The high-performance liquid chromatography (HPLC)-photodiode array (PDA)-MS system consisted of a surveyor MS HPLC pump (Thermo Fisher Scientific Inc, Waltham, Massachusetts), HTC PAL autosampler with cooled stack (CTC Analytics AG, Zwingen, Switzerland) and a
surveyor PDA detector (Thermo Fisher Scientific Inc) equipped with a linear trap quadrupole linear ion-trap LC-MS (Thermo Fisher Scientific Inc). Chromatographic separations were achieved by gradient elution using a Waters symmetry C 18 (2.1 mm  50 mm, 3.5 mm particle size) column which was operated at room temperature. Data were acquired and interpreted with
Xcalibur software version 2.0 (Thermo Fisher Scientific Inc). For the in vitro experiments, the concentrations of the test compounds CPX, CPZ, DIC, LVX, 8-MOP, and TIM were measured using HPLC. Standard calibration curves of the test compounds were always included during measurements. More details about the analytical conditions that were applied and the calibration range of the analytical methods can be found in supporting information.
Reinen J., van Sas P., van Huygevoort T., Rubio L., Scase K, & Wenker M. (2018). Development of a Phototoxicity Testing Strategy for Accurate Photosafety Evaluation of Pharmaceuticals Based on the Assessment of Possible Melanin-Binding Effects. International journal of toxicology, 37(4).