All chemicals were of analytical grade and used with no further treatment. Graphite flakes (Alfa Aesar, 99.9%, -325 mesh), sodium nitrate (Fluka Chemika, 99%, NaNO3), potassium permanganate (Ajax FineChem, 99.0%, KMnO4), sodium hydroxide (Analytical reagent, Ajax FineChem, NaOH), and copper(ii ) nitrate (Sigma Aldrich, Cu(NO3)2·3H2O) were purchased and used as received. Sulfuric acid (RCI Labscan, 98%, concentrated H2SO4), hydrochloric acid (RCI Labscan, 37%, HCl), hydrogen peroxide (Merck, 30%, H2O2), ethanol (RCI Labscan, 99.9%, C2H5OH), titanium(iv ) butoxide (reagent grade, Sigma Aldrich, C16H36O4Ti) were used as received. The CO2 gas (99.9% purity) was purchased from Lor Ching Tong Oxygen (Thailand).
Labscan
Manufactured by RCI Labscan
Sourced in Thailand
Labscan is a compact and versatile laboratory instrument designed for analyzing various samples. It utilizes advanced spectroscopy techniques to provide accurate data and measurements. The core function of Labscan is to perform quick and reliable analysis across a range of applications.
Automatically generated - may contain errors
Lab products found in correlation
Dpx mountant, by Merck Group (2 mentions)
Hm 525 cryostat, by Thermo Fisher Scientific (2 mentions)
Cresyl violet, by Merck Group (2 mentions)
Light microscope model bh 2, by Olympus (2 mentions)
Paraformaldehyde (pfa), by Merck Group (2 mentions)
Methanol, by Merck Group (2 mentions)
Methanol, by RCI Labscan (2 mentions)
Potassium permanganate, by Thermo Fisher Scientific (1 mentions)
Copper 2 nitrate, by Merck Group (1 mentions)
C16h36o4ti, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Thermo Fisher Scientific (1 mentions)
Graphite flake, by Thermo Fisher Scientific (1 mentions)
Aluminum potassium sulfate, by Merck Group (1 mentions)
Sucrose, by Merck Group (1 mentions)
Sodium silicate, by Merck Group (1 mentions)
Levulinic acid, by Merck Group (1 mentions)
Perchloric acid, by ITW Reagents (1 mentions)
Ammonium persulfate ap, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Coomassie brilliant blue r 250 solution, by Bio-Rad (1 mentions)
25 protocols using labscan
1
Synthesis of Graphene Oxide Nanosheets
2
Quantitative Analysis of Fe-TA NPs in Liver
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For quantitative accumulation of Fe–TA NPs analysis, quantity of liver was homogenized in a mixture of pure nitric (≥69%) (RCI Labscan) and hydrochloric acid (≥37%) (RCI Labscan) for 24 hours at room temperature. The homogenate was centrifuged at 10000g for 10 minutes. The supernatant was collected and was further diluted in 2% mixed acid solution. The solution was subjected to ICP-OES analysis for iron content determination according to standard protocol.
3
Standardized Herbal Capsule Production
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Glucose powder was purchased from Utopian Co. Ltd., (Samutprakan), Thailand, and 17-α-hydroxyprogesterone was purchased from Sigma, (Shanghai) China. Acetonitrile was analytical grade from RCI Labscan, (Bangkok) Thailand. Methanol was HPLC grade from RCI Labscan, (Bangkok), Thailand.
Dried powder of KP Romkaou strain rhizomes obtained from Phurue, Loei province of Thailand was authenticated and kept as a voucher specimen (No. KP-BS-2010) at the Center for Research and Development of Herbal Health Products, Khon Kaen University, (Khon Kaen), Thailand. KP extract was prepared by maceration in 95% ethanol (following the petty patent of Thailand No. 4048) and the crude extract was obtained at 5.71% yield. The KP extract was analyzed for the content of methoxyflavones using HPLC (Figure 1 ). The tested KP product was prepared in the dosage form of a capsule to contain 90 mg of KP extract and other excipients (Table 1 ). The placebo capsule containing no KP extract was composed of the same excipients and capsule color. The entire study was conducted using a single batch of KP extract to optimize product consistency.
Dried powder of KP Romkaou strain rhizomes obtained from Phurue, Loei province of Thailand was authenticated and kept as a voucher specimen (No. KP-BS-2010) at the Center for Research and Development of Herbal Health Products, Khon Kaen University, (Khon Kaen), Thailand. KP extract was prepared by maceration in 95% ethanol (following the petty patent of Thailand No. 4048) and the crude extract was obtained at 5.71% yield. The KP extract was analyzed for the content of methoxyflavones using HPLC (
4
Synthesis of SEPS and SEES Copolymers
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SIS and SBS copolymers were used as received. The SIS triblock copolymer was obtained from Kraton Polymers with a total molecular weight of 58,200 g mol−1 (MW,PS = 17,460 g mol−1 and MW,PI = 40,740 g mol−1) and a weight fraction, Φps = 0.3. The SBS triblock copolymer was kindly supplied by Repsol-YPF (Dynasol C540, Madrid, Spain) with a total molecular weight of 75,000 g mol−1 (MW,PS = 30,000 g mol−1 and MW,PI = 45,000 g mol−1) and a weight fraction, Φps = 0.4. The hydrogenation procedure for obtaining SEPS and SEES was conducted with p-toluenesulfonyl hydrazide (Sigma-Aldrich (Saint Louis, MO, USA), 97%) and it is described elsewhere [60 (link)]. Toluene (Lab Scan, HPLC, 99.8%, Samut Sakhon, Thailand), cyclohexane (Panreac, 99.5%, Barcelona, Spain), and tetrahydrofuran (THF; Lab-scan, 99.8%, Samut Sakhon, Thailand) were used as solvents without any further purification.
