Nanoscan small animal pet ct scanner

This procedure was based on previous studies [9 (link), 81 , 82 (link)]. Rats were habituated to experimenter handling and the open field arena. Rats were fasted 16 h before the experiment. On the day of the experiment, rats received a continuous i.v. infusion of vehicle (buffered saline), (S)-ketamine (10 mg/kg), or HNK (10 mg/kg) over 40 min in an open field arena. Ten minutes after start of infusion, rats were injected (IP) with 13 MBq of 2-deoxy-2-[18F]-fluoro-D-glucose (FDG; Cardinal Health). After 30 min, rats were anesthetized with 1.5% isoflurane, placed on a custom-made bed of a nanoScan small animal PET/CT scanner (Mediso Medical Imaging Systems) and scanned for 20 min on a static acquisition protocol, followed by a CT scan. The PET data were reconstructed and coregistered to an MRI template using PMOD4.2 software environment (PMOD Technologies, Switzerland). The standardized uptake value ratio (SUVR_WB) images were calculated using the whole brain as a reference region. All statistical parametric mapping analyses were performed using Matlab R2016 (Mathworks) and SPM12 (University College London). The ROI-based data was obtained using PMOD and the Schiffer rat VOI-atlas[83 (link)] and the statistical analyses were performed using GraphPad’s Prism 9.

Rats were habituated to experimenter handling and the open field arena. On the day of the experiment, rats were injected (IV) with 13 MBq of [¹⁸F]Fallypride (Johns Hopkins University) right before receiving a continuous IV infusion of vehicle (buffered saline), (S)-ketamine (10 mg/kg), or (R)-ketamine (10 mg/kg) for 40 min in an open field arena. Rats were anesthetized with 1.5% isoflurane, placed on a custom-made bed of a nanoScan small animal PET/CT scanner (Mediso Medical Imaging Systems) and scanned for 30 min on a static acquisition protocol, followed by a CT scan. The PET data were reconstructed and coregistered to an MRI template as described above. Coregistered images were analyzed using one-way ANOVA and the resulting parametric images were filtered for statistically significant (p < 0.05) clusters larger than 100 contiguous voxels. All statistical parametric mapping analyses were performed using Matlab R2016 (Mathworks) and SPM12 (University College London).