Fei tecnai g2 spirit microscope

The electrochemical techniques such as differential pulse voltammetry (DPV), cyclic voltammetry (CV) and chronoamperometry (CA) were conducted using a Metrohm-Autolab potentiostat/galvanostat system (PGSTAT128N, Metrohm, Herisau, Sweden). Electrochemical impedance spectroscopy was performed under a 0.1 Hz to 100 kHz frequency using an IVIUM Compactstat (Eindhoven, The Netherlands) device. The employed screen-printed electrode had a Ag/AgCl paste and Pt electrodes on their own surface, as a reference and a counter electrode, respectively. UV–Vis was recorded using a double beam spectrophotometer (Shimadzu, Kyoto, Japan) model UV-1800 and quartz cells (Hellma, Müllheim, Germany). SEM and EDX were observed micrographs of the materials by ZEISS GeminiSEM 560 at 3.00 kV. The X-ray diffraction pattern was recorded using a Rigaku smart laboratory diffractometer (operated at 40 kV and 20 mA) with a Cu Kα source at a wavelength of 1.540 Å. TEM images were performed using an FEI Tecnai G2 Spirit microscope (Thermo Fisher Scientific, Waltham, MA, USA) at 120 kV. All electrochemical measurements were performed at 27.5 °C unless otherwise specified.

To determine phage morphology, purified high-titer phages in SM buffer were analyzed. Briefly, the glow discharge technique (30 s at 7.2 V using a Bal-Tec MED 020 coating system) was applied over carbon-coated copper grids, and grids were immediately placed on top of sample drops for 10 min. After two brief washes in distilled water, samples were contrasted with 2% uranyl acetate for 5 min. Excess fluid was removed and allowed to dry before examination with a transmission electron FEI Tecnai G2 Spirit microscope (Thermo Fisher Scientific, OR, USA). All images were acquired using Radius software (version 2.1) with a digital camera Xarosa (EMSIS, Münster, Germany).

TEM images were recorded with an FEI Tecnai G2 spirit microscope (Thermo Fisher Scientific, USA) operating at an accelerating voltage of 120 kV. For the characterization of the doped cells, after incubation with Zn_0.5Fe_2.5O₄/SiO₂ NPs, the human osteosarcoma MG-63 cells (1 × 10⁶) were washed three times with PBS and fixed with 1.5% glutaraldehyde in PBS at 4°C for 30 min. The fixed cells were washed three times with PBS and 1% osmium tetroxide in PBS was added for 1 h at room temperature. After another three washing steps in PBS, the cells were dehydrated with 30, 50, 75, 85, 95 and 100% (three times) absolute ethanol. Thereafter, the cells were infiltrated with Epon resin (two steps: 50 and 66% for resin in absolute ethanol, 30 min each) and embedded in 100% resin at 60°C for 2 days. Ultrathin sections (70 nm thick) were cut on an Ultramicrotome (Leika), stained with lead citrate and observed by TEM.