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Tert butylhydroquinone tbhq

Manufactured by Thermo Fisher Scientific
Sourced in United States

Tert-butylhydroquinone (tBHQ) is a synthetic antioxidant used in various food and industrial applications. It functions as a preservative to help maintain the stability and shelf-life of products.

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3 protocols using tert butylhydroquinone tbhq

1

Antioxidant Compound Screening Protocol

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Paraformaldehyde (PFA; Catalog # AAJ19943K2), Phosphate Buffered Saline (PBS; Catalog #AAJ75889AE), Methanol (Catalog #A412-4), Tween-20 (Catalog #BP337-100), and Dimethyl Sulfoxide (DMSO; Catalog #BP231-1) were purchased from Fisher Scientific (Pittsburgh, PA, USA). Tert-Butyl hydroperoxide (tBOOH; Catalog #A13926AP) and N-acetyl-l-cysteine (NAC; Catalog #A1540914) were purchased from Alfa Aesar (Ward Hill, MA, USA). Tert-butyl hydroquinone (tBHQ; Catalog #AC150820050) was purchased from Acros Organics (NJ, USA). dl-Sulforaphane (SFN; Catalog #S4441) was purchased from Millipore-Sigma (Burlington, MA, USA). Vectashield Antifade Mounting Medium with DAPI (Catalog #H-1200) was purchased from Vector Laboratories (Burlingame, CA, USA). Chicken Anti-Rabbit IgG AlexaFluor 594 (Catalog # A-21442), AlexaFluor 594 tagged Streptavidin (Catalog #S32356) and Biotinylated Glutathione Ethyl Ester (BioGEE; Catalog #G36000) were purchased from Invitrogen (Carlsbad, CA, USA).
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2

Preparation of PCB126 and tBHQ Solutions

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Polychlorinated biphenyl-126 (PCB126) was purchased from Ultra Scientific (N. Kingstown, RI, USA), and was dissolved in dimethyl sulfoxide (DMSO, Fisher Scientific, Pittsburgh, PA, USA). Solutions were vortexed prior to use. Tert-butylhydroquinone (tBHQ) was purchased from Acros Organics (Thermo Fisher Scientific, Waltham, MA, USA).
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3

TCDD Exposure and Cellular Response

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2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) was obtained from Ultra Scientific (Hope, RI). Dimethyl sulfoxide (DMSO) was from Sigma-Aldrich (St. Louis, MO). Tert-butylhydroquinone (tBHQ) was obtained from Acros Organics (Geel, Belgium).
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