CCK-8 kit (M4839, ABMOLE, USA) was utilized to perform the experiments. In advance to the experiments, the transfected NSCLC cells were inoculated into 96-well culture plates. Subsequently, the transfected cells were subjected to incubation for 24, 48 and 72 h, followed by CCK-8 solution treatment. Microplate reader (51119770DP, Thermo Fisher) was applied for the calculation of the absorbance value at 450 nm.
Cck 8 kit
Manufactured by AbMole
Sourced in United States, China
The CCK-8 kit is a colorimetric assay for the determination of cell viability in proliferation and cytotoxicity tests. It utilizes the tetrazolium salt WST-8 to produce a water-soluble formazan dye upon reduction by dehydrogenases in viable cells.
Automatically generated - may contain errors
Lab products found in correlation
Gemini em microplate reader, by Molecular Devices (2 mentions)
Microplate reader, by Thermo Fisher Scientific (2 mentions)
Microplate reader, by Dynex (1 mentions)
Annexin 5 fitc pi apoptosis detection kit, by Vazyme (1 mentions)
Prism 5, by GraphPad (1 mentions)
Total rna isolation kit, by Vazyme (1 mentions)
Trypsin edta, by Biosharp (1 mentions)
Hiscribe t7 in vitro transcription kit, by New England Biolabs (1 mentions)
Rnase h, by New England Biolabs (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Opti mem, by Thermo Fisher Scientific (1 mentions)
Chamq universal sybr qpcr master mix, by Vazyme (1 mentions)
Hiscript 3 rt supermix for qpcr, by Vazyme (1 mentions)
Penicillin streptomycin, by Biosharp (1 mentions)
Annexin 5 fitc apoptosis detection kit, by Beyotime (1 mentions)
24 well transwell chamber, by Corning (1 mentions)
Cck 8 solution, by Dojindo Laboratories (1 mentions)
Gemini em, by Molecular Devices (1 mentions)
23 protocols using cck 8 kit
1
CCK-8 Assay for NSCLC Proliferation
2
ARPE-19 Cell Proliferation Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The ARPE-19 cells were harvested and seeded into the 96-well plates at the density of 2 × 103 per well. The high-glucose (50mM) were then incubated with the cells for 0h, 12h, 24h and 36h, respectively. The commercial CCK-8 kit (AbMole, USA) was employed to measure cell proliferation according to the manufacturer’s protocol. Briefly, 10 μl of CCK-8 solution was added into each well for 4 h. After that, the plates were gently mixed and the Gemini EM microplate reader (Molecular Devices, USA) was used to measure the optical density (OD) values at the absorbance of 450 nm. The OD values were used to reflect the proliferation abilities of ARPE-19 cells.
3
Cell Proliferation Measurement Using CCK-8
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell growth was measured using a CCK-8 kit (AbMole, Shanghai, China). The cells were collected and seeded at 2000 cells/well in a 96-well plate, and the reaction solution was added and incubated at 37°C for 4 h. Cell proliferation was detected at 48 hours. The optical density was measured at an absorbance value of 450 nm.
4
Cell Proliferation Assay Using CCK-8
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CCK-8 kit (Abmole Bioscience, Houston) was adopted to detect cell proliferation in conformity to the product manual. After treatment or transfection, these cells were inoculated into a 96-well plate and incubated at 37°C. Subsequently, the OD450 value for each well was monitored by the use of a microplate reader (Dynex Technologies, West Sussex, UK) after the addition of 10 μl CCK-8 solution.
5
Evaluating 3T3-L1 Adipocyte Proliferation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The 3T3-L1 adipocytes were planked in 96-well plates at 5 × 103 cells/well in 100 μL of growth medium. The CCK-8 kit (AbMole, Shanghai, China) was used to detect cell proliferation according to the manufacturer’s instructions at 12, 24, 48 and 72 h after treatment with siRNA2-KDM2A.
6
Evaluating Cell Proliferation and Apoptosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The cell proliferation was assessed by the CCK-8 kit (AbMole, Houston, TX). Medium was mixed with CCK-8 solution, incubated with cells for 2 hours, then transferred to a new 96-well plate. OD 450 of each well was read by the Microplate Reader to manifest the cell number.
The percentage of apoptotic cells was detected by Annexin V-FITC/PI Apoptosis Detection Kit (Vazyme, Nanjing, China). Cells were collected and stained with Annexin V-FITC and PI sequentially. After that, cells were subjected to flow cytometry analysis and the data were analyzed with FlowJo software. Cells were distributed in 4 phases according to the PI and FITC status. FITC+/PI+ and FITC+/PI- cells were the apoptotic cells.