5
Cresyl Violet Staining of Hippocampal Neurons
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The brains were perfused transcardially with fixative solution containing 4% paraformaldehyde (Sigma-Aldrich, USA) in 0.1 M phosphate buffer pH 7.4 overnight at 4°C. Then, they were infiltrated with 30% sucrose (Merck, Germany) solution for 48–72 h. Serial sections of tissues were cut frozen on cryostat (Thermo Scientific™ HM 525 Cryostat) at 10 μm thick. All sections were picked up on slides coated with 0.3% aqueous solution of gelatin containing 0.05% aluminum potassium sulfate (Sigma-Aldrich, USA). The triplicate coronal sections of the brains were stained with 0.25% cresyl violet (Sigma-Aldrich, USA), dehydrated through graded alcohols (70, 95, 100% 2x) (RCI LabScan, Thailand), placed in xylene (Merck, Germany), and mounted using DPX mountant (Merck, Germany). The evaluation of neuron density in the hippocampus was performed under Olympus light microscope model BH-2 (Japan) at 40x magnification. Counts were performed in three adjacent fields, and the mean number was calculated and expressed as the density of neurons per 255 μm2.
6
Synthesis of Hierarchical ZSM-12 Nanolayers
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Sodium silicate (Na2Si3O7: 26.5 wt% SiO2, and 10.6 wt% Na2O, Merck), aluminium sulphate (Al2(SO4)3·18H2O, Univar, Ajax Finechem), sulfuric acid (H2SO4: 96%, RCI Labscan), sodium hydroxide (NaOH: 98%, Carlo Erba), α,α′-dichloro-p-xylene (98%, TCI), N,N,N′,N′-tetramethyl-1,6-hexanediamine (98%, TCI), and dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride (TPOAC: 42 wt% in methanol, Aldrich) were used as starting materials for the synthesis of hierarchical ZSM-12 nanolayers. Levulinic acid (>97%, TCI), toluene (≥99.9%, Merck), ethanol (99.9%, QRec), and decane (≥99.9%, TCI) were used for the catalytic activity testing without any further purification.
7
Synthesis and Characterization of PNMA
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NMA monomer (AR, 99.5%) was purchased from TCI (Tokyo Chemical Industry, Tokyo, Japan). Ammonium persulfate (APS; AR, 99.5%) was purchased from Merck (Merck, Darmstadt, Germany). SDS (AR, >99%) was purchased from OmiPur (Merck, Darmstadt, Germany). SDBS (AR, >99%) and AOT (AR, >99%) were purchased from Sigma Aldrich (Sigma-Aldrich, Dorset Gillingham, UK). The chemical structures of surfactants used in this work are shown in Supplementary (Table S1) . Ethanol (AR, 99%) for dissolving NMA monomer was from Merck (Merck, Darmstadt, Germany). Hydrochloric acid (HCl; AR, 37% v/v) was from RCI Labscan (RCI Labscan, Bangkok, Thailand). Ammonium hydroxide (NH4OH; Panreac 25% v/v) was employed to de-dope the synthesized PNMA powder whereas perchloric acid (HClO4; Panreac, 70% v/v,) was used as a doping agent. Dimethyl sulfoxide (DMSO; Merck, AR, >99%) used to prepare the PNMA solution for the UV-vis spectroscopy. Distilled water was used as a solvent. All reagents were used without further modification.
8
Electrochemical Cell Fabrication
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Acetone (RCI Labscan, 99,5%), ethanol (Sigma Aldrich, 97%), nitric acid (Sigma Aldrich, 70%), hydrochloric acid (Sigma Aldrich, 37%), deionized water, and DuPont Krytox GPL 103 were used without further purification. The dimensions of the purchased indium tin oxide (ITO) glass slides are 2.5 cm × 7.5 cm × 2.5 mm. The thickness and average pore size of the porous PTFE membrane (Sterlitech Corporation, PTU023001) are 25–50 μm and 200 nm, respectively.
9
Fibroin Protein Separation by SDS-PAGE
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Protein in the isolated fibroin was separated using the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) method on a 5% stacking gel and a 12% separating gel. After electrophoresis, the gel was stained in Coomassie Brilliant Blue R-250 solution (BIO-RAD Laboratories) for 2 h and de-stained with a mixture of 7% acetic acid (VWR International Ltd., Poole, UK) and 10% methanol (RCI Labscan) in water. The molecular weights of protein bands were estimated by comparing them with standard molecular weight markers.
10
Quantitative Analysis of Herbal Extracts
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Ammonium acetate was (purity ≥ 98%, chromatographic grade) purchased from Sigma-Aldrich (St. Louis, MO, USA). Deionized water was prepared by Millipore Milli Q-Plus system (Bedford, MA, USA). 98% sulfuric acid from RCI Labscan (Bangkok, Thailand), ethanol (analytical grade) from Merck (Darmstadt, Germany), and phenol from Sigma-Aldrich were used. The dextran and pullulan reference substances (Figure 6 ) with known molecular sizes (1–670 kDa for dextrans, 6–805 kDa for pullulans), and d -glucose were purchased from Sigma-Aldrich and Shodex (Tokyo, Japan). The herbal materials of GR, AR and DO were purchased from their geo-authentic product areas, i.e., the Jilin, Inner Mongilia and Anhui Province of PRC, respectively, and authenticated by Prof. Hu-Biao Chen. Voucher specimens of these samples were deposited at the School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong.
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