Anti gclc antibody ab55435

Manufactured by Abcam

Sourced in China

Anti-GCLC antibody (ab55435) is a laboratory reagent used to detect the presence and quantify the expression levels of the GCLC protein. GCLC, also known as glutamate-cysteine ligase catalytic subunit, is a key enzyme involved in the synthesis of glutathione, an important antioxidant. This antibody can be used in various immunoassay techniques to study the role of GCLC in cellular processes.

Automatically generated - may contain errors

Lab products found in correlation

Cell counting kit 8 (cck8), by Dojindo Laboratories (2 mentions) Ab102032, by Abcam (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Sc 130335, by Santa Cruz Biotechnology (1 mentions) Rna assay reagents, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Anti-GCLC (8 citations) Ab55435 (3 citations)

2 protocols using anti gclc antibody ab55435

Investigating iKeap1 Modulation of Oxidative Stress

Cited 1 time

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iKeap1 was synthesized by Ruilu Chemicals (Shanghai, China) based on its structure [24 (link)]. Dexamethasone (Dex), nicotine, and H₂O₂ were provided by Sigma Aldrich Chemicals (St Louis, Mo). Antibodies for HO1 (#70081), NQO1 (#3187), Nrf2 (#12721), Keap1 (#8047), Tubulin (#2125), and Lamin B1 (#13435) as well as cleaved-caspase-3 (#9664), cleaved-poly (ADP-ribose) polymerase (PARP, #5625) and cleaved-caspase-9 (#20750) were provided by Cell Signaling Tech China (Shanghai, China). The anti-GCLC antibody (ab55435) and the anti-adenine nucleotide translocase-1 (ANT1) antibody (ab102032) were purchased from Abcam China (Shanghai, China). The anti-cyclophilin-D (CyPD, sc-137136) antibody was purchased from Santa Cruz Biotech (Santa Cruz, CA). From Thermo Fisher Invitrogen (Suzhou, China) RNA assay reagents were obtained. Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Laboratories (Kumamoto, Japan). All cell culture reagents, including fetal bovine serum, DMEM, and antibiotics, were provided by Gibco (Shanghai, China).

Zheng Y.H., Yang J.J., Tang P.J., Zhu Y., Chen Z., She C., Chen G., Cao P, & Xu X.Y. (2021). A novel Keap1 inhibitor iKeap1 activates Nrf2 signaling and ameliorates hydrogen peroxide-induced oxidative injury and apoptosis in osteoblasts. Cell Death & Disease, 12(7), 679.

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Synthesis and Analysis of 4-OI

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Four-octyl itaconate (4-OI) was synthesized by Ruilu Chemicals (Shanghai, China). H₂O₂ was provided by Sigma Aldrich Chemicals (St Louis, Mo). Antibodies for HO1 (#70081), NQO1 (#3187), Nrf2 (#12721), Keap1 (#8047), Tubulin (#2125), and Lamin B1 (#13435), as well as cleaved-poly (ADP-ribose) polymerase (PARP, #5625), cleaved-caspase-9 (#20750), and immunoresponsive gene 1 (IRG1, #77510) were obtained from Cell Signaling Tech China (Shanghai, China). The anti-GCLC antibody (ab55435) and the anti-adenine nucleotide translocase-1 (ANT1) antibody (ab102032) were purchased from Abcam China (Shanghai, China). Antibodies of PGK1 (sc-130335) and cyclophilin D (CyPD, sc-137136) were provided by Santa Cruz Biotech (Santa Cruz, CA). RNA assay reagents were obtained from Thermo-Fisher Invitrogen (Suzhou, China). Cell Counting Kit-8 (CCK-8) was provided by Dojindo Laboratories (Kumamoto, Japan).

Zheng Y., Chen Z., She C., Lin Y., Hong Y., Shi L., Zhang Y., Cao P, & Xu X. (2020). Four-octyl itaconate activates Nrf2 cascade to protect osteoblasts from hydrogen peroxide-induced oxidative injury. Cell Death & Disease, 11(9), 772.

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