The percentage of apoptotic cells was detected by Annexin V-FITC/PI Apoptosis Detection Kit (Vazyme, Nanjing, China). Cells were collected and stained with Annexin V-FITC and PI sequentially. After that, cells were subjected to flow cytometry analysis and the data were analyzed with FlowJo software. Cells were distributed in 4 phases according to the PI and FITC status. FITC+/PI+ and FITC+/PI- cells were the apoptotic cells.
7
Bufalin Cytotoxicity Assay in PLC5 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell viability was detected using CCK-8 kit (AbMole). In brief, cells (2 × 103 /well) were seeded in the 96-well plate covered with 10% FBS-containing DMEM media and cultured overnight. Various concentrations of bufalin were incubated with cells for 48 hours or 10 nmol/L bufalin was incubated with cells for consecutive 5 days. Cells were then treated with CCK-8 reagent for 1 hour in the incubator. Optical density was measured using microplate reader (Thermo Scientific) in triplicate and the mean value of absorbance was referred to the quantity of viable cells. IC50 of bufalin in PLC5 cells for 48 hours was calculated using GraphPad Prism 5 (GraphPad Software, Inc.).
8
EdU Incorporation and Cell Viability Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
EdU (5-ethynyl-2′-deoxyuridine) incorporation assays were performed by using a kit (RIBOBIO, Guangzhou, China) following the instructions from the manufacturer. Briefly, cells were digested and transferred to 96-well plates at a density of 4 × 103–1 × 105 cells per well, then incubated with 50 mM EdU for 3 h and washed twice with PBS. After paraformaldehyde fixation, cells were neutralized with 2 mg/mL glycine solution, permeabilized with osmotic agent (0.5% PBS of Triton X-100), and stained with 1 × Apollo® dye solution at room temperature for 30 min. After washing twice with osmotic agent, cells were stained again 1 × Hoechst 33342 reaction solution for 30 min. Cells then were washed with PBS and imaged by fluorescence microscopy.
For cell counting, a cell counting kit-8 (CCK8 kit, Abmole Bioscience Inc., Houston, USA) was used for cell counting following the manufacturer's instructions. Cells were digested and transferred to 96-well plates at a density of 1000–4000/well. Each sample had two duplicates. Cells were incubated with CCK8 agent for 2 h and the signal (OD) was detected at 450 nm by spectrophotometry.
For cell counting, a cell counting kit-8 (CCK8 kit, Abmole Bioscience Inc., Houston, USA) was used for cell counting following the manufacturer's instructions. Cells were digested and transferred to 96-well plates at a density of 1000–4000/well. Each sample had two duplicates. Cells were incubated with CCK8 agent for 2 h and the signal (OD) was detected at 450 nm by spectrophotometry.
9
Transfection of 293T Cells with pEGFP-C1
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
293T cell lines and pEGFP-C1 plasmids were derived from our laboratory. Growth media (DMEM) was supplemented with 10% FBS and 1% antibiotic–antimycotic. 293T cell lines were maintained up to 30 passages in a 37 °C/5% CO2 incubator. All oligonucleotides were synthesized by Sangon (Shanghai, China). Trypsin EDTA and penicillin streptomycin were purchased from Biosharp (Hefei, China). DMEM basic media and fetal bovine serum and Opti-MEM were purchased from Gibco. Lipofectamine 3000 was purchased from Invitrogen. A CCK-8 kit was purchased from AbMole. A HiScribe™ T7 in vitro Transcription Kit and RNase H were purchased from NEB. A Total RNA Isolation Kit, HiScript III RT SuperMix for qPCR, and ChamQ Universal SYBR qPCR Master Mix were purchased from Vazyme (Nanjing, China).
10
Cell Proliferation Assay with CCK-8
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CCK-8 kit (AbMole, USA) was used to evaluate the proliferation of LGG cells according to the manufacturer’s protocol. LGG cells were plated into a 96-well plate with a concentration of around 1 × 103 per well. Then, cells were cultured at 5% CO2 and 37 °C for 24 h, 48 h, 72 h, 96 h, and 120 h respectively. Each well was then supplemented with 10 μL CCK-8 reaction solution followed by 2 h incubation. Then, the optical density (OD) values at 450 nm were recorded.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!