The correlation of miR-10a and PIK3CA was predicted by RegRNA 2.0. miR-10a mimics or control scrambled sequences were transfected into HK-2 cells for 48 h using Lipofectamine 2000 at a 2:1 M ratio. The activity of luciferase in cells was tested by the dual-luciferase assay (Solarbio, Wuhan, China).
Dual luciferase assay
Manufactured by Solarbio
The Dual-luciferase assay is a laboratory technique used to measure the activities of two different luciferase reporter enzymes simultaneously within a single sample. It provides a quantitative analysis of gene expression and is commonly used in cell-based assays to study transcriptional regulation and protein function.
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Lab products found in correlation
2 protocols using dual luciferase assay
1
Validating miR-10a-PIK3CA Interaction
2
miR-10a Regulates PIK3CA in HK-2 Cells
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The correction of miR-10a and PIK3CA was predicted RegRNA 2.0. miR-10a mimics or control scrambled were transfected into HK-2 cells for 48 h using Lipofectamine 2000 at a 2:1 molar ratio. The activity of cells luciferase was tested by dual-luciferase assay (Solarbio, Wuhan, China).
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