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2 protocols using potato dextrose broth (pdb)

1

Exopolysaccharide Production by Lactobacillus plantarum

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Lactobacillus plantarum LLC-605 was obtained from the traditional Chinese fermented food FuYuan Pickles (Yunnan, China) with excellent exopolysaccharide-producing ability in our lab (Li et al., 2017 (link)). E. coli, S. aureus, M. luteus, B. subtilis, and P. pastoris were ordered from China Center of Industrial Culture Collection (CICC, Beijing, China). Extracellular polysaccharide EPS-605 was purified from the fermentation broth of L. plantarum LCC-605, as described in our previous study (Li et al., 2017 (link)). Dialysis membranes (Spectra/Por6 Dialysis membranes, Regenerated Cellulose) with a molecular weight cutoff of 1 kDa were purchased from Sangon Biotech. Co., Ltd. (Shanghai, China). Lysogeny broth (LB), potato dextrose agar (PDA), and potato dextrose broth (PDB) were ordered from Beijing Land Bridge Technology (Beijing, China).
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2

Mycotoxin Detection in Food Samples

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Nutrient agar (NA) and nutrient broth (NB) were purchased from Qingdao Hope Bio-Technology Co., Ltd. (Qingdao, China). Potato dextrose agar (PDA) and potato dextrose broth (PDB) were provided by Beijing Land Bridge Technology Co., Ltd. (Beijing, China). LC-MS grade acetonitrile and acetic acid were supplied by Thermo Fisher Scientific (Waltham, MA, USA). Standard solutions of TeA, AOH, AME, and TEN were purchased from Romar Labs Division Holding GmbH (Getzersdorf, Austria). Propidium iodide (PI) solution was provided by Sigma-Aldrich, Co., Ltd. (Burlington, MA, USA). The Fungal RNA Kit was purchased from Omega Bio-tek, Inc. (Norcross, GA, USA). The FastKing Rt Kit (With gDNase) and SuperReal PreMix Plus (SYBR Green) were purchased from Tiangen Biotech Co., Ltd. (Beijing, China).
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