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Quantichrom urea assay kit diur 500

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The QuantiChrom™ Urea Assay Kit (DIUR-500) is a colorimetric assay designed to quantify urea levels in biological samples. The kit utilizes a chromogenic reagent that produces a colored complex when reacted with urea, allowing for spectrophotometric measurement and determination of urea concentrations.

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Lab products found in correlation

Enzymatic reagent set, by Horiba (3 mentions) Albuwell m, by Exocell (3 mentions) Coomassie stain, by Bio-Rad (2 mentions) Creatinine companion, by Exocell (2 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Quantichrom creatinine assay kit dict 500, by BioAssay Systems (1 mentions) Creatinine assay kit, by Abcam (1 mentions) Albuwell m kit, by Exocell (1 mentions)

9 protocols using quantichrom urea assay kit diur 500

1

Urinary Biomarkers for Kidney Function Assessment

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Urinary albumin levels were determined by ELISA using a murine microalbuminuria ELISA kit, AlbuwellM™ (Exocell Inc., Philadelphia, PA, USA). The urine creatinine concentration was measured with a microplate assay kit, Creatinine Companion (Exocell Inc., Philadelphia, PA, USA). All measurements were performed in duplicate and albuminuria was determined as the ratio of urinary albumin (µg/ml) to creatinine (mg/ml). For electrophoresis, urine was centrifuged at 17,000 × g for 15 min at 4 °C to remove urinary sediment including whole cells, large membrane fragments, and other debris. 3 µl of the remaining suspension was added to an equal volume of 2 × Laemmli sample buffer and run on a 15% gel under non-reducing conditions. The gel was stained with Coomassie stain (BioRad). BUN was detected with QuantiChrom™ Urea Assay Kit (DIUR-500) (BioAssay Systems, Hayward, CA) according to manufacturer’s instructions. Serum creatinine was detected with (Enzymatic) Reagent Set from Pointe Scientific, Inc. (Canton, MI).
Cheng H., Fan X., Lawson W.E., Paueksakon P, & Harris R.C. (2015). Telomerase deficiency delays renal recovery in mice after ischemia reperfusion injury by impairing autophagy. Kidney international, 88(1), 85-94.
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2

Murine Microalbuminuria and Kidney Health

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A murine microalbuminuria ELISA kit, AlbuwellM™ (Exocell Inc., Philadelphia, PA, USA) was used to detect proteinuria levels. BUN was tested using the QuantiChrom™ Urea Assay Kit (DIUR-500) (BioAssay Systems, Hayward, CA, USA) in line with the manufacturer’s instructions. Serum Cr was measured using the (Enzymatic) Reagent Set from Pointe Scientific, Inc. (Canton, MI, USA).
Tian Y., Shu J., Huang R., Chu X, & Mei X. (2020). Protective effect of renal ischemic postconditioning in renal ischemic-reperfusion injury. Translational Andrology and Urology, 9(3), 1356-1365.
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3

Blood Creatinine and BUN Analysis

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Blood samples were collected every 3 days following treatment and at sacrifice. Blood was collected in heparinized tubes and centrifuged at 14,000 × g for 10 min to obtain plasma samples.
Creatinine concentrations were measured using an enzyme-linked immunosorbent assay (ELISA; Stanbio, TX, USA). BUN concentrations were measured using a QuantiChrom Urea Assay Kit (DIUR-500; BioAssay Systems, Hayward, CA, USA).
Ullah M., Liu D.D., Rai S., Dadhania A., Jonnakuti S., Concepcion W, & Thakor A.S. (2020). Reversing Acute Kidney Injury Using Pulsed Focused Ultrasound and MSC Therapy: A Role for HSP-Mediated PI3K/AKT Signaling. Molecular Therapy. Methods & Clinical Development, 17, 683-694.
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4

Urinary Biomarkers for Kidney Function Assessment

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Urinary albumin levels were determined by ELISA using a murine microalbuminuria ELISA kit, AlbuwellM™ (Exocell Inc., Philadelphia, PA, USA). The urine creatinine concentration was measured with a microplate assay kit, Creatinine Companion (Exocell Inc., Philadelphia, PA, USA). All measurements were performed in duplicate and albuminuria was determined as the ratio of urinary albumin (µg/ml) to creatinine (mg/ml). For electrophoresis, urine was centrifuged at 17,000 × g for 15 min at 4 °C to remove urinary sediment including whole cells, large membrane fragments, and other debris. 3 µl of the remaining suspension was added to an equal volume of 2 × Laemmli sample buffer and run on a 15% gel under non-reducing conditions. The gel was stained with Coomassie stain (BioRad). BUN was detected with QuantiChrom™ Urea Assay Kit (DIUR-500) (BioAssay Systems, Hayward, CA) according to manufacturer’s instructions. Serum creatinine was detected with (Enzymatic) Reagent Set from Pointe Scientific, Inc. (Canton, MI).
Cheng H., Fan X., Lawson W.E., Paueksakon P, & Harris R.C. (2015). Telomerase deficiency delays renal recovery in mice after ischemia reperfusion injury by impairing autophagy. Kidney international, 88(1), 85-94.
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5

