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Alexa fluor 568 labeled goat anti rabbit

Manufactured by Thermo Fisher Scientific
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Alexa Fluor 568-labeled goat anti-rabbit is a fluorescently-labeled secondary antibody used in a variety of immunochemical techniques. It is designed to bind to rabbit primary antibodies, allowing for the detection and visualization of target proteins or other biomolecules.

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Lab products found in correlation

Alexa fluor 488 labeled goat anti mouse, by Thermo Fisher Scientific (3 mentions) Halotag tmr ligand, by Promega (2 mentions) Axio observer d1 microscope, by Zeiss (2 mentions) Alpha plan apochromat 100 1.40 oil immersion objective, by Zeiss (2 mentions) Brightline single band laser filter set, by IDEX Corporation (2 mentions) β actin, by Abcam (1 mentions)

Close spelling variants of this product

Alexa Fluor 568-labeled goat anti-rabbit IgG Alexa Fluor 488-or Alexa Fluor 568-labeled goat anti-rabbit antibodies Alexa Fluor 568 labeled goat anti-rabbit IgG secondary antibody Alexa Fluor 568 goat anti-rabbit Anti-goat Alexa-Fluor 568 Alexa Fluor 568 anti-rabbit Alexa 568 goat anti-rabbit Alexa Fluor 568 goat Alexa Fluor rabbit 568 Alexa Fluor goat anti-rabbit

3 protocols using alexa fluor 568 labeled goat anti rabbit

1

Immunoblotting and Immunofluorescence for HCV

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Primary antibodies used were against HCV core (Institute of Immunology, Tokyo, Japan), HCV NS5A (Virogen, Watertown, MA), β-Actin (Abcam, Cambridge, UK), Nrf2 (Santa Cruz, Dallas, TX), and p-Nrf2, phospho-serine 40, (Abcam). For immunoblot analysis, HRP-labeled secondary antibodies against mouse IgG, rabbit IgG, and goat IgG (GE Healthcare) were used depending on the primary antibodies used for immunoblotting. For immunofluorescence staining, Alexa-fluor-488-labeled goat anti-mouse and Alexa-fluor-568-labeled goat anti-rabbit (Life Technologies) secondary antibodies were used.
Murakami Y., Sugiyama K., Ebinuma H., Nakamoto N., Ojiro K., Chu P.S., Taniki N., Saito Y., Teratani T., Koda Y., Suzuki T., Saito K., Fukasawa M., Ikeda M., Kato N., Kanai T, & Saito H. (2018). Dual effects of the Nrf2 inhibitor for inhibition of hepatitis C virus and hepatic cancer cells. BMC Cancer, 18, 680.
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2

Confocal Microscopy of Condensate Formation

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An Axio Observer D1 Microscope (Zeiss, Germany) equipped with an Alpha Plan-Apochromat 100 × /1.40 Oil immersion Objective (Zeiss, Germany) and an Evolve 512 × 512 EMCCD camera with pixel size 16.0 μm (Photometrics; Tucson, AZ) was used for in vitro condensate formation, immunofluorescence, and live-cell imaging. A Brightline® single-band laser filter set (Semrock; excitation filter: FF01–561/14, emission filter: FF01–609/54, and dichroic mirror: Di02-R561–25 × 36) was used for the excitation and emission of HaloTag® TMR ligand (Promega; G8251) or Alexa Fluor 568-labeled goat anti-rabbit (Life Technologies; A11011). A Brightline® single-band laser filter set (Semrock; excitation filter: FF02–482/18–25, emission filter: FF01–525/45–25, and dichroic mirror: Di02-R488–25 × 36) was used for the excitation and emission of Alexa Fluor 488-labeled goat anti-mouse (Life Technologies; A11029). The microscope and EMCCD camera were controlled by the computer via SlideBook 6.0 software (3i). Images were processed and presented using Adobe PhotoShop (Adobe Inc).
Kent S., Brown K., Yang C.H., Alsaihati N., Tian C., Wang H, & Ren X. (2020). Phase-Separated Transcriptional Condensates Accelerate Target-Search Process Revealed by Live-Cell Single-Molecule Imaging. Cell reports, 33(2), 108248.
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3

Confocal Microscopy of Condensate Formation

Check if the same lab product or an alternative is used in the 5 most similar protocols
An Axio Observer D1 Microscope (Zeiss, Germany) equipped with an Alpha Plan-Apochromat 100 × /1.40 Oil immersion Objective (Zeiss, Germany) and an Evolve 512 × 512 EMCCD camera with pixel size 16.0 μm (Photometrics; Tucson, AZ) was used for in vitro condensate formation, immunofluorescence, and live-cell imaging. A Brightline® single-band laser filter set (Semrock; excitation filter: FF01–561/14, emission filter: FF01–609/54, and dichroic mirror: Di02-R561–25 × 36) was used for the excitation and emission of HaloTag® TMR ligand (Promega; G8251) or Alexa Fluor 568-labeled goat anti-rabbit (Life Technologies; A11011). A Brightline® single-band laser filter set (Semrock; excitation filter: FF02–482/18–25, emission filter: FF01–525/45–25, and dichroic mirror: Di02-R488–25 × 36) was used for the excitation and emission of Alexa Fluor 488-labeled goat anti-mouse (Life Technologies; A11029). The microscope and EMCCD camera were controlled by the computer via SlideBook 6.0 software (3i). Images were processed and presented using Adobe PhotoShop (Adobe Inc).
Kent S., Brown K., Yang C.H., Alsaihati N., Tian C., Wang H, & Ren X. (2020). Phase-Separated Transcriptional Condensates Accelerate Target-Search Process Revealed by Live-Cell Single-Molecule Imaging. Cell reports, 33(2), 108248.
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