D maltose monohydrate

The E. coli strains and their mutants used in this study are listed in Table 1. Whole-genome sequences of the 442 O121:H19 strains (Data set S1) previously used in our phylogenetic analysis (13 ) were used for the analysis of IS insertion into the lacZ and iee genes.
Bacteria were grown in the following media: LB (1% [wt/vol] Bacto Tryptone, Gibco; 0.5% [wt/vol] Bacto Yeast Extract, Becton, Dickinson [BD]; 1% [wt/vol] sodium chloride, nacalai tesque), LB agar (LB containing 1.5% [wt/vol] Bacto Agar, BD), MAC (Difco MacConkey agar base, BD; 1% [wt/vol] lactose monohydrate, Wako), MAC not supplemented with lactose (Difco MacConkey agar base), Pearlcore MAC (Pearlcore MacConkey agar, Eiken Chemical Co.), MM (Difco M9 Minimal Salts, BD; 2 mM magnesium sulfate heptahydrate, Wako; 0.1% D-[+]-glucose, nacalai tesque), and MM agar (MM containing 1.5% [wt/vol] Bacto Agar). The growth media were supplemented with regents and antibiotics when necessary at the following concentrations: L(+)-arabinose (Wako), 1 mM; IPTG (Wako), 0.3 mM or 30 mM; X-gal (TaKaRa), 40 μg/mL; sucrose (nacalai tesque), 10% (wt/vol); D-(+)-glucose, 0.1%, 0.2%, or 0.4% (wt/vol); lactose monohydrate, 1.0% (wt/vol); D(+)-maltose monohydrate (Wako), 1.0% (wt/vol); chloramphenicol (Wako), 20 μg/mL; ampicillin (Sigma), 50 μg/mL; tetracycline (nacalai tesque), 10 μg/mL.