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Mtesrtm1 media

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MTeSR™1 is a complete and defined medium for the feeder-free culture of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). It contains all the necessary components for the maintenance and expansion of undifferentiated stem cells.

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Lab products found in correlation

Matrigel, by Corning (1 mentions) Matrigel, by BD (1 mentions)

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MTeSRTM1 (17 citations)

2 protocols using mtesrtm1 media

1

Human Embryonic Stem Cell Culturing

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Human embryonic stem cell line, HES‐3 (46 X, X) was obtained from ES Cell International (ESI, Singapore). The cells were cultured at 37°C in a humidified atmosphere with 5% CO2, on matrigel‐coated culture dishes (Falcon) with daily replacement of mTeSRTM1 media (Stemcell Technologies). Cells were passaged by mechanically cutting with cell cutters (Invitrogen) into small square cell sheets, scraping from the culture dish using cell scrapers and transferring to freshly matrigel‐coated culture dishes. Culture dishes were preincubated with matrigel (Corning) at 4°C overnight.
Tan H.L., Tan B.Z., Goh W.X., Cua S, & Choo A. (2019). In vivo surveillance and elimination of teratoma‐forming human embryonic stem cells with monoclonal antibody 2448 targeting annexin A2. Biotechnology and Bioengineering, 116(11), 2996-3005.
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2

Cardiomyocyte Differentiation from Human Embryonic Stem Cells

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Human embryonic stem cell (hESC) H1 line was obtained from WiCell Research and maintained on feeder-free plates pre-coated with Matrigel (BD Biosciences) using mTeSRTM1 media (STEMCELL Technologies Inc.). The cardiomyocyte differentiation was derived from hESCs. In short, dissociated hESCs were seeded on Matrigel pre-coated dishes in mTeSR media in the presence of ROCK inhibitor Y-27632 (Reagents Direct,Cat. 53-B85-50). A GSK inhibitor, CHIR (Selleck, Cat. S2924) was applied for 24 h, followed by addition of the canonical Wnt-signaling inhibitor, IWP4 (Stemgent, Cat. 04–0036). During differentiation, cells were cultured in RPMI medium plus B-27 Supplement.
Hernandez-Benitez R., Wang C., Shi L., Ouchi Y., Zhong C., Hishida T., Liao H.K., Magill E.A., Memczak S., Soligalla R.D., Fresia C., Hatanaka F., Lamas V., Guillen I., Sahu S., Yamamoto M., Shao Y., Aguirre-Vazquez A., Nuñez Delicado E., Guillen P., Rodriguez Esteban C., Qu J., Reddy P., Horvath S., Liu G.H., Magistretti P, & Izpisua Belmonte J.C. (2024). Intervention with metabolites emulating endogenous cell transitions accelerates muscle regeneration in young and aged mice. Cell Reports Medicine, 5(3), 101449.
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