3β adiol

Manufactured by Steraloids

Sourced in United States

3β-adiol is a steroid compound that functions as a metabolite of dihydrotestosterone (DHT). It is commonly used in research applications involving the study of androgen receptor signaling and related biological processes.

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Lab products found in correlation

3α adiol, by Steraloids (2 mentions) Sulfatase from abalone entrails, by Merck Group (1 mentions) High performance liquid chromatography (hplc), by Thermo Fisher Scientific (1 mentions) Testosterone, by Steraloids (1 mentions) β glucuronidase from e coli, by Merck Group (1 mentions) Charcoal dextran stripped fetal bovine serum cd fbs, by Atlanta Biologicals (1 mentions) Optima lc ms, by Thermo Fisher Scientific (1 mentions) Estradiol valerate, by Merck Group (1 mentions) Genistein, by Merck Group (1 mentions) 17β estradiol, by Merck Group (1 mentions) 4 dimethylaminopyridine dmap, by Tokyo Chemical Industry (1 mentions) Oasis max cartridge, by Waters Corporation (1 mentions) 7α oh dhea, by Steraloids (1 mentions) R1881, by Merck Group (1 mentions) Testosterone, by Merck Group (1 mentions)

Spelling variants of this product

Adiol (1 citations)

4 protocols using 3β adiol

Androgen Effects on Tumor Cell Proliferation

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Tumour cell proliferation was evaluated by BrdU-ELISA. Cells were treated with 0.1, 1, or 10 nmol/L testosterone (article number: T6147, Sigma-Aldrich, Taufkirchen, Germany); 0.1, 1, 5, or 10 nmol/L DHT (article number: D5027, Sigma-Aldrich, Taufkirchen, Germany) and R1881 (article number: R0908, Sigma-Aldrich, Taufkirchen, Germany); and 0.01, 0.1, 1, 5, 10, 100, 250, or 1000 nmol/L 3α-adiol and 3β-adiol (article number: A1170-000 for 3α-adiol and A1220-000 for 3β-adiol, both from Steraloids, Newport, Rhode Island, USA) for 48 h.

Bremmer F., Jarry H., Unterkircher V., Kaulfuss S., Burfeind P., Radzun H.J., Ströbel P, & Thelen P. (2018). Testosterone metabolites inhibit proliferation of castration- and therapy-resistant prostate cancer. Oncotarget, 9(24), 16951-16961.

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Steroid hormone quantification protocol

Cited 1 time

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Reagents were of ACS grade or higher (e.g. HPLC and Optima LC/MS) and were purchased from Thermo Fisher Scientific and used without further purification. Testosterone, epiTestosterone (epi-T), DHEA, DHEA-S sodium salt, DHEA glucuronide (DHEA-G), AST, epi-AST, DHT, 5-adiol, 3α-adiol and 3β-adiol were purchased from Steraloids (Wilton, NH, USA). [2,3,4-¹³C₃]-T ([¹³C₃]-T) and [2,3,4-¹³C₃]-DHT ([¹³C₃]-DHT) were from C/D/N Isotopes (Point-Claire, Quebec, Canada) and Cambridge Isotopes (Andover, MA, USA), respectively. [2,3,4-¹³C₃]-3α-adiol ([¹³C₃]-3α-adiol) and [2,3,4-¹³C₃]-3β-adiol ([¹³C₃]-3β-adiol) were synthesized by an enzymatic method according to our published procedure (Zang et al. 2017 (link)). 4-Dimethylaminopyridine (DAP) 2-methyl-6-nitrobenzoic anhydride (MNBAn), picolinic acid (PA), triethylamine (TEA), anhydrous tetrahydrofuran (THF), β-glucuronidase from E. coli and sulfatase from Abalone entrails were from Sigma-Aldrich. Charcoal dextran stripped fetal bovine serum (CD-FBS) was from Atlanta Biologicals (Lawrenceville, GA, USA).

Zang T., Taplin M.E., Tamae D., Xie W., Mesaros C., Zhang Z., Bubley G., Montgomery B., Balk S.P., Mostaghel E.A., Blair I.A, & Penning T.M. (2017). Testicular vs adrenal sources of hydroxy-androgens in prostate cancer. Endocrine-related cancer, 24(8), 393-404.

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Estrogen and Selective Modulators

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17β-estradiol, estradiol valerate and genistein were from Sigma-Aldrich (St. Louis, MO, USA). 3β-Adiol was from Steraloids (London, UK); raloxifen and tamoxifen were kindly obtained from Siena Biotech (Siena, Italy).

Piccolella M., Crippa V., Cristofani R., Rusmini P., Galbiati M., Cicardi M.E., Meroni M., Ferri N., Morelli F.F., Carra S., Messi E, & Poletti A. (2017). The small heat shock protein B8 (HSPB8) modulates proliferation and migration of breast cancer cells. Oncotarget, 8(6), 10400-10415.

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Steroid Metabolite Quantification Protocol

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F, DHEA, Δ5-diol, E2, and fusaric acid (FA) were from Sigma-Aldrich (St. Louis, MO, USA). 7α-OH-DHEA and 3βAdiol were from Steraloids (Newport, RI, USA). F-d₄ was from C/D/N Isotopes Inc. (Quebec, Canada). DHEA-¹³C₃ was synthesized at Tohoku Pharmaceutical University (Miyagi, Japan). Δ5-diol-d₄ and 7α-OH-DHEA-d₂ were synthesized at ASKA Pharma Medical Co. Ltd. (Kanagawa, Japan). E2-¹³C₄ was from Hayashi Pure Chemical Ind. Ltd. (Osaka, Japan). 4-dimethylaminopyridine (DMAP), 2-methyl-6-nitrobenzoic anhydride (MNBA), and picolinic acid (PA) were from Tokyo Chemical Industry (Tokyo, Japan). Triethylamine (TEA), O-ethylhydroxyl-ammonium chloride, pentafluoropyridine, and 1 M sodium hydroxide solutions were from Wako Pure Chemical Industries (Osaka, Japan). OASIS MAX cartridge (60 mg, 3 mL) was from Waters Corporation (Milford, MA, USA). InertSep SI cartridge (500 mg, 3 mL) was from GL Science (Tokyo, Japan). All other reagents and solvents were of analytical grade.

Kobayashi M., Sugiyama N., Sasayama D., Sasamoto H., Miyashiro Y., Arima K, & Washizuka S. (2016). Sex differences in the serum level of endogenous ligands for estrogen receptor β in the elderly population. Scientific Reports, 6, 25878.

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