N2a cells

Manufactured by Thermo Fisher Scientific

Sourced in United States

N2a cells are a mouse neuroblastoma cell line commonly used in neuroscience research. They are derived from a spontaneous tumor in the body of an albino mouse and can be differentiated into neuron-like cells. The N2a cell line is a widely used model system for studying various aspects of neuron biology, including neurotransmitter signaling, neuronal development, and neurodegenerative processes.

Automatically generated - may contain errors

Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (6 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (3 mentions) Horse serum, by Thermo Fisher Scientific (2 mentions) C17.2 cells, by Thermo Fisher Scientific (2 mentions) Mc170hd microsystem, by Leica (2 mentions) N2a cells, by American Type Culture Collection (2 mentions) Dmem medium, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Triacsin c, by Merck Group (1 mentions) Penicillin streptomycin, by PAN Biotech (1 mentions) Fetal bovine serum (fbs), by PAN Biotech (1 mentions) Mouse n2a cells, by American Type Culture Collection (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Hek293t cell, by American Type Culture Collection (1 mentions)

Spelling variants of this product

N2a neuroblastoma cells (3 citations)

8 protocols using n2a cells

N2a Cell Culture and PrP106-126 Aggregation

Check if the same lab product or an alternative is used in the 5 most similar protocols

N2a cells were purchased from Cell Resource Center (IBMS, CAMS/PUMC, China), and tested to be mycoplasma free. N2a cells were cultured in Gibco DMEM (catalog no. C11995500BT) supplemented with 10% (v/v) fetal bovine serum (Gibco, NY, USA) at 37 °C with 5% CO₂ in a humid incubator.
We obtained synthesized PrP106-126 peptide (KTNMKHMAGAAAAGAVVGGLG; > 98% purity) from YaMei Peptides Bio-Tech. It was dissolved in PBS to a 1 mM concentration and shaken to aggregate at 4 °C for 24 h. Experiments were conducted with a final peptide concentration of 100 μM.

Li J., Lai M., Zhang X., Li Z., Yang D., Zhao M., Wang D., Sun Z., Ehsan S., Li W., Gao H., Zhao D, & Yang L. (2022). PINK1-parkin-mediated neuronal mitophagy deficiency in prion disease. Cell Death & Disease, 13(2), 162.

+ Open protocol

+ Expand

Neuro-2a Cell Culture and Mouse Experiment

Check if the same lab product or an alternative is used in the 5 most similar protocols

C57BL/6 mice were purchased from the Peking University Health Science Center Department of Laboratory Animal Science. All experiments in this study used 8-10-week-old male mice. The mice were housed under a 12-h light-dark cycle in a thermostatically controlled barrier facility with free access to food. Mice were deeply anesthetized with isoflurane and then sacrificed. For all experiments, a minimal number of animals were used, and the pain was minimized to the best extent possible. This study was approved by the Local Ethics Committee for Animal Research Studies at the Peking University Health Science Center, No. LA2017100.
Neuro-2a (N2A) cells were purchased from Procell (Wuhan, China) and were maintained and stored in accordance with the instructions provided by the supplier. Briefly, N2A cells were maintained in DMEM supplemented with 10% fetal bovine serum (10100139C, Gibco, California, US) and 1% penicillin and streptomycin. The cells were cultured in a humidified incubator containing 5% CO2 at 37 °C.

Cheng Q., Wang J., Li M., Fang J., Ding H., Meng J., Zhang J., Fang X., Liu H., Ma C., Chen C, & Zhang W. (2022). CircSV2b participates in oxidative stress regulation through miR-5107-5p-Foxk1-Akt1 axis in Parkinson's disease. Redox Biology, 56, 102430.

+ Open protocol

+ Expand

Neuroblastoma and Neural Progenitor Cell Cultures

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse neuroblastoma cells (N2a cells) were purchased from ATCC (American Type Culture Collection), and mouse neural progenitor cells (C17.2 cells) were purchased from ECACC (European Collection of Cell Culture). N2a cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (Cat#10099141, Gibco, Thermo Fisher Scientific, California, USA), 100 U/ml penicillin and 100 μg/ml streptomycin at 37 °C in a humidified atmosphere of 5% CO₂. C17.2 cells were cultured as described for the N2a cell protocol except that 5% horse serum was added to the medium (Cat#26050070, Gibco). The two cell lines were grown to 80% confluence, and then passaged by trypsin at a ratio of 1:4. The cell morphology of duloxetine-induced cytotoxicity and neurite outgrowth was recorded using a living-cell imaging system: a Leica MC170HD microsystem (Leica Microsystems, Wetzlar, Germany).

Gao W., Chen R., Xie N., Tang D., Zhou B, & Wang D. (2020). Duloxetine-Induced Neural Cell Death and Promoted Neurite Outgrowth in N2a Cells. Neurotoxicity Research, 38(4), 859-870.

