Cobas 8000 c701

On Sysmex Sysmex CS-5100 (Sysmex Corporation, Kobe, Japan): prothrombin time-international normalized ratio (Medirox Owren´s PT reagent), activated partial thromboplastin time (aPTT) (Dade Actin FS activated PTT reagent, Siemens), fibrinogen (Clauss method, Dade Thrombin Reagent, Siemens), D-dimer (Innovance D-Dimer, Siemens), soluble fibrin monomers (Sta-Liatest FM, Stago). Platelet count was performed on Sysmex XN (Sysmex Corporation, Kobe, Japan). Plasma albumin was performed on Cobas 8000 c701 (Roche Diagnostics) with BCP (Bromocresol Purple) reagent (Roche Diagnostics).
On a SpectraMax® i3x Multi-Mode microplate reader: thrombin-antithrombin complexes (Enzygnost TAT micro Kit, Siemens) and fibrinopeptide B (Human Fibrinopeptide B Elisa Kit, Novus Biologicals).
Thromboelastometry was performed using a ROTEM® delta device (Pentapharm GmbH, Munich, Germany).

Fasting blood concentrations of vitamin D (25(OH) D), triglycerides (TG), total cholesterol (TC), HDL cholesterol (HDL-c), LDL cholesterol (LDL-c), fasting blood glucose (FBG), HbA1c, HOMA-IR, and PTH were the secondary outcomes. Serum concentrations of (25(OH) D) were completed using radioimmunoassay (DiaSorin, Stillwater, MN, USA); inter-assay CV <8% and intra-essay CV ∼4.9%. Our laboratory normal values for (25(OH) D) are 33–90 ng/mL. Hypovitaminosis is identified with a result <20 ng/mL (taking IOM references). TG, TC, HDL-c and LDL-c were measured enzymatically using a RA1000 autoanalyzer (Bayer Diagnostics, Suffolk, UK). FBG was accomplished through a hexokinase assay on blood transferred into specific tubes that are fluoridated; inter-assay CV 1.8% at 6.6 mmol/L. The hexokinase method, established by the American Association for Clinical Chemistry, is used most extensively in clinical research, and has been recognized as the reference method to assess blood glucose [32 (link),33 (link)]. The values of HbA1c were evaluated using a Roche, Cobas 8000 (C701 and C702) analyzer. Insulin resistance (IR) was calculated by the homeostasis model assessment of insulin resistance using the following formula: HOMA-IR = [insulin (mU/L) × glucose (mg/dL)]/22.5. PTH was assessed by immunoradiometric assay (a two side one) including a NH₂-terminal antibody meant for conservation.

AST and urea levels were measured using Cobas-8000-C701 (Roche) by LABOKLIN (Bad Kissingen, Germany).

Venous blood samples were drawn after an overnight fast. Separated plasma or serum was frozen in aliquots and stored at −80°C until thawed for the first time for the analyses. The SUA level was measured by oxidization with the specific enzyme uricase on a Chemistry Analyzer (ROCHE Cobas8000C701, USA). HUA was defined as SUA > 420 μmol/L (7 mg/dl) based on the Guideline for primary care of gout and HUA in China (version 2019) (19 (link)).

Sodium, potassium, and calcium were measured in plasma and saliva and phosphate in saliva. Total plasma cholesterol, high- and low-density lipoprotein (HDL and LDL, respectively), cholesterol, and triglycerides were also measured. The salivary electrolytes were measured in centrifuged saliva samples on a Cobas 8000 c701 chemistry analyzer (Roche Diagnostics) at the Karolinska University Laboratory, Karolinska University Hospital, Solna, Sweden.

All laboratory markers were measured in the local laboratory (Labordiagnostisches Zentrum, LDZ) at University Hospital RWTH Aachen. Circulating levels of serum tumor markers were analyzed with an electrochemiluminescence immunoassay (ECLIA) using the Cobas 8000 e602 modular analyzer series (Hoffmann-La Roche AG, Basel, Switzerland). Standard hematological and clinical chemistry parameters were measured using the Sysmex XN9000 (Sysmex GmbH, Norderstedt, Germany) and the Cobas 8000 c701 (Hoffmann-La Roche AG, Basel, Switzerland).

The CRP concentrations were measured in samples of the patient ‘s blood serum by an immunologic particle-enhanced turbidimetric test on Cobas 6000^® c501 or Cobas 8000^® c701 machines (Roche Diagnostics, Mannheim, Germany). In this test, human CRP forms aggregates with latex particles covered with monoclonal anti-CRP antibodies (Cobas, V 7.0), thereby producing a measurable turbidity. IL-6 concentrations were measured by an electric chemiluminescence immunoassay (ECLIA) on Cobas 8000^® machines.