, 100
μg/ml streptomycin, 2 mM L-glutamine, and a cytokine mix of 50 ng/ml
rm-SCF, 10 ng/ml rm-IL-3, 10 ng/ml rh-IL-6, and 50 ng/ml rm-Flt3-ligand (all
from ProSpec). Total colonies and colony subsets were enumerated after 7
days.
Mesenchymal stem cells (MSCs) were analysed by flow cytometry as
previously described^{40 (link)}.
Briefly, bones were crushed and digested in PBS with collagenase and DNase for 1
h at 37°C. Stromal cells were separated from the hematopoietic fraction
by removing CD45⁺Ter-119⁺ cells by MACS. Cells were
stained for CD51, Sca-1, and CD31. Hematopoietic cells were excluded with
lineage markers, CD45, and Ter-119.

Brigger D., Riether C., van Brummelen R., Mosher K.I., Shiu A., Ding Z., Zbären N., Gasser P., Guntern P., Yousef H., Castellano J.M., Storni F., Graff-Radford N., Britschgi M., Grandgirard D., Hinterbrandner M., Siegrist M., Moullan N., Hofstetter W., Leib S.L., Villiger P.M., Auwerx J., Villeda S.A., Wyss-Coray T., Noti M, & Eggel A. (2020). Eosinophils regulate adipose tissue inflammation and sustain physical and immunological fitness in old age. Nature metabolism, 2(8), 688-702.

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Murine Hematopoietic Progenitor Assay

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A total of 5 × 10³ lin⁻ BM cells were cultured in MethoCult base medium (STEMCELL Technologies, Grenoble, France) supplemented with 15% FCS, 20% BIT (50 mg ml⁻¹ BSA in Iscove's modified Dulbecco's medium, 1.44 U ml⁻¹ rh-insulin (Actrapid, Novo Nordisk, Denmark), and 250 ng ml⁻¹ human Holo Transferrin [ProSpec, USA]), 100 μM 2-β-mercaptoethanol, 100 U ml⁻¹ penicillin, 100 μg ml⁻¹ streptomycin, 2 mM L-glutamine, and a cytokine mix of 50 ng ml⁻¹ rm-SCF, 10 ng ml⁻¹ rm-IL-3, 10 ng ml⁻¹ rh-IL-6, and 50 ng ml⁻¹ rm-Flt3-ligand (all from ProSpec, USA). Colonies were enumerated after 7 days of culture. Colony subtypes were determined by inverted light microscopy and by Pappenheim staining of individually plucked colonies.

Zysset D., Weber B., Rihs S., Brasseit J., Freigang S., Riether C., Banz Y., Cerwenka A., Simillion C., Marques-Vidal P., Ochsenbein A.F., Saurer L, & Mueller C. (2016). TREM-1 links dyslipidemia to inflammation and lipid deposition in atherosclerosis. Nature Communications, 7, 13151.

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Hematopoietic Progenitor Cell Assay

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3.33×10³ lin⁻ cells were transferred into methocult base medium (M3134; Stemcell Technologies) supplemented with 15% FCS, 20% BIT (50 mg/ml BSA in IMDM, 1.44 U/ml recombinant-human (rh) insulin (Actrapid, Novo Nordisk) and 250 ng/ml human holo transferrin [Prospec]), 100 µM 2-β-mercaptoethanol, 2 mM l-glutamine, penicillin/streptomycin, and 50 ng/ml recombinant-mouse SCF (rmSCF), 10 ng/ml rm–IL-3, 10 ng/ml rh-IL-6 and 50 ng/ml rm-Flt3-ligand (all from Prospec). Colonies and cells were enumerated after 7 days (≥30 cells/colony).

Weber B., Schuster S., Zysset D., Rihs S., Dickgreber N., Schürch C., Riether C., Siegrist M., Schneider C., Pawelski H., Gurzeler U., Ziltener P., Genitsch V., Tacchini-Cottier F., Ochsenbein A., Hofstetter W., Kopf M., Kaufmann T., Oxenius A., Reith W., Saurer L, & Mueller C. (2014). TREM-1 Deficiency Can Attenuate Disease Severity without Affecting Pathogen Clearance. PLoS Pathogens, 10(1), e1003900.

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