Iron 3 chloride 6 hydrate

The following reagents and enzymes were used: Folin–Ciocalteau reagent, gallic acid, ABAP (n-hexane, 2,2′-azobis-2-amidinopropane), ABTS (2,2′-Azinobisbis(3-ethylbenzothiazoline-6sulfonic acid) diammonium salt), DPPH (2,2-diphenyl-1picrylhydrazyl), TPTZ (2,4,6-Tris(2-pyridyl)-s-triazine), Trolox (6-Hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), iron (III) chloride-6-hydrate (FeCl₃·6H₂O), potassium peroxodisulphate (K₂S₂O₈), and fluorescein were supplied by Sigma-Aldrich Co. α-amylase extracted from human saliva A1031, pepsin from porcine gastric mucosa P7000, pancreatin from porcine pancreas P1750, and bile salts B8756 were supplied by Sigma-Aldrich Co for the simulated digestion process. Other commonly used laboratory reagents of analytical grade were purchased from Merck KGaA (Darmstadt, Germany), Panreac (Barcelona, Spain), and Sigma-Aldrich Co. (St. Louis, MO, USA).

All reagents used were of analytical grade. Folin–Ciocalteu reagent, phenol solution, sodium carbonate (Na₂CO₃), 1,1-diphenyl-2-picrylhydrazyl (DPPH^•), 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ATBS), methanol and ethanol (HPLC grade), potassium ferricyanide (K₃[Fe(CN)₆]), potassium persulfate (K₂S₂O₈), potassium hydroxide (KOH), 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ), 2,4-dinitrophenylhydrazine (DNPH), guanidine HCl, iron(III) chloride 6-hydrate (FeCl₃^•6H₂O), iron(II) sulfate 7-hydrate (FeSO₄^•7H₂O), acetic acid (CH₃COOH), hydrochloric acid (HCl), sulfuric acid (H₂SO₄), sodium hydroxide (NaOH), aluminum chloride (AlCl₃), glucose, gallic acid, tetramethoxypropane (TMP), butylated hydroxytoluene (BHT), and rutin were purchased from Sigma Chemicals (St. Louis, MO, USA). Pancreatin, lipase enzymes, and Oxgall media were obtained from Merck (Darmstadt, Germany). Trichloroacetic (TCA) and 2-thiobarbituric acid (2-TBA) were supplied by JT Baker (Baker ^®, Phillipsburg, NJ, USA). POP was purchased from Aloha Medicinals, Inc.

Zinkuran SC was acquired from Arysta LifeScience Slovakia s.r.o. (Nové Zámky, Slovakia). Sodium selenate was purchased from Alfa Aesar (Karlsruhe, Germany). Nitric acid (HNO₃, for trace element analysis) was obtained from LGC Standards (Molsheim, France), and hydrogen peroxide (H₂O₂) 30% (Suprapur) was obtained from Merck/VWR (Leuven, Belgium). Methanol was purchased from Biosolve (Valkenswaard, the Netherlands). Acetone, sodium carbonate (Na₂CO₃), and sodium hydroxide (NaOH) were acquired from Merck (Darmstadt, Germany). Ascorbic acid (vitamin C), (+)-catechin, gallic acid, Folin–Ciocalteu's phenol reagent, 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) diammonium salt, 2–2′-azobis (2-methylpropionamidine) dichloride (AAPH), aluminum chloride (AlCl₃), and iron(II) sulfate were obtained from Sigma–Aldrich (St. Louis, MO, USA). Hydrogen chloride (HCl) and sodium nitrite (NaNO₂) were obtained from VWR (Leuven, Belgium). Iron(III) chloride 6-hydrate was purchased from Merck (Darmstadt, Germany).

The cellulase Cellusoft (EC 3.2.1.4) was kindly obtained from Novozymes A/S (Denmark), GA solution (25%), bovine serum albumin (BSA), CMC (carboxymethylcellulose), DNS (3,5-dinitrosalicylic acid, citrate buffer, and glucose standard were supplied by Sigma-Aldrich, Darmstadt, Germany). Ammonia solution (25%) was supplied by Chem-Lab (Belgium). Sodium cyanoborohydride (NaBH₃CN), trichloromethane, 1-propanol, acetonitryle, sodium dihydrogen phosphate (NaH₂PO₄), Coomassie brilliant blue, ortho-phosphoric acid (88% (v/v)), iron (II), chloride-4-hydrate (FeCl₂ × 4H₂O), iron (III) chloride-6-hydrate (FeCl₃ × 6H₂O), citric acid, and sodium chloride were purchased from Merck (Darmstadt, Germany). Methanol and acetone were supplied by Carlo Erba (Carnaredo, Italy). Ethanol was obtained from Kefo (Slovenia), glycerol was purchased from Kemika (Zagreb, Croatia), and 2-propanol, sodium silicate (Na₂SiO₃), and 3-aminopropyl-trimethoxysilan (APTMS) (97%) were obtained from Sigma-Aldrich (Darmstadt, Germany).