Wst 1

Manufactured by Agilent Technologies

Sourced in United States

The WST-1 is a water sample tester that measures the absorbance of a water sample at 420-480 nm wavelength. It provides a quantitative assessment of the water sample's turbidity or cloudiness. The device is designed for laboratory use and can be utilized for various water quality testing applications.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

WST-1 reagent (8 citations) Cell Proliferation Reagent WST-1 (2 citations)

25 protocols using wst 1

Assessing MCF-7 Cell Viability

Check if the same lab product or an alternative is used in the 5 most similar protocols

The viability of MCF-7 cells was assessed using the water-soluble tetrazolium (WST)-1 (Roche Molecular Biochemicals, Mannheim, Germany). The cells (10,000 cells/well) were seeded in 96-well plates for 24 hr, followed by treatment with CPAE, E2, ICI, or a combination of these compounds and then they were incubated for 3 days. Based on the manufacturer’s instructions, we added 10 μL of the WST-1 cell proliferation reagent to each well, incubated the plates for 2 hr at 37°C, and then the cell viability was quantified by measuring the absorbance at 440 nm using a BioTek Synergy HT microplate reader (BioTek Instruments, Winooski, USA).

Kim S.J., Jin S.W., Lee G.H., Kim Y.A, & Jeong H.G. (2017). Evaluation of Estrogenic Activity of Extract from the Herbal Mixture Cynanchum wilfordii Hemsley, Phlomis umbrosa Turczaninow, and Angelica gigas Nakai. Toxicological Research, 33(1), 71-77.

+ Open protocol

+ Expand

WST-1 Assay for Cell Viability

Check if the same lab product or an alternative is used in the 5 most similar protocols

For WST-1 analysis, 6,000 MCF-7 cells were seeded in 100 μL medium per well in 96-well culture plates. The following day, the cells were treated for 48 or 72 h by adding 100 μL medium containing indicated compounds. WST-1 (20 μL, Roche) was added when 4 h of the treatment remained. Cell viability was analyzed by a WST-1 assay as described by the manufacturer’s protocol using a Synergy 2 Microplate Reader and Gen5 Reader Control and Data Analysis software (BioTek).

Granqvist V., Holmgren C, & Larsson C. (2021). Induction of interferon-β and interferon signaling by TRAIL and Smac mimetics via caspase-8 in breast cancer cells. PLoS ONE, 16(3), e0248175.

+ Open protocol

+ Expand

Cellular Metabolic Activity Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell-permeable redox indicators Resazurin (Biotium, Cat# 30025-1) and WST-1 (Roche, Cat# 5015944001 were used according to manufacturer protocols to monitor cellular metabolic activity, which is indicative of cell viability. NT-Control and DRP1 KD macrophages were seeded onto 96-well plates for the Resazurin assay or 24-well plates for the WST-1 assay at a density of 5×10⁴ or 2.5×10⁵/well, respectively. On the next day, cells were left untreated (Mock) or stimulated with LPS (200 ng/ml) and cellular metabolic redox was monitored. Absorbance was measured at 570 nm and 600 nm for the reduced Resazurin (Resorufin) and at 440 and 600 nm for the reduced WST-1 (Formazan) using a BioTek microplate reader.

Gao F., Reynolds M.B., Passalacqua K.D., Sexton J.Z., Abuaita B.H, & O’Riordan M.X. (2021). The Mitochondrial Fission Regulator DRP1 Controls Post-Transcriptional Regulation of TNF-α. Frontiers in Cellular and Infection Microbiology, 10, 593805.

+ Open protocol

+ Expand

Cell Viability Assay with WST-1

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells (6000 per well) were seeded in 100 µL complete medium in 96-well culture plates. After 24 h, 100 µL complete medium containing indicated compounds were added to the wells. During the final 4 h, 20 µL WST-1 (Roche, Basel, Switzerland) was added to each well. The amount of viable cells was analyzed with a WST-1 assay using a Synergy 2 Microplate Reader and Gen5 Reader Control and Data Analysis software v.1.02.8 (BioTek, Winooski, VT, USA).

