Monoclonal mouse anti-FAK (4.47, 05-0537) and anti-phospho Src (Tyr 416, 05-677) antibodies were obtained from Millipore (EMD Millipore, Billerica, MA) and rabbit polyclonal anti-phospho-FAK (Y397, 71-7900) and anti-phospho Erk 1/2 (05-797R) antibodies were from Invitrogen (Invitrogen Corp., Carlsbad, CA). Rabbit polyclonal anti-PARP (9542S), anti-Akt (9272), anti-phospho Akt (Ser 473, 9271), anti-Erk 1/2 (9102) and anti-cleaved caspase 3 (9662) antibodies were obtained from Cell Signaling Technology (Danvers, MA). Monoclonal mouse anti-Jnk (F-3, sc-1648), anti-phospho Jnk (G-7, sc-6254) and rabbit polyclonal anti-c-Src (sc-18) were from Santa Cruz Biotechnology (Santa Cruz, CA). Monoclonal mouse anti-GAPDH was from Millipore (MAB374) and anti-β-actin was from Sigma (A1978, Sigma-Aldrich Corp., St. Louis, MO). The small molecule PF-573,228 (C22H20F3N5O3S) was obtained from Pfizer (New York, NY), and the small molecule 1,2,4,5-benzenetetraamine tetrahydrochloride (Y15) (C6H10N4·4ClH) from Sigma.
Anti c src sc 18
Manufactured by Santa Cruz Biotechnology
Anti-c-Src (sc-18) is a primary antibody that specifically recognizes the c-Src protein. c-Src is a non-receptor tyrosine kinase that plays a role in various cellular processes. This antibody can be used for the detection of c-Src in a variety of applications, such as Western blotting, immunoprecipitation, and immunohistochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Anti cleaved caspase 3 9662, by Cell Signaling Technology (1 mentions)
Pf 573 228 c22h20f3n5o3s, by Pfizer (1 mentions)
1 2 4 5 benzenetetraamine tetrahydrochloride y15 c6h10n4 4clh, by Merck Group (1 mentions)
Anti gapdh, by Merck Group (1 mentions)
Anti β actin, by Merck Group (1 mentions)
Anti flag f7425, by Merck Group (1 mentions)
Anti ha antibody 3f10, by Roche (1 mentions)
Sheep anti mouse igg na931, by GE Healthcare (1 mentions)
Hrp conjugated donkey anti goat igg sc 2020, by Santa Cruz Biotechnology (1 mentions)
Protein a g sepharose, by Merck Group (1 mentions)
Anti egfr sc 03, by Santa Cruz Biotechnology (1 mentions)
3 protocols using anti c src sc 18
1
Immunoblotting Antibodies for Focal Adhesion Kinase
2
Antibody Characterization for Cell Signaling
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The anti-MRTF-A/B and anti-caldesmon antibodies were described previously [16 (link), 45 (link)]. Anti-tropomyosin (T2080), anti-α-actin (A5441), anti-vinculin (V9131), anti-Talin (T3287), anti-tubulin (T9024), and anti-FLAG (F7425) antibodies were from Sigma. Anti-SRF (sc-335), anti-zyxin (sc-6437), anti-Myc (sc-789), anti-RhoA (sc-418), anti-MYPT1 (sc-25618) and anti-c-Src (sc-18) antibodies were from Santa Cruz. Anti-pY118-phosphopaxillin antibody (#3818-1) was from Epitomics, anti-paxillin (MA3060) and anti-integrin αv (AB1930) antibodies from Chemicon, anti-GAPDH antibody (M171-3) from MBL, anti-HA antibody (3F10) from Roche, anti-integrin β1 antibody (610467) from BD Transduction Laboratories, and anti-FLAG antibody (018-22386) from Wako. Anti-FAK (#3285), anti-pY397-phosphoFAK (#3283), anti-pY576/577-phosphoFAK (#3281), anti-pY925-phosphoFAK (#3284), anti-pY527-phosphoSrc (#2105), anti-pY416-phosphoSrc family (#6943) and anti-pT696-phosphoMYPT1 (#5163) antibodies were from Cell Signaling Technology. The secondary antibodies, horseradish peroxidase (HRP)-conjugated donkey anti-rabbit IgG (NA934) and sheep anti-mouse IgG (NA931) were from GE Healthcare, HRP-conjugated donkey anti-goat IgG (sc-2020) from Santa Cruz, and Alexa-conjugated goat anti-rabbit IgG (A-11034, A-11036) and goat anti-mouse (A-21049, A-11031) from Thermo Fisher Scientific.
3
GnRH Analog and Signaling Pathway Inhibitors
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[D-Trp6]-GnRH, a stable GnRH analog (GnRH-a), genistein (PTK inhibitor), enolase, and protein A/G-sepharose were obtained from Sigma Chemical (St. Louis, MO, USA). GF109203X, PD098059, SB203580, wortmannin, AG1478, PP1, pertussis-toxin, anti-pan-ras monoclonal antibody, and TPA were purchased from Calbiochem. Polyclonal anti-ERK (C16, sc-093; 1:2500), anti-c-Src (sc-18; 1:1000), anti-phosphotyrosine (PY99, sc7020; 1:1000), anti-phospho EGFR (Tyr 1173, sc-101668; 1:2000), anti-EGFR (sc-03, 1:2000), and anti-Hb-EGF (C-18, sc-1413; 1:1000) antibodies were from Santa Cruz Biotechnology, Inc. Monoclonal anti-diphospho (DP)-ERK (M8159; 1:20,000) and DP-JNK (J4750; 1:2000) antibodies (anti-active ERK and JNK); polyclonal anti-phospho S473 protein kinase B (PKB) antibody (P4112, 1:2000); and polyclonal anti-general ERK (M5670, 1:5000), JNK (J4500; 1:1000), and PKB (P1601; 1:2000) antibodies were from Sigma, Israel (Rehovot, Israel). Polyclonal anti-phospho-(Tyr-416)-Src antibody (anti-active Src; #2101; 1:1000 dilution) was purchased from New England Biolabs.
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