Reference standards of apigenin, daidzein, daidzin, formononetin, genistein and genistin were obtained from Sigma-Aldrich Co. (St Louis, MO, USA); astragalin, calendoflavoside, cosmosiin, cynaroside, glycitin, hyperoside, isoquercitrin, isorhamnetin 3-O-glucoside, luteolin, narcissin, nicotiflorin and rutin as well as 6-methoxyluteolin and 6-methoxyflavone as internal standards were purchased from Extrasynthese (Genay, France); 6-O-malonyldaidzin and 6-O-malonylgenistin from Synthose Inc. (Ontario, Canada); kaempferol 3-O-gentiobioside, quercetin 3-O-gentiobioside and quercetin 3-O-sophoroside from PhytoLab GmbH & Co. (Vestenbergsgreuth, Germany); Cacticin and trifolin from MedChemExpress (Monmouth Junction, USA). LC–MS grade methanol, acetonitrile and water were supplied from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Besides, formic acid (Junsei Chemical, Tokyo, Japan) was used as eluent additive in extraction and chromatographic separation of flavonoid derivatives.
Narcissin
Manufactured by Extrasynthese
Sourced in France
Narcissin is a laboratory instrument used for the detection and quantification of narcotic substances. It is designed to provide accurate and reliable results for analytical and forensic applications.
Automatically generated - may contain errors
Lab products found in correlation
Nicotiflorin, by Extrasynthese (3 mentions)
Rutin, by Extrasynthese (3 mentions)
Methanol, by Merck Group (3 mentions)
Hyperoside, by Extrasynthese (2 mentions)
Isoquercitrin, by Extrasynthese (2 mentions)
Gallic acid, by Extrasynthese (2 mentions)
Acetonitrile, by Thermo Fisher Scientific (2 mentions)
Formic acid, by Merck Group (2 mentions)
Hplc grade acetonitrile, by Merck Group (2 mentions)
Daidzin, by Merck Group (1 mentions)
Formononetin, by Merck Group (1 mentions)
Glycitin, by Extrasynthese (1 mentions)
Formic acid, by Junsei (1 mentions)
Luteolin, by Extrasynthese (1 mentions)
Cynaroside, by Extrasynthese (1 mentions)
Apigenin, by Merck Group (1 mentions)
Genistein, by Merck Group (1 mentions)
Isorhamnetin 3 o glucoside, by Extrasynthese (1 mentions)
6 methoxyluteolin, by Extrasynthese (1 mentions)
6 methoxyflavone, by Extrasynthese (1 mentions)
5 protocols using narcissin
1
Comprehensive Flavonoid Characterization Protocol
2
Comprehensive Analytical Procedures for Phytochemical Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following chemicals were used: methylglyoxal (40% in water), 2,2–diphenyl–1–picrylhydrazyl,2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid, trolox, metformin hydrochloride, 98–100% formic acid, methanol (HPLC grade), acetonitrile (HPLC gradient grade and LC–MS grade), and water (LC–MS grade) were purchased from Merck–Sigma–Aldrich (Sigma–Aldrich Sp. z o.o., Poznań, Poland); NaCl, KCl, Na2HPO4 and KH2PO4 (reagent grade) were obtained from Chempur (Piekary Śląskie, Poland); quercetin, quercitrin, astragalin, hyperoside, rutin, nicotiflorin, narcissin, taxifolin, gallic acid, p-coumaric acid, ferulic acid, caffeic acid, and chlorogenic acid were from Extrasynthese (Genay Cedex, France); galegine sulfate was purchased from SelectLab (Münster, Germany). Water was glass-distilled and deionized.
The stock solutions of standards (1 mg/mL) were prepared by dissolving 5 mg of a reference compound in 5 mL of methanol. Working standard solutions in the range of 10–400 μg/mL were made by mixing with 50% aq. (aqueous) methanol (v/v), filtered through hydrophilic Millex Syringe Filters (Durapore 0.22 μm; Millipore, Burlington, MA, USA) and stored at −20 °C.
The stock solutions of standards (1 mg/mL) were prepared by dissolving 5 mg of a reference compound in 5 mL of methanol. Working standard solutions in the range of 10–400 μg/mL were made by mixing with 50% aq. (aqueous) methanol (v/v), filtered through hydrophilic Millex Syringe Filters (Durapore 0.22 μm; Millipore, Burlington, MA, USA) and stored at −20 °C.
3
Profiling Flavonoid Compounds in Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Reagents were purchased from various suppliers as follows: authentic standards of rutin, isoquercitrin, isorhamnetin, isorhanmetin 3-O-glucoside, narcissin, kaempferol (Extrasynthèse, Genay, France); FCR (Folin-Ciocalteu’s reagent), gallic acid, p-hydroxybenzoic acid, sodium carbonate, sodium hydroxide, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,2′-Azobis (2-methylpropionamidine) dihydrochloride (AAPH), fluorescein sodium salt and 2′,7′-dichlorofluorescein diacetate (DCFH-DA), as well as acetonitrile (HPLC grade), ethanol, methanol, formic acid (Sigma-Aldrich, St. Louis, MO, USA). In all experiments, Milli-Q (Merck Millipore, Darmstadt, Germany) water was used.
4
Enzymatic Activity of Plant-Derived Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
4-methylumbelliferone, hesperidin, rutin, hesperidin methylchalcone, isoquercetin, resorcinol, phloroglucinol, methanol, ethanol, butanol, n-propanol, isopropanol, isoamyl alcohol, 2-phenylethanol, p-Nitrophenyl-β-d -glucopyranoside and p-Nitrophenyl-α-l -rhamnopyranoside were purchased in Sigma Chemical (Sigma, St. Louis, MO, USA). Narcissin, tulipanin and myrtillin were purchased in Extrasynthese company (Extrasynthese, Genay Cedex, France). HPLC-grade methanol (LiChrosolv®) was obtained from Merck (Merck, Darmstadt, Germany). The 4-methylumbelliferyl-rutinoside reagent was synthesized as described in Mazzaferro et al. [29 (link)].
5
Detailed Protocol for OFI Fruit Extract Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All chemicals and reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless stated otherwise. Narcissin and nicotiflorin were purchase from Extrasynthese (Genay, France). Solvents for HPLC experiments were purchased from Burdick & Jackson (Morristown, NJ, USA). All cell culture reagents were purchased from Gibco (Grand Island, NY, USA) unless stated otherwise. The Lyophilized powder of dried OFI fruits was purchased from Jeju Cactus Town (Hallim, Jeju-do, Korea) and a representative sample (150101MC) has been deposited at the Herbarium of the College of Science, Sookmyung Women’s University, Seoul, Republic of Korea. The composition of the OFI fruit extract has been analyzed using HPLC-diode array detector (DAD) as described previously [12 (link),13 (link)] (Figure S1A and Table S3 ). Stock solutions of chemicals or OFI powder were prepared in dimethyl sulfoxide (DMSO) or olive oil and a solution of the lyophilized powder of OFI fruits was defined as OFIE in this study. The enzyme-hydrolyzed (hdl) OFIE sample was prepared as described previously [13 (link)]. The composition of the hdl OFIE has been presented in Figure S1B and Table S3 . Human liver microsomal fraction (UltraPoolTM HLM150; Catalog No. 452117) was purchased from Corning (San Jose, CA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!