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C57bl 6 tg cetp uctp20pnu j

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C57BL/6-Tg(CETP)UCTP20Pnu/J is a transgenic mouse strain that expresses the human cholesteryl ester transfer protein (CETP) gene. This mouse model is used in research related to lipid metabolism and cardiovascular disease.

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Shadv 252885, by Vector Biolabs (2 mentions) Triton wr 1339, by Merck Group (1 mentions)

3 protocols using c57bl 6 tg cetp uctp20pnu j

1

Triglyceride Metabolism Modulation

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TG production was measured in 3-h fasted mice after intravenous administration of Triton WR-1339 (500 mg/kg, Sigma, Saint Louis, MO, USA), an inhibitor of lipolysis. Postprandial TG clearance was measured after 10 weeks of ad libitum access to 20% fructose supplementation to drinking water or with transgenic expression of Cholesteryl Ester Transfer Protein (CETP), a genetic model of impaired TG clearance [44 (link)]. Transgenic CETP mice (C57BL/6-Tg(CETP)UCTP20Pnu/J, Strain: 001929, Jackson Laboratories), were bred with Δhep mice to generate fl/fl (CETP+/SHPfl/fl) and Δhep (CETP+/SHPΔhep) mice. TG clearance was measured in 12-h fasted mice after oral gavage of olive oil (200 μL/mouse) with serial tail blood samples over 7–8 h.
Palmisano B.T., Zhu L., Litts B., Burman A., Yu S., Neuman J.C., Anozie U., Luu T.N., Edington E.M, & Stafford J.M. (2021). Hepatocyte Small Heterodimer Partner Mediates Sex-Specific Effects on Triglyceride Metabolism via Androgen Receptor in Male Mice. Metabolites, 11(5), 330.
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2

Modulating Androgen Receptor in Mice

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All mouse experiments were approved under the Vanderbilt University Institutional Animal Care and Use Committee. Mice were housed in 12hr light/dark cycles in temperature and humidity-controlled facilities with ad-libitum access to chow diet and water. Transgenic CETP were purchased from the Jackson Laboratories (C57BL/6-Tg(CETP)UCTP20Pnu/J, Stock No: 001929). Non-transgenic littermates were used as WT controls. Male mice used in this study were aged 3–4 months old to ensure complete sexual development. All animals were sacrificed between 8 and 11 am to minimize circadian variation in gene expression. Adeno-associated virus serotype 8 (AAV8) containing short-hairpin RNA (shRNA) specific to mouse androgen receptor was purchased from Vector Biolabs (shADV-252885) with the shRNA under control of a U6 promoter and a GFP reporter under control of a CMV promoter. AAV8 has been shown to have tropism for liver (Tenney et al., 2014 (link)). 1.8E12 GC/mouse was injected intravenously under brief isoflurane anesthesia (5%). Euthanasia was achieved using carbon dioxide and confirmed with cervical dislocation.
Palmisano B.T., Anozie U., Yu S., Neuman J.C., Zhu L., Edington E.M., Luu T, & Stafford J.M. (2020). Cholesteryl Ester Transfer Protein Impairs Triglyceride Clearance via Androgen Receptor in Male Mice. Lipids, 56(1), 17-29.
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3

Androgen Receptor Knockdown in Transgenic CETP Mice

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All mouse experiments were approved under the Vanderbilt University Institutional Animal Care and Use Committee. Mice were housed in 12 h light/dark cycles in temperature and humidity‐controlled facilities with ad libitum access to chow diet and water. Transgenic CETP were purchased from the Jackson Laboratories (C57BL/6‐Tg(CETP)UCTP20Pnu/J, Stock No: 001929). Non‐transgenic littermates were used as WT controls. Male mice used in this study were aged 3–4 months old to ensure complete sexual development. All animals were sacrificed between 8 and 11 am to minimize circadian variation in gene expression. Adeno‐associated virus serotype 8 (AAV8) containing short‐hairpin RNA (shRNA) specific to mouse androgen receptor (AR) was purchased from Vector Biolabs (shADV‐252,885, Malvern, PA) with the shRNA under control of a U6 promoter and a green fluorescence protein (GFP) reporter under control of a cytomegalovirus (CMV) promoter. AAV8 has been shown to have tropism for liver (Tenney et al., 2014). 1.8E12 GC/mouse was injected intravenously under brief isoflurane anesthesia (5%). Euthanasia was achieved using carbon dioxide and confirmed with cervical dislocation.
Palmisano B.T., Anozie U., Yu S., Neuman J.C., Zhu L., Edington E.M., Luu T, & Stafford J.M. (2020). Cholesteryl Ester Transfer Protein Impairs Triglyceride Clearance via Androgen Receptor in Male Mice. Lipids, 56(1), 17-29.
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