Protein levels of inflammasome signaling proteins were determined in the cytosolic and mitochondrial fractions as described in [7 (link)]. Briefly, protein lysates were resolved in 10–20% Criterion TGX Stain-Free precasted gels (Bio-Rad), using antibodies (1:1000 dilution) to NLRC4 (Novus Biologicals, cat# NBP2–41124), caspase-1 (Novus Biologicals, cat# NB100–56565), caspase-11 (Novus Biologicals, cat# MAB8648), ASC (Santa Cruz, cat# sc-271054), IL-1β (Cell Signaling, cat# 12242S), IL-18 (Abcam, cat# ab71495), HSP60 (Cell Signaling, cat#12165) and β-actin (Sigma Aldric, cat# A5441). Quantification of band densities was done using the UN-SCAN-IT gel 6.3 Software (Silk Scientific Corporation). Chemilluminescence substrate (LumiGlo, Cell Signaling) was imaged with the ChemiDoc Touch Imaging System (BioRad).
Criterion tgx stain free precasted gels
Manufactured by Bio-Rad
Sourced in United States
The Criterion TGX Stain-Free precast gels are a type of laboratory equipment designed for protein electrophoresis. They are pre-made gels that allow for the separation and visualization of proteins based on their molecular weight. The gels are pre-stained with a compound that becomes fluorescent when exposed to ultraviolet (UV) light, enabling the detection of proteins without the need for additional staining steps.
Automatically generated - may contain errors
Lab products found in correlation
Chemidoc touch imaging system, by Bio-Rad (4 mentions)
Il 1β, by Cell Signaling Technology (4 mentions)
Caspase 1, by Novus Biologicals (4 mentions)
Un scan it gel 6, by Silk Scientific (3 mentions)
Il 18, by Abcam (2 mentions)
Lumiglo, by Cell Signaling Technology (2 mentions)
Caspase 11, by Novus Biologicals (2 mentions)
Hsp60, by Cell Signaling Technology (1 mentions)
β actin, by Merck Group (1 mentions)
Caspase 8, by Novus Biologicals (1 mentions)
Nlrp1, by Novus Biologicals (1 mentions)
Nlrp3, by Novus Biologicals (1 mentions)
Un scan it gel 5, by Silk Scientific (1 mentions)
4 protocols using criterion tgx stain free precasted gels
1
Inflammasome Protein Detection Protocol
2
Inflammasome Protein Expression Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Analyses of inflammasome protein expression were measured by immunoblot analysis as described in Raval et al. (2019) (link). Briefly, cortical lysates were resolved in 4–20% Criterion TGX Stain-Free precasted gels (Bio-Rad, Hercules, CA, United States), using antibodies (1:1,000 dilution) to NLRP1 (Novus Biologicals, Littleton, CO, United States), caspase-1 (Novus Biologicals, Littleton, CO, United States), ASC (Santa Cruz, Dallas, TX, United States), IL-1β (Cell Signaling Technology, Danvers, MA, United States), caspase-8 (Novus Biologicals, Littleton, CO, United States), caspase-11 (Novus Biologicals, Littleton, CO, United States), and β-actin (Sigma-Aldrich, St. Louis, MO, United States). Quantification of band densities was done using the UN-SCAN-IT gel 6.3 Software (Silk Scientific Inc., Orem, UT, United States) and membranes were imaged using the ChemiDoc Touch Imaging System (Bio-Rad, Hercules, CA, United States) following chemiluminescence.
3
Inflammasome Signaling Proteins in Extracellular Vesicles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For detection of inflammasome signaling proteins in isolated EV, EV were resuspended in protein lysis buffer and resolved by immunoblotting as previously described (de Rivero Vaccari et