Uas flp

dpp-Gal4 [12 (link)], ptc-Gal4 [13 (link)], ap-Gal4 [14 (link)], Ubx-Gal4^M1 [15 (link)], col-Gal4 [16 (link)], UAS-omb [6 (link)], tub-Gal80^ts, UAS-GFP, UAS-RFP and UAS-FLP (Bloomington Stock Center), omb²⁸² [17 (link)], Df(2L)32FP5 [18 (link)] (a deficiency that removed the genes sal and salr), tkv^a12 [19 (link)]. The Df(2L)32FP5 tkv^a12 chromosome was generated by meiotic recombination. omb^P1-lacZ [20 (link)], 5XQE-DsRed [21 (link)] (a reporter of vgQE), sal-lacZ [22 (link)], puc^E69-lacZ [23 (link)], iro^rF209-lacZ [24 (link)], dally-lacZ [25 (link)], brk^X47-lacZ [26 (link)]. Dp(3;3)P5 is a tandem duplication of the bithorax complex [27 (link)].

The fly strains used: bam^Δ86[49] (link), bam^BG[50] (link), bam^BW[40] (link), bam-Gal4[51] (link), bamP-GFP[51] (link), UASp-bam-GFP[51] (link), bamP-bam-HA;bgcnP-bgcn-GFP[32] (link), bgcn^QS2[40] (link), bgcn²⁰⁰⁹³[52] (link), UAS-dcr2 (gifts from T.Tabata), Zip-GFP[53] (link), [54] (link), dj-GFP[36] (link), [55] (link), Hrb98DE-GFP[35] (link), [36] (link). nos-Gal4 and UAS-Flp were ordered from Bloomington Drosophila Stock Center; UAS-tutRNAi (v26044) was ordered from Vienna Drosophila RNAi Center. bgcn² was generated in our lab and contains the deletion of TGACG in the 2^nd intron of the gene.
Fly stocks were maintained under standard culture conditions and all flies were dissected 0–2 days after eclosure unless otherwise indicated. For RNAi experiments, flies were cultured at 25°C for 6 days and transferred to 29°C for another 6 days before dissection. For germline clonal analysis, flies were heat-shocked in 37°C water bath for 1 hour at late pupal stage and dissected 4–5 days after clone induction. tut⁴ homozygous and bam heterozygous phenotype varies at different temperatures, age, or nutritions. For tut⁴, bgcn², and bam/+ related experiments, flies were cultured at 24°C, fed with fresh yeast daily, and dissected within 12 hours after eclosure.

UAS-GFP-wg[27] (link), UAS-DFz2 and UAS-DFz2N[28] (link), UAS-Dlp-HA[16] (link), wg{KO; Gal4}[29] (link), wg{KO; wg-HA}[30] (link), wg^cx4, sfl⁰³⁸⁴⁴, Df(3R)Exel6193, Df(3R)BSC527, Df(3R)BSC619, Mi{ET1}CG13830^MB00767, neur-lacZ (neur^A101), UAS-Shi^ts, UAS-lacZ, UAS-myrRFP, UAS-FLP, tub-p-Gal4, ap-Gal4, en-Gal4, hh-Gal4, ptc-Gal4, MS1096-Gal4, and nub-Gal4 were obtained from the Bloomington Drosophila Stock Center. The cow^5Δ allele was generated by imprecise excision of the Minos transposable element Mi{ET1}CG13830^MB00767 from the 3′-UTR of cow (see Figure S1A).

The Drosophila stocks used were hh-Gal4 (Tanimoto et al., 2000 (link)); ap-Gal4 (Calleja et al., 1996 (link)); ET-40 (named in the text as Psc-QF) and QUAS-mtdTomato (Potter et al., 2010 (link)); dpp-LHG-86Fb (Yagi et al., 2010 (link)); lexO-hid (a kind gift of Ainhoa Pérez-Garijo and Hermann Steller); puc-lacZ line (pucE69) and UAS-puc14C line (Martin-Blanco et al., 1998 (link)); act-Gal4, UAS-GFP, UAS-hid7 (named in the text as UAS-hid), UAS-Flp, UAS-LacZ, tub-Gal80^TS, arm-LacZ FRT80B and ubi-GFP FRT80B (Bloomington Drosophila Stock Center); UAS-en (Guillen et al., 1995 (link)); hs-Flp112 and act>stop>lacZ (Struhl and Basler, 1993 (link)); ubiP63E>stop>GFP (Evans et al., 2009 (link)); tubP>stop>Gal80 (Bohm et al., 2010 (link)); mew-YFP (named in the text as PS1α-YFP, Kyoto Stock Center CPTI-001678); Pc³ (Lewis, 1978 (link)); trx^E2 (Kennison and Tamkun, 1988 (link)). In the experiments using the Gal4/UAS system the general rule was to use only two UAS transgenes. This was to avoid the possibility of titrating the amount of Gal4 protein available for the UAS vectors.

Drosophila (RRID:FlyBase_FBst1021211) were reared on standard cornmeal/agar medium supplemented with yeast under 12 hr LD cycles at 25°C. Elav-GAL4 (C155) was obtained from Bloomington Stock Center. UAS-ChR2-XXL was a kind gift from Kittel Lab. UAS-dTrpA1was from Dr. Paul Garrity. TH-GAL4 was described in (Guo et al., 2014 (link)). Pdf-GAL4 was described in (Stoleru et al., 2004 (link)). UAS-mCD8GFP;Pdf-GAL4 was described in (Nagoshi et al., 2010 (link)). Flies expressing both GAL4 and UAS-dTrpA1 were reared at 18°C until tested. UAS-FLP (#55804) was obtained from Bloomington Stock Center.

Flies (Drosophila melanogaster) were generally kept at 25°C and subjected to a 12 h light – 12 h dark cycle. A cornmeal medium supplemented with yeast, fructose and molasses was used to rear the flies. Canton-S was used as wild-type (courtesy of R. Stocker). c739-Gal4 was obtained from Hiromu Tanimoto (Tohoku University) and OK107-Gal4 (106098) was received from Kyoto stock center. nSyb-Gal4 (51635), c305a-Gal4 (30829), 5-HTR1B-Gal4 (27636), GMR58E02-Gal4 (41347), G0239-Gal4 (12639), tubGal80^ts (7019), nos-Cas9 (54591) and UAS-FLP (55804, 55806) were obtained from Bloomington stock center.