All reagents were of analytical purity. CS, with a viscometric average molecular weight of 467 kDa and 15% acetylated units, and glutaraldehyde (GA), as an aqueous solution with a concentration of 25%, were purchased from Sigma-Aldrich Chemie GmbH, Darmstadt, Germany. Fastac 10EC (BASF Agro B.V., Arnhem, The Netherlands) is commercially available in vials of 2 mL solution (α-cypermethrin: 100 g L−1). Synthetic emulsions of Fastac 10EC were prepared in deionized Milli-Q water (Milli-Q PF, Millipore, Schaffhausen, Switzerland) and sonicated for 15 min using a VCX 750 ultrasonicator (Sonics & Materials Inc., Newtown, CT, USA). Poly(methylmethacrylate) (PMMA) particles with a mesh size of about 32–50 μm were used as porogen and were prepared via free radical polymerization according to a procedure previously reported by Pradny et al. [30 (link)]. The chemical structures of materials used in this study are presented in Figure 1 .
Milli q pf
Manufactured by Merck Group
Sourced in Switzerland
The Milli-Q PF is a water purification system designed to produce high-purity water for use in various laboratory applications. It utilizes a combination of advanced filtration and purification technologies to remove impurities and contaminants from the water supply, resulting in ultrapure water that meets the highest standards of purity.
Automatically generated - may contain errors
Lab products found in correlation
Glutaraldehyde ga, by Merck Group (1 mentions)
Vcx 750, by Sonics (1 mentions)
6545 accurate mass q tof lc ms system, by Agilent Technologies (1 mentions)
Gx 281, by Waters Corporation (1 mentions)
Cary 50 bio uv vis spectrophotometer, by Agilent Technologies (1 mentions)
Prep lc system, by Waters Corporation (1 mentions)
5 mm cpp tci cryo probe, by Bruker (1 mentions)
Hplc purificationsystem, by Gilson (1 mentions)
Avance 3 hd spectrometer, by Bruker (1 mentions)
600 pump, by Waters Corporation (1 mentions)
Fc204 fraction collector, by Gilson (1 mentions)
Onyx monolithic c18 column, by Phenomenex (1 mentions)
600 mhz spectrometer, by Agilent Technologies (1 mentions)
Luna c18 column, by Phenomenex (1 mentions)
V 650 uv vis spectrophotometer, by Jasco (1 mentions)
4 protocols using milli q pf
1
Synthesis and Characterization of CS-GA Hydrogels
2
Comprehensive Spectroscopic Characterization of Compounds
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IR spectra
were recorded as a dry film on a Bruker Alpha II spectrometer or a
Bruker Invenio S spectrometer equipped with an HTS-XT accessory. UV
spectra were recorded as methanol solutions on a Varian Cary 50-Bio
UV/vis spectrophotometer. NMR spectra were recorded at 25 °C
on a 600 MHz Bruker Avance III HD spectrometer, equipped with a triple
resonance 5 mm CPP TCI cryo-probe, and operating at a frequency of
600.0 MHz for the 1H nucleus and 150.9 MHz for the 13C nucleus. All 2D NMR experiments were acquired with non-uniform
sampling (NUS) set to 40% (for 1H–1H
detected experiments) or 35% (for 1H–13C detected experiments). 1H and 13C NMR chemical
shifts were referenced to the solvent peak for DMSO-d6 at δH 2.50 δC 39.50.
NMR FID processing and data interpretation was done using MestReNova
software, version 14.2. High-resolution mass spectra were recorded
on an Agilent 6545 Accurate-Mass Q-TOF LC/MS system (1290 Infinity
II) equipped with a dual AJS ESI source. Semi-preparative scale HPLC
purification was performed with either a Gilson HPLC purification
system equipped with a GX-281 liquid handler, a 322-binary pump, and
a 172-photodiode array detector or a Waters Prep LC system, equipped
with a Delta 600 pump and a 996-photodiode array detector. All solvents
used for chromatography, UV, and MS were HPLC grade, and the H2O was Millipore Milli-Q PF filtered.
were recorded as a dry film on a Bruker Alpha II spectrometer or a
Bruker Invenio S spectrometer equipped with an HTS-XT accessory. UV
spectra were recorded as methanol solutions on a Varian Cary 50-Bio
UV/vis spectrophotometer. NMR spectra were recorded at 25 °C
on a 600 MHz Bruker Avance III HD spectrometer, equipped with a triple
resonance 5 mm CPP TCI cryo-probe, and operating at a frequency of
600.0 MHz for the 1H nucleus and 150.9 MHz for the 13C nucleus. All 2D NMR experiments were acquired with non-uniform
sampling (NUS) set to 40% (for 1H–1H
detected experiments) or 35% (for 1H–13C detected experiments). 1H and 13C NMR chemical
shifts were referenced to the solvent peak for DMSO-d6 at δH 2.50 δC 39.50.
NMR FID processing and data interpretation was done using MestReNova
software, version 14.2. High-resolution mass spectra were recorded
on an Agilent 6545 Accurate-Mass Q-TOF LC/MS system (1290 Infinity
II) equipped with a dual AJS ESI source. Semi-preparative scale HPLC
purification was performed with either a Gilson HPLC purification
system equipped with a GX-281 liquid handler, a 322-binary pump, and
a 172-photodiode array detector or a Waters Prep LC system, equipped
with a Delta 600 pump and a 996-photodiode array detector. All solvents
used for chromatography, UV, and MS were HPLC grade, and the H2O was Millipore Milli-Q PF filtered.
3
NMR and HPLC Characterization of Compounds
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NMR spectra were recorded in DMSO-d6 (δH 2.50 and δC 39.5) at 25 °C on a Bruker Avance HDX 800 MHz spectrometer equipped with a TCI cryoprobe. The HPLC system included a Waters 600 pump fitted with a 996 photodiode array detector and Gilson FC204 fraction collector. All solvents used for extraction and chromatography were Lab-Scan HPLC grade, and the H2O was Millipore Milli-Q PF filtered.
4
Spectroscopic Characterization Protocol
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General experimental procedures: UV spectra were acquired on a Jasco V650 UV/vis spectrophotometer. NMR spectra were recorded at 30ºC on Varian 600 MHz spectrometer. LC-MS spectra were obtained using a Waters ZQ electrospray mass spectrometer with a Phenomenex Luna C 18 column (4.6 50 mm, 3 m). Analytical HPLC was performed with a Phenomenex Onyx Monolithic C 18 column (4.6 x 100 mm). A Betasil C 18 column (21.2 150 mm) was used for semi-preparative HPLC separation. All HPLC and LC-MS experiments were performed with MeOH-H 2 O gradient solvent system. Milipore Milli-Q PF filtered H 2 O and HPLC grade solvents were used for chromatography.
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