Holmgren C., Sunström Thörnberg E., Granqvist V, & Larsson C. (2022). Induction of Breast Cancer Cell Apoptosis by TRAIL and Smac Mimetics: Involvement of RIP1 and cFLIP. Current Issues in Molecular Biology, 44(10), 4803-4821.

+ Open protocol

+ Expand

Cell Viability Assay for Glioblastoma

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell viability for GSCs (83Mes and AC17PN) and the established GBM cell lines (U87, LN229, and LN18) was assessed using the Cell Proliferation Reagent WST-1 (Roche) according to the manufacturer’s instructions. Briefly, cells were seeded into 96-well plates at a density of 3,000 cells per well in the presence of the indicated drugs. Cells were incubated at 37 C in 5% CO₂. After 5 days, the WST-1 reagent was added (10%, v/v) and cell viability was assessed using the Synergy HT plate reader and the Gen5 software (BioTek).
For ex vivo PDX GBM cells, 2,500 cells were plated per well in a 384-well tissue culture treated plate (Greiner) in 25 μL of supplemented Neurobasal medium. Twenty-five nanoliters of 1,000 drugs were added using a Labcyte Echo acoustic dispensing device and the cells were incubated for 96 hours. Cell viability was assessed using CellTiterGlo (Promega) on a Synergy 2 plate reader with Gen5 software (BioTek).

Bell J.B., Eckerdt F., Dhruv H.D., Finlay D., Peng S., Kim S., Kroczynska B., Beauchamp E.M., Alley K., Clymer J., Goldman S., Cheng S.Y., James C.D., Nakano I., Horbinski C., Mazar A.P., Vuori K., Kumthekar P., Raizer J., Berens M.E, & Platanias L.C. (2017). Differential Response of Glioma Stem Cells to Arsenic Trioxide Therapy Is Regulated by MNK1 and mRNA Translation. Molecular cancer research : MCR, 16(1), 32-46.

+ Open protocol

+ Expand

Biocompatibility Evaluation of Electrosprayed hHL Particles

Check if the same lab product or an alternative is used in the 5 most similar protocols

hHL and RS-loaded hHL electrosprayed particles (hHL/0.05RS and hHL/0.1RS) sprayed onto round glass slides, were tested to assess biocompatibility with human dermal fibroblast cell lines (PCS-201-012) via WST-1 (4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzenedisulfonate) (Roche Applied Science, Mannheim, Germany) cell proliferation and viability assay. For this, surfaces were presterilized by 2% penicillin-streptomycin in PBS for 30 min and were suspended in DMEM. After the sterilization process, sprayed surfaces were placed in 48 well plates, and dermal fibroblast cells at the 70% confluency were seeded onto samples at the density of 5 × 10⁴ cell/well and incubated for 24 h at 37 °C in humidified air containing 5% CO₂. At the end of the incubation period, WST-1 reagent was added onto wells and incubated for 2 h in the dark at 37 °C in 5% CO_2, and absorbance was measured at 405 nm by Biotek Cytation 3 (Winooski, VT, USA). Untreated cells (cells on wells without any sample) were used as control and considered 100% viable.

Cinan E., Cesur S., Erginer Haskoylu M., Gunduz O, & Toksoy Oner E. (2021). Resveratrol-Loaded Levan Nanoparticles Produced by Electrohydrodynamic Atomization Technique. Nanomaterials, 11(10), 2582.

+ Open protocol

+ Expand

Quantifying Cell Proliferation and Colony Formation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell proliferation assay was measured with WST-1 (promega) reagent
according to the manufacturer’s instruction (Clontech). Briefly,
cells were treated with WST-1 for 2hours at 37oC incubator prior to
absorbance reading at 440nm using the KC4 microplate reader (BioTek). Each
absorbance was normalized to the media control without any cells. For the
Incucyte cell confluence assay, C4–2B cells were infected with
pLKO.1V, shEZH2, shSUZ12 or shAR for 24 hours and harvested by
trypsinization. 5,000 cells were counted on a Countess automated cell
counter (Life Technologies, Carlsbad, CA) and plated on 24 tissue culture
plates in 3 replicates. Photomicrographs were taken every two hours using an
Incucyte live cell imager (Essen Biosciences, Ann Arbor, MI). Cell
confluence were measured using Incucyte software (Essen Biosciences, Ann
Arbor, MI) over 5 days in culture. Data were normalized to the pLKO.1
control cells and analyzed using Incucyte software (Essen Biosciences, Ann
Arbor, MI).
For colony formation assay, 1,000–2,000 cells were plated in
each well of a 6-well plate and treated with indicated concentration of
DMSO, Enz, EPZ or both for 10–14 days, cells were fixed by 4%
paraformaldehyde and stained by 0.05% crystal violet.

