Golgi-cox staining was performed using the NovaUltra Golgi-Cox Stain Kit (IHCWorld, SKU IW-3023). Briefly, mice were deeply anesthetized and transcardially perfused with PBS. The mouse brains were immersed in the Golgi-Cox Solution in the dark at room temperature. After 2 days of immersion, fresh Golgi-Cox Solution was added to the samples, which were incubated at room temperature in the dark for an additional 14 days, according to the manufacturer’s instructions. After washing with PBS for two days, serial coronal sections (200-μm thick) were cut with a Vibratome Series 1000 Sectioning System. The coronal sections were washed with water and stained with Post-Impregnation Solution for 10 min in the dark at room temperature. Following three washes with water, the brain sections were mounted on Superfrost Plus slides (Thermo Scientific). The dendritic spine images were acquired using a 100× oil objective. Cortical pyramidal neurons were selected for analysis. The dendritic spines were counted in 50–100 μm segments that were at least 50 μm away from the cell body. The total spine density was measured using the NIH Image J plug-in simple neurite tracer.
Novaultra golgi cox stain kit
Manufactured by IHC World
Sourced in United States
The NovaUltraTM Golgi-Cox Stain Kit is a laboratory equipment product designed for the staining of neural tissue samples. The kit provides the necessary solutions and reagents to perform the Golgi-Cox staining technique, which allows for the visualization of the intricate dendritic and axonal structures of individual neurons within the tissue.
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5 protocols using novaultra golgi cox stain kit
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Golgi-Cox Staining for Dendritic Spine Analysis
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Golgi-Cox Analysis of MSN Dendrites in HD Mice
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Dendrite morphology of medium spiny neurons in wildtype and HD R6/2 mice was analyzed by Golgi-Cox staining using Novaultra Golgi-Cox Stain Kit (IHCWorld, SKU IW-3023). Briefly, mice were deeply anesthetized and transcardially perfused with 4% paraformaldehyde in PBS. After thoroughly washing with PBS overnight, mouse brains were immersed in the Golgi-Cox solution in dark at room temperature, then replaced with fresh Golgi-Cox Solution after 2 days of immersion and continued the impregnation at room temperature in dark for 14 days following the manufactory instruction. After washing with PBS for 2 days, serial coronal sections of 200 µm thick were cut on Vibratome Series 1000 Sectioning System. The coronal sections were washed with water and stained with Post-Impregnation Solution for 10 min in dark at room temperature. Following the 3 times of washing with water, the brain sections were mounted on Superfrost plus slides (Thermo Scientific). Medium spiny neurons in striatum were selected for analysis. Total dendritic length per neuron was measured using NIH Image J plug-in simple neurite tracer.
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Golgi-Cox Staining of Brain Tissue
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Golgi-Cox staining of brain tissue was performed using a NovaUltraTM Golgi-Cox Stain Kit (IHC World, Woodstock, MD, USA) according to the procedure suggested by the manufacturer. The protocols for Golgi staining, reconstruction, and morphometric analysis have been described in a previous publication by our group14 (link).
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Golgi-Cox Staining of Brain Tissue
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Golgi-Cox staining of brain tissue was performed using a NovaUltraTM Golgi-Cox Stain Kit (IHC World, Woodstock, MD, USA) according to the procedure suggested by the manufacturer. The protocols for Golgi staining, reconstruction, and morphometric analyses have been described in a previous study published by our group28 (link). On average, 6–8 neurons from each brain were selected based on the degree of preservation and visibility of the soma and dendritic trees.
5
Golgi-Cox Staining of Brain Tissue
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Golgi-Cox staining of brain tissue was conducted using a NovaUltraTM Golgi-Cox stain Kit (IHC World, Woodstock, MD, USA) according to the procedure suggested by the manufacturer. Briefly, 2 days after the last behavioral test, mice were anesthetized, decapitated, and the brain was rapidly removed. The fresh brain tissue was immediately immersed in a plastic jar filled with Golgi-Cox solution and stored in the dark at room temperature for 2 days. One day after immersion, the brain tissue was placed in fresh Golgi-Cox solution, and the impregnation was continued at room temperature in the dark for 14 days. When impregnation was finished, brain tissue was washed 2 times with phosphate-buffered saline (PBS) for 2 days, and then 100 µm thick sections were prepared using a vibratome (Leica, Wetzlar, Germany). The section was washed with distilled water (3 times, 5 min each) and stained with Post-Impregnation Solution for 10 min. Stained section was again washed with distilled water (3 times, 5 min each), mounted, and air-dried for 2 h. The slides were dehydrated in 70% and 95% ethanol for 5 min each, and then in 100% ethanol 2 times for 10 min each. The slides were then cleared with xylene 2 times for 10 min each and coverslipped with Permount. The slides were protected from light throughout the staining procedure and analysis.
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