Oligonucleotide primers

All experimental procedures for RNA extraction used in this study were in accordance with those employed in previous studies [27 (link)]. Total RNA (n = 5) was extracted using RNA Extraction Reagent (Servicebio, Wuhan, China) and converted to complementary DNA (cDNA) using the Servicebio RT First Strand cDNA Synthesis Kit (Servicebio). qPCR analysis was performed using 2× SYBR Green qPCR Master Mix (Servicebio). The mRNA-expression levels of the target genes were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA-expression levels and fold-changes in expression differences were calculated using the 2^−ΔΔCT method [28 (link)]. The sequences of the oligonucleotide primers (Servicebio) used in this study are shown in Table 1.

RNA extraction was conducted according to the procedures described previously [30 (link)]. After the extraction with RNA extraction reagent (Servicebio, Wuhan, China), the total RNA was used to synthesize complementary DNA using the Servicebio^®RT First Strand cDNA Synthesis Kit (Servicebio, Wuhan, China). Quantitative PCR was performed using 2×SYBR Green qPCR Master Mix (Servicebio, Wuhan, China). β-actin mRNA levels were used to normalize the mRNA levels of genes selected and the 2^−ΔΔCT method was utilized to calculate the fold-change in expression. Table 1 contains a list of all oligonucleotide primers (Servicebio, Wuhan, China) in the experiment.