Kim J., Lee Y., Lu X., Song B., Fong K.W., Cao Q., Licht J.D., Zhao J.C, & Yu J. (2018). Polycomb- and Methylation-Independent Roles of EZH2 as a Transcription Activator. Cell reports, 25(10), 2808-2820.e4.

+ Open protocol

+ Expand

Evaluating HUVEC Proliferation with Iopromide-Coated Balloons

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human umbilical vein endothelial cells (HUVECs) were seeded at low density (30,000 cells per 12-well culture plates) and cultured in M200 supplemented with 10% fetal bovine serum (FBS) and growth factors for 24 h. Cells were then co-incubated with an iopromide-coated balloon without paclitaxel (

n = 6

) for 2 days. HUVEC cultures were incubated with a bare balloon as controls (

n = 6

). After the incubation period, 50 µl of cell proliferation reagent, WST-1 (Roche Applied Science, Mannheim, Germany) was added to each well. After 4 h of WST-1 addition, absorbance at 450 nm was measured via an enzyme-linked immunosorbent assay (ELISA) plate reader (BioTek, Winooski, VT, USA).

Zhu Z., Han H., Zhu J., Zhang J., Du R., Ni J., Ying C., An X, & Zhang R. (2015). Safety and efficacy of a novel iopromide-based paclitaxel-eluting balloon following bare metal stent implantation in rabbit aorta abdominalis. Bio-Medical Materials and Engineering, 26(1-2), 79-88.

+ Open protocol

+ Expand

Cell Proliferation Assay with WST-1 and 3-MA

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell proliferation assays were performed using WST-1 (Takara, Otsu, Japan) according to the manufacturer's instruction. Briefly, B16F1 or B16F10 cells (1 × 10⁴ per well) were plated in 96-well microplates for one day and then treated with IMQ after pretreatment with 3-MA (10 mM). At each time point, the WST-1 reagent was added to the 96-well plates, and the plates were then incubated for 4 h. The absorbances at 450 nm and/or 690 nm were measured using a plate reader (Biotek Instruments Inc., Winoski, VT, USA).

Cho J.H., Lee H.J., Ko H.J., Yoon B.I., Choe J., Kim K.C., Hahn T.W., Han J.A., Choi S.S., Jung Y.M., Lee K.H., Lee Y.S, & Jung Y.J. (2017). The TLR7 agonist imiquimod induces anti-cancer effects via autophagic cell death and enhances anti-tumoral and systemic immunity during radiotherapy for melanoma. Oncotarget, 8(15), 24932-24948.

+ Open protocol

+ Expand

WST-1 Cell Proliferation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

WST-1 (5015944001, Sigma) was used for the cell proliferation assays according to the manufacturer’s instructions. Briefly, the cells were seeded in 96-well plates and maintained at 37 °C and 5% CO₂ for 24 h before further processing. Ten microliters per well of cell proliferation reagent WST-1 was added to the cells cultured in 100 μl/well, and the cells were incubated between 0.5~4 h. The amount of formazan dye produced was determined by measuring the absorbance at 450 nm using an imaging reader (Cytation|5, BioTek).

Zhang Z., Jing J., Ye Y., Chen Z., Jing Y., Li S., Hong W., Ruan H., Liu Y., Hu Q., Wang J., Li W., Lin C., Diao L., Zhou Y, & Han L. (2020). Characterization of the dual functional effects of heat shock proteins (HSPs) in cancer hallmarks to aid development of HSP inhibitors. Genome Medicine, 12, 101.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!