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Xanthine oxidase assay kit

Manufactured by Abcam
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The Xanthine Oxidase Assay Kit is a colorimetric assay that quantifies the activity of the enzyme xanthine oxidase. Xanthine oxidase catalyzes the oxidation of xanthine to uric acid, which is measured by the kit. The assay provides a simple, accurate, and high-throughput method for determining xanthine oxidase activity in various biological samples.

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Lab products found in correlation

Echomri 500, by Echo Medical Systems (1 mentions) 2 4 6 tris 2 pyridyl s triazine, by Merck Group (1 mentions) Folin denis reagent, by Merck Group (1 mentions) 2 2 azinibis 3 ethyl benzothiazoline 6 sulfonic acid, by Merck Group (1 mentions) Penicillin streptomycin p s, by Thermo Fisher Scientific (1 mentions) Trypsin edta, by Thermo Fisher Scientific (1 mentions) Gallic acid, by Merck Group (1 mentions) Quercetin, by Merck Group (1 mentions) Acetic acid, by Merck Group (1 mentions) Lipopolysaccharide, by Merck Group (1 mentions) Iron 3 chloride hexahydrate, by Merck Group (1 mentions)

5 protocols using xanthine oxidase assay kit

1

Artemether's Effect on Moco Synthase

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αTC1 cells were pretreated with artemether for three days and lysed with NP-40 buffer. The measurement of Moco synthase was performed with Xanthine Oxidase Assay Kit (Abcam ab102522).
Li J., Casteels T., Frogne T., Ingvorsen C., Honoré C., Courtney M., Huber K.V., Schmitner N., Kimmel R.A., Romanov R.A., Sturtzel C., Lardeau C.H., Klughammer J., Farlik M., Sdelci S., Vieira A., Avolio F., Briand F., Baburin I., Májek P., Pauler F.M., Penz T., Stukalov A., Gridling M., Parapatics K., Barbieux C., Berishvili E., Spittler A., Colinge J., Bennett K.L., Hering S., Sulpice T., Bock C., Distel M., Harkany T., Meyer D., Superti-Furga G., Collombat P., Hecksher-Sørensen J, & Kubicek S. (2017). Artemisinins Target GABAA Receptor Signaling and Impair α Cell Identity. Cell, 168(1-2), 86-100.e15.
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2

Body Composition and Metabolic Analysis in Mice

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After 16 weeks of feeding, mice underwent body composition analysis for whole body fat mass, lean mass and total body water utilizing an EchoMRI-500 for quantitative magnetic resonance analysis (Echo Medical Systems, Houston, TX, USA) by a method established in our laboratory.22 (link), 23 (link) Venous blood samples were collected from a subset of fasting mice in each treatment group and plasma was stored at −80°C for glucose and insulin assay and homeostatic model assessment of insulin resistance (HOMA-IR) as previously described.17 , 23 (link) Cardiac xanthine oxidase activity was determined in tissue protein supernatant using a xanthine oxidase assay kit (Abcam, Cambridge, MA).
Jia G., Habibi J., Bostick B.P., Ma L., DeMarco V.G., Aroor A.R., Hayden M.R., Whaley-Connell A.T, & Sowers J.R. (2014). Uric Acid Promotes Left Ventricular Diastolic Dysfunction in Mice Fed a Western Diet. Hypertension, 65(3), 531-539.
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3

Xanthine Oxidase Inhibitory Assay for Centella officinalis

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XO inhibitory activity was assayed using a commercial xanthine oxidase assay kit (Abcam, Cambridge, MA, USA) according to the manufacturer’s instructions. Briefly, RAW 264.7 cells were plated at 5 × 105 cells/well. After 4 h, the cells were treated with 10, 50, and 100 μg/mL of C. officianalis for 24 h. The treated cell pellets were mixed with assay buffer and the supernatants were isolated. The working solutions were added to the supernatants and incubated at 37 °C. After 1 h, the absorbance was measured at 570 nm.
Hwang K.A., Hwang Y.J, & Song J. (2016). Antioxidant activities and oxidative stress inhibitory effects of ethanol extracts from Cornus officinalis on raw 264.7 cells. BMC Complementary and Alternative Medicine, 16, 196.
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4

Measuring Xanthine Oxidase and NADPH Oxidase

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Xanthine oxidase (XO) assay kit was purchased from Abcam, Cambridge, MA, and the assay was performed per the protocol provided by the vendor. NADPH (nicotinamide adenine dinucleotide phosphate, reduced form) oxidase assay was performed by the University of Michigan Metabolomics Core.39
Avula U.M., Hernandez J.J., Yamazaki M., Valdivia C.R., Chu A., Rojas-Pena A., Kaur K., Ramos-Mondragón R., Anumonwo J.M., Nattel S., Valdivia H.H, & Kalifa J. (2018). Atrial Infarction-Induced Spontaneous Focal Discharges and Atrial Fibrillation in Sheep: Role of Dantrolene-Sensitive Aberrant Ryanodine Receptor Calcium Release. Circulation. Arrhythmia and electrophysiology, 11(3), e005659.
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5

Antioxidant and Anti-inflammatory Assays

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Folin-Denis reagent, sodium carbonate, aluminum chloride, potassium acetate, potassium persulfate, 1,1-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azinibis 3-ethyl benzothiazoline-6-sulfonic acid (ABTS), 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), iron(III) chloride hexahydrate, gallic acid, acetic acid, lipopolysaccharide (LPS), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), ascorbic acid (Vit C), and quercetin were purchased from Sigma-Aldrich (St. Louis, MO, USA). Iron (II) sulfate heptahydrate (FeSO4) was purchased from Junsei (Tokyo, Japan). Dulbecco’s Modified Eagle’s Medium (DMEM), fetal bovine serum (FBS), phosphate-buffered saline (PBS), penicillin-streptomycin (P/S), and trypsin-EDTA were obtained from Gibco (Waltham, MA, USA). The xanthine oxidase (XO) assay kit was purchased from Abcam (Cambridge, MA, USA). The other reagents used were of analytical grade.
Hwang K.A., Hwang Y.J, & Song J. (2016). Antioxidant activities and oxidative stress inhibitory effects of ethanol extracts from Cornus officinalis on raw 264.7 cells. BMC Complementary and Alternative Medicine, 16, 196.
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