Il 12p40

All commercially available compounds were used without further purification unless otherwise noted. Cetyltrimethylammonium chloride (CTAB), tetraethyl orthosilicate (TEOS), 1,4-bis(triethoxysilyl)benzene (BTEB), Sodium hydroxide (NaOH) and FITC-labelled dextrans (10 kDa) were purchased from Sigma Aldrich. 4,6-diamidino-2-phenylindole (DAPI) and Hoechst 33342 were purchased from Invitrogen. RPMI1640 cell culture medium was from Gibco. IL-12p40 and IFN-γ ELISA kits were purchased from Abcam. Ultrapure water (18.2 MΩ; Millpore Co., USA) was used throughout the experiment.

Western blot was done as described in Ref.^{11 (link)}. Briefly, HUVECs or THP-1 cells were lysed in Radio immunoprecipitation assay buffer with protease inhibitor cocktail (Roche). After measuring the protein concentration, 50 μg protein was used to electrophoresed in sodium dodecyl sulfate–polyacrylamide gel, and transfer it to PDVF membrane (Amersham Biosciences). The transferred membrane was sealed with 5% BSA at room temperature, and incubated with primary antibody at 4 °C overnight. Then the membrane was washed with Tris-buffered saline-Tween 20 solution, and incubated with secondary antibody at room temperature for 1 h. The band was visualized with imaging system after washing. The following antibodies were used in this study: Ang1 (Proteintech); p65 (AFFINITY); p-p65 (CST); GAPDH (AtaGenix); IL-1β (Proteintech); TNF-α (Proteintech); IL-6 (Proteintech); IL12p40 (abcam).