Colorimetric Assays for Creatinine and Urea

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Creatinine levels were measured in serum by a quantitative colorimetric method using a QuantiChrom Creatinine Assay Kit (DICT-500), supplied by BioAssay Systems, Hayward, CA, USA, following the manufacturer’s instructions and according to the modified Jaffe method [18 (link)].Urea levels were measured in serum by a quantitative colorimetric method according to [20 (link)] using a QuantiChrom Urea Assay Kit (DIUR-500), supplied by BioAssay Systems, Hayward, CA, USA, according to the manufacturer’s instructions.
Hamed A.E., Khedr S., Ghonamy E., Mahmoud F.A, & Ahmed M.A. (2024). Impact of folic acid supplementation on ischemia‒reperfusion-induced kidney injury in rats: folic acid prophylactic role revisited. The Journal of Physiological Sciences : JPS, 74, 7.
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6

Quantification of Cellular Urea Levels

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Urea concentration was analysed in the supernatant of cell samples with the colorimetric commercial kit QuantiChrom™ Urea Assay Kit-DIUR-500 (BioAssay System, Hayward, CA, USA) according to the manufacturer’s instructions. The 3D-hepatocyte suspension (1 mL) was collected in duplicate and centrifuged to clean off the cells. Supernatants were stored at −20 °C until further analysis. After thawed, samples were vigorously vortexed and 50 µL of each sample was plated in triplicate in a sterile 96 well transparent plate (Nunc™, Thermo Scientific™, Waltham, MA, USA) For posterior analysis, the sterile and supplemented William’s E culture medium absorbance was subtracted from each sample reading.
Rodrigues A.V., Alexandre-Pires G., Valério-Bolas A., Santos-Mateus D., Rafael-Fernandes M., Pereira M.A., Ligeiro D., Nunes T., Alves-Azevedo R., Santos M., Pereira da Fonseca I, & Santos-Gomes G. (2020). 3D-Hepatocyte Culture Applied to Parasitology: Immune Activation of Canine Hepatic Spheroids Exposed to Leishmania infantum. Biomedicines, 8(12), 628.
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7

Serum Urea Measurement in Mice

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Mice were euthanized at the indicated ages. Before removing the kidneys, blood was collected by intracardiac puncture and centrifuged at 2000 x g for serum collection. BUN levels were determined using the QuantiChromTM Urea Assay Kit (DIUR-500) (BioAssay Systems, Hayward, CA).
Zeng F., Miyazawa T., Kloepfer L.A, & Harris R.C. (2014). Deletion of ErbB4 accelerates polycystic kidney disease progression in cpk mice. Kidney international, 86(3), 538-547.
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8

Evaluation of Kidney Injury Biomarkers

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Body weight was measured at week 6 and at sacrifice. Spot urine protein to creatinine ratio (PCR) was assessed at weeks 0, 6 (before LMB2 injection) and at sacrifice by ELISA using the Pierce BCA protein assay kit (Thermo Scientific, IL, USA) and Creatinine assay kit (Abcam, MA, USA), respectively, according to the manufacturer’s instruction. Urinary albumin was determined using Albuwell M kits (Exocell, PA, USA). BUN levels were determined using the QuantiChromTM Urea Assay Kit (DIUR-500) (BioAssay Systems, Hayward, CA, USA).
Tubular injury was monitored by urinary neutrophil gelatinase-associated lipocalin (NGAL) and kidney injury molecule-1 (Kim-1) excretion in spot urine at baseline, week 2 (after DT), 6 (before LMB2) and at sacrifice. NGAL and Kim-1 were measured by ELISA kits (R&D Systems, MN, USA) according to the manufacturer’s instruction. The values were normalized by urinary creatinine concentration.
Lim B.J., Yang J.W., Zou J., Zhong J., Matsusaka T., Pastan I., Zhang M.Z., Harris R.C., Yang H.C, & Fogo A.B. (2017). Tubulointerstitial fibrosis can sensitize the kidney to subsequent glomerular injury. Kidney international, 92(6), 1395-1403.
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9

Serum Urea Measurement in Mice

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Mice were euthanized at the indicated ages. Before removing the kidneys, blood was collected by intracardiac puncture and centrifuged at 2000 x g for serum collection. BUN levels were determined using the QuantiChromTM Urea Assay Kit (DIUR-500) (BioAssay Systems, Hayward, CA).
Zeng F., Miyazawa T., Kloepfer L.A, & Harris R.C. (2014). Deletion of ErbB4 accelerates polycystic kidney disease progression in cpk mice. Kidney international, 86(3), 538-547.
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