2 × 106 freshly isolated mouse splenocytes, resuspended in PBS with 2% FBS, were labeled for 30 min at 4°C in the dark with different antibody panels. The antibodies used were: α-CD8 (5H10, Thermo Fisher, cat. nr. MCD0828TR), α-CD4 (RM4-5, Thermo Fisher, cat. nr. 56-0042-80), α-CD3 (17A2, eBioscience, cat. nr. 46-0032-80), α-CD62L (MEL-14, Biolegend, cat. nr. 104411), α-CD44 (IM7, Biolegend, cat. nr. 103005), α-PD-1 (29F-1A12, Biolegend, cat. nr. 135223), α-IL7R (A7R34, Biolegend, cat. nr. 135031), α-CTLA-4 (UC10-4B9, eBioscience, cat. nr. 12-1522-81), α-B220 (RA3-6B2, eBioscience, cat. nr. 47-0452-82), α-CD21 (eBio4E3, eBioscience, cat. nr. 48-0212-80), α-CD23 (B3B4, Biolegend, cat. nr. 101612), α-CD86 (GL1, eBioscience, cat. nr. 12-0862-81), α-CD69 (H1.2F3, eBioscience, cat. nr. 11-0691-81), α-IAb (AF6-120.1, eBioscience, cat. nr. 46-5320-80). The cells were then washed twice with PBS, resuspended in PBS with 2% FBS and data were acquired on an LSRII Flow Cytometer (BD Biosciences) and analyzed with FlowJo software, ver. 9.9.6 (Treestar, Inc).
α cd8 5h10
Manufactured by Thermo Fisher Scientific
The α-CD8 (5H10) is a monoclonal antibody that specifically binds to the CD8 antigen, which is expressed on the surface of a subset of T lymphocytes. This antibody can be used for the identification and enumeration of CD8-positive cells in flow cytometry and other immunoassay applications.
Automatically generated - may contain errors
Lab products found in correlation
Mel 14, by BioLegend (1 mentions)
Ra3 6b2, by Thermo Fisher Scientific (1 mentions)
Rm4 5, by Thermo Fisher Scientific (1 mentions)
Uc10 4b9, by Thermo Fisher Scientific (1 mentions)
Af6 120, by Thermo Fisher Scientific (1 mentions)
Lsrii flow cytometer, by BD (1 mentions)
Thapsigargin, by Thermo Fisher Scientific (1 mentions)
Fluo 4, by Thermo Fisher Scientific (1 mentions)
Fura red, by Thermo Fisher Scientific (1 mentions)
2 protocols using α cd8 5h10
1
Comprehensive Phenotyping of Mouse Splenocytes
2
Calcium Flux in Mouse Splenocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
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4 × 106 freshly isolated mouse splenocytes, resuspended in HBSS with 2% FBS, were labeled with 1 μM Fluo-4 and 2 μM Fura Red (Thermo Fisher, cat. nr. F14201, F3021) for 45 min at room temperature in the dark. Cells were then washed, stained with α-B220 (RA3-6B2, eBioscience, cat. nr. 47-0452-82), α-CD8 (5H10, Thermo Fisher, cat. nr. MCD0828TR), and α-CD4 (RM4-5, Thermo Fisher, cat. nr. 56-0042-80) for 30 min on ice, washed again and resuspended in HBSS with 2% FBS. After prewarming the cells for 15 min at 37°C, baseline Ca2+ levels were acquired for 30s. 1 μM of thapsigargin (Thermo Fisher, cat. nr. T7458) was then added to the cells and acquisition was continued for a total of 3 min. Data were acquired on an LSRII (BD Biosciences) and analyzed with FlowJo software (Treestar, Inc).
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