Total and oligomeric CSF α-syn were measured as previously reported (Tokuda et al., 2006 (link)). Briefly, for CSF t-α-syn anti-human α-syn monoclonal antibody (clone Syn211) (Santa Cruz Biotechnology, USA) was used for capturing while the anti-human α-syn polyclonal antibody FL-140 (Santa Cruz Biotechnology, USA) was used for antigen detection. The standard curve for the ELISA assay was constructed using recombinant human α-syn solution at different concentrations diluted in blocking buffer. For α-syn oligomers, the antibody clone Syn211 was used for capturing, while biotinylated Syn211 (Santa Cruz Biotechnology, USA) was used for antigen detection. The plate was incubated with 50 μ L/well of ExtrAvidin-Peroxidase (Sigma-Aldrich, UK) and with the enhanced chemiluminescent substrate. For both immunoassays, the samples were screened in blind fashion and were randomly tested. A series of internal controls were run to check for run-to-run variations.
Biotinylated syn211
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Biotinylated Syn211 is a chemical compound that contains a biotin molecule covalently attached to the Syn211 protein. Syn211 is a protein that is commonly used in research applications. The biotin molecule allows for the detection and labeling of Syn211 in various experimental techniques.
Automatically generated - may contain errors
2 protocols using biotinylated syn211
1
ELISA Quantification of Total and Oligomeric α-Synuclein in CSF
2
Quantification of CSF α-Synuclein Isoforms
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Total and oligomeric CSF α-syn were measured as reported.15 (link),20 (link) Briefly, for CSF total α-syn, an anti-human α-syn monoclonal antibody (clone Syn211) (Santa Cruz Biotechnology, Santa Cruz, CA, USA) was used as primary antibody to capture a-syn species, while the anti-human α-syn polyclonal antibody FL-140 (Santa Cruz Biotechnology, Santa Cruz, CA, USA) was used for antigen detection. The standard curve for the enzyme-linked immunosorbent assay (ELISA) was constructed using recombinant human α-syn solution at different concentrations diluted in blocking buffer.
For α-syn oligomers, the antibody clone Syn211 was used for capturing, while biotinylated Syn211 (Santa Cruz Biotechnology, Santa Cruz, CA, USA) was used for antigen detection. The plate was incubated with 50 μL/well of ExtrAvidin-Peroxidase (Sigma-Aldrich, Dorset, UK) and with the enhanced chemiluminescent substrate. For both immunoassays, the samples were screened in blind fashion and were randomly tested. A series of internal controls were run to check for run-to-run variations.
Total tau and phosphorylated tau 181 were measured with commercially available ELISA kits (Innotest hTAU-Ag, p-TAU 181 Ag; Innogenetics NV, Ghent, Belgium).
For α-syn oligomers, the antibody clone Syn211 was used for capturing, while biotinylated Syn211 (Santa Cruz Biotechnology, Santa Cruz, CA, USA) was used for antigen detection. The plate was incubated with 50 μL/well of ExtrAvidin-Peroxidase (Sigma-Aldrich, Dorset, UK) and with the enhanced chemiluminescent substrate. For both immunoassays, the samples were screened in blind fashion and were randomly tested. A series of internal controls were run to check for run-to-run variations.
Total tau and phosphorylated tau 181 were measured with commercially available ELISA kits (Innotest hTAU-Ag, p-TAU 181 Ag; Innogenetics NV, Ghent, Belgium).
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