+ Open protocol

+ Expand

Oxygen-glucose deprivation and reoxygenation of N2a cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell culture and Oxygen-glucose deprivation/reoxygenation (OGD/R) N2a cells were purchased from the National Collection of Authenticated Cell Culture (https://cellbank.org.cn). N2a cells were grown in DMEM (Gibco) containing 10% fetal bovine serum (BI) and cultured in an incubator (37°C, and 5% CO2). OGD/R model was carried out as previously described with modi cation [19] . Brie y, For the induction of oxygen-glucose deprivation (OGD), cells were washed with PBS and maintained within the glucose-free DMEM medium (Gibco) in the incubator containing 95% N2 and 5% CO2 for different times (2h, 3h, and 4h) at 37°C. For reoxygenation, cells were then incubated again in a normoxic incubator with fresh DMEM containing 10% fetal bovine serum for 24h. The cells that were not exposed to OGD/R were de ned as control group.

Liu Y., Gui Y., Tang H., Yu J., Yuan Z., Liu L., Ma X, & Li C. (2023). DDX3X Deficiency Attenuates Pyroptosis Induced by Oxygen-glucose Deprivation/Reoxygenation in N2a Cells. Current neurovascular research, 20(2).

+ Open protocol

+ Expand

Evaluating Neuroprotective Effects of Triacsin C

Check if the same lab product or an alternative is used in the 5 most similar protocols

N27 cells (Merck, Bayswater, Australia) were cultured in RPMI 1640 (Gibco, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (Gibco, Thermo Fisher Scientific) and 1% penicillin-streptomycin in a humid incubator at 37 °C with 5% CO 2 . N2a cells (National Collection of Authenticated Cell Cultures) were cultured in DMEM (Gibco, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (Gibco, Thermo Fisher Scientific) and 1% penicillin-streptomycin in a humid incubator at 37 °C with 5% CO 2 . Cells were seeded onto 96-well plates and then treated with Triacsin C, DMSO, or MPP + (Sigma, D048) after plating. Specifically, N27 or N2a cells were pretreated with 3 or 10 μM Triacsin C or 0.0125% DMSO for 48 h and then treated with 0.5-5 mM MPP + for 24 h. The ACSL4-KD or WT N27 and N2a cells were treated with 0.5-7.5 mM MPP + . The ACSL1-KD or WT N27 and N2a cells were treated with 0.1-7.5 mM MPP + . Then, cell viability was assessed using an MTT cytotoxicity assay kit (Sigma, M2003) as previously described [9] (link).

Tang F., Zhou L.Y., Li P., Jiao L.L., Chen K., Guo Y.J., Ding X.L., He S.Y., Dong B., Xu R.X., Xiong H, & Lei P. (2023). Inhibition of ACSL4 Alleviates Parkinsonism Phenotypes by Reduction of Lipid Reactive Oxygen Species. Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics, 20(4).

+ Open protocol

+ Expand

Neuroblastoma and Neural Progenitor Cell Cultures

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse neuroblastoma cells (N2a cells) were purchased from ATCC (American Type Culture Collection), and mouse neural progenitor cells (C17.2 cells) were purchased from ECACC (European Collection of Cell Culture). N2a cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (Cat#10099141, Gibco, Thermo Fisher Scientific, California, USA), 100 U/ml penicillin and 100 μg/ml streptomycin at 37°C in a humidified atmosphere of 5% CO 2 . C17.2 cells were cultured as described for the N2a cell protocol except that 5% horse serum was added to the medium (Cat#26050070, Gibco). The two cell lines were grown to 80% confluence, and then passaged by trypsin at a ratio of 1:4. The cell morphology of duloxetine-induced cytotoxicity and neurite outgrowth was recorded using a living-cell imaging system: a Leica MC170HD microsystem (Leica Microsystems, Wetzlar, Germany).

Gao W., Chen R., Xie N., Tang D., Zhou B., & Wang D. (2020). Duloxetine induced neural cell death and promoted neurite outgrowth in N2a cells.

+ Open protocol

+ Expand

Culturing Mouse N2A Neuroblastoma Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse N2A cells were purchased from American Type Culture Collection (ATCC). N2A cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM), low glucose, GlutaMAX^TM, pyruvate (21885025, Thermo Fisher) supplemented with 10 percent fetal bovine serum (FBS; P30-1985, Pan Biotech) and 100 U/ml penicillin-streptomycin (P0607300, PAN Biotech) at 37 °C in a humidified atmosphere of 95 percent air and 5 percent CO₂.

Beker M.C., Caglayan B., Caglayan A.B., Kelestemur T., Yalcin E., Caglayan A., Kilic U., Baykal A.T., Reiter R.J, & Kilic E. (2019). Interaction of melatonin and Bmal1 in the regulation of PI3K/AKT pathway components and cellular survival. Scientific Reports, 9, 19082.

+ Open protocol

+ Expand

Culturing HEK293T and N2a Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

HEK293T cells (ATCC) and N2a cells (Thermo Fisher) were maintained in Dulbecco’s modified Eagle medium supplemented with 10% fetal bovine serum (Invitrogen) and 1% penicillin/streptomycin (at 10,000 U/mL, Thermo Fisher). The cells were cultured at an 80% confluency under 37 °C in a humidified atmosphere containing 5% CO₂.

Chen X., He X., Yang Y.Y, & Wang Y. (2022). Amyotrophic Lateral Sclerosis-Associated Mutants of SOD1 Modulate miRNA Biogenesis through Aberrant Interactions with Exportin 5. ACS chemical biology, 17(12), 3450-3457.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!