Fetal bovine solution

Manufactured by Thermo Fisher Scientific

Sourced in United States

Fetal bovine solution is a cell culture medium supplement derived from the serum of fetal bovine blood. It provides a source of growth factors, vitamins, and other nutrients essential for the cultivation of a variety of cell types in vitro.

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3 protocols using fetal bovine solution

Gastric Cancer Cell Line Cultures

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KRAS wild and mutant GC cell lines were used in this study. Human gastric adenocarcinoma cell line AGS with KRAS mutation (CRL-1739) and the normal human gastric epithelial cell line GES-1 were obtained from the American Type Culture Collection (Manassas, VA, USA). GC cell line SGC-7901 with wild type KRAS was obtained from the National Infrastructure of Cell Line Resource (Beijing, China). All cells were cultured in RPMI 1640 supplemented with 10% fetal bovine solution (GIBCO, Carlsbad, CA, USA) at 37°C with 5% CO₂ supplement.
Vitexin (Selleck Chemicals, Houston, TX, USA) was dissolved in dimethyl sulfoxide (Sigma, St. Louis, MO, USA) to prepare the stock solution (100 mM). Before experiments, the stock solution was diluted with phosphate-buffered saline (PBS) to the desired concentration.

Zhou P., Zheng Z.H., Wan T., Wu J., Liao C.W, & Sun X.J. (2021). Vitexin Inhibits Gastric Cancer Growth and Metastasis through HMGB1-mediated Inactivation of the PI3K/AKT/mTOR/HIF-1α Signaling Pathway. Journal of Gastric Cancer, 21(4), 439-456.

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Isolation and Culture of Rat Cortical Neurons

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In the study, Sprague Dawley (a newborn) rat that had not completed 24 h was used to obtain cortex neurons. Briefly, after the rats were decapitated quickly, the removed cortices were transferred to 5 mL of phosphate buffer solution (PBS), and macro‐fragmentation was performed with the help of a scalpel and then micro‐fragmentation was performed with Trypsin (0.02%). The cells were then centrifuged at 200g for 5 min. Cells sinking to the bottom are cellular medium, 88% NBM (Neurobasal medium, Gibco, USA), 10% FBS (Fetal bovine solution, Gibco, USA), 2% B‐27% (Supplement, Thermo Fisher, Germany), 0.1% antibiotic (Penicillin–Streptomycin) and amphotericin B (Thermo Fisher, Germany) were added. For 10 days, the cells were incubated at 5% CO₂ and 37°C, changing the medium every 3 days.
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Taghizadehghalehjoughi A., Naldan M.E., Yeni Y., Genc S., Hacimuftuoglu A., Isik M., Necip A., Bolat İ., Yildirim S., Beydemir S, & Baykan M. (2024). Effect of fentanyl and remifentanil on neuron damage and oxidative stress during induction neurotoxicity. Journal of Cellular and Molecular Medicine, 28(4), e18118.

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Synthesizing and Utilizing JBNTs in hMSC Research

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JBNTs were synthesized by an effective approach published previously (Chen, Yu, & Chen, 2017 ). Human MSCs (hMSCs) (containing ≥750,000 cells/vial), Stem Cell Growth Medium BulletKit, and Tritcphalloidin were purchased from Lonza. Trypsin–EDTA solution (Gibco, 0.25%), phosphate buffered saline (PBS, Gibco), GlutaMAX media (Gibco), fetal bovine Solution (FBS, Gibco), Penicillin–Streptomycin (Gibco, 10,000 U/ml), and Triton X-100 (Invitrogen, 1.0%) were purchased from Thermo Fisher. Formalin and Ethanol (70% solution) were obtained from Fisher Scientific. Then, 12-well, 24-well, and 96-well clear round-bottom microplates were obtained from Corning. The catalog numbers of these plates are 353043, 353047, and 353,072, respectively. The 8-μm cell insert also was obtained from corning, the catalog number is 353182. The 8-well cell culture slides were purchased from Thermo Fisher, and the catalog number is 155409. FN (1.0 mg/ml) came from Gibco, and its catalog number is PHE0023.

Zhou L., Yau A., Yu H., Kuhn L., Guo W, & Chen Y. (2020). Self-assembled biomimetic Nano-Matrix for stem cell anchorage. Journal of biomedical materials research. Part A, 108(4), 984-991.

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