Ecl supersignal detection system

Manufactured by Thermo Fisher Scientific

Sourced in Sweden

The ECL SuperSignal detection system is a chemiluminescent substrate used for the detection of proteins in Western blot analysis. It generates a light signal when exposed to the enzyme horseradish peroxidase, allowing for the visualization and quantification of target proteins.

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Lab products found in correlation

Anti myc, by Santa Cruz Biotechnology (1 mentions) Anti ha, by Santa Cruz Biotechnology (1 mentions) Anti flag, by Merck Group (1 mentions) Anti tubulin antibody, by Merck Group (1 mentions) Anti ha antibody, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

SuperSignal West Pico ECL detection system ECL SuperSignal West Pico Chemiluminescent Substrate Detection System SuperSignal ECL system SuperSignal detection system ECL detection system SuperSignal ECL SuperSignal system ECL system

4 protocols using ecl supersignal detection system

Western Blotting of Ribonucleotide Reductase

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Protein samples for Western blotting were prepared as described before [59 ]. Proteins were resolved by SDS-PAGE 10–15% acrylamide gels and transferred to nitrocellulose membranes, blocked with 5% milk in TBST and immunoblotted with the indicated antibodies. Detection was carried out using the ECL SuperSignal detection system (Thermo Scientific).
Rabbit polyclonal anti-Rnr1 (AS09 576), anti-Rnr3 (AS09 574), and anti-Sml1 (AS10 847) antibodies were produced by Agrisera, Sweden. For the detection of both Rnr4 and α-tubulin we used YL1/2 rat monoclonal antibodies (Sigma).

Maicher A., Gazy I., Sharma S., Marjavaara L., Grinberg G., Shemesh K., Chabes A, & Kupiec M. (2017). Rnr1, but not Rnr3, facilitates the sustained telomerase-dependent elongation of telomeres. PLoS Genetics, 13(10), e1007082.

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Protein Detection by Western Blotting

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Proteins were resolved by SDS-PAGE 10–15% acrylamide gels and transferred to nitrocellulose membranes, blocked with 5% milk in TBST and immunoblotted with anti-HA (Santa cruz, SC7392), anti-Actin (MBP, #08691001), anti-MYC (Santa cruz, SC40) or anti-FLAG (Sigma, F1804) as indicated. Detection was carried out using the ECL SuperSignal detection system (Thermo Scientific).

Cohen A., Pataki E., Kupiec M, & Weisman R. (2022). TOR complex 2 contributes to regulation of gene expression via inhibiting Gcn5 recruitment to subtelomeric and DNA replication stress genes. PLoS Genetics, 18(2), e1010061.

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Immunoblotting for Gaf1-HA and Tubulin

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Proteins were resolved by SDS-PAGE using 12% acrylamide gels. Proteins were transferred to nitrocellulose membranes and blocked with 5% milk in TBST before immunoblotting. Detection was carried out using the ECL Super Signal detection system (Thermo Scientific). Gaf1-HA was detected by anti-HA antibodies (Santa Cruz) and tubulin with anti-tubulin antibody (Sigma).

Laor D., Cohen A., Kupiec M, & Weisman R. (2015). TORC1 Regulates Developmental Responses to Nitrogen Stress via Regulation of the GATA Transcription Factor Gaf1. mBio, 6(4), e00959-15.

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Phosphorylation Detection of Gad8 and Chk1

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Proteins were resolved by SDS-PAGE 10–15% acrylamide gels and transferred to nitrocellulose membranes, blocked with 5% milk in TBST and immunoblotted with the indicated antibodies. Detection was carried out using the ECL SuperSignal detection system (Thermo Scientific). Gad8 Thr387 phosphorylation events were detected using total protein extracts by phosphospecific antibodies. Antibodies against Gad8 Thr387-P were raised against the Gad8 phosphopeptide CRFANWpSYQRPT [59 (link)]. To follow Chk1 phosphorylation, proteins were extracted with trichloroacetic acid (TCA) as described previously and resolved by SDS-PAGE using 8% acrylamide gels.

Pataki E., Simhaev L., Engel H., Cohen A., Kupiec M, & Weisman R. (2020). TOR Complex 2- independent mutations in the regulatory PIF pocket of Gad8AKT1/SGK1 define separate branches of the stress response mechanisms in fission yeast. PLoS Genetics, 16(11), e1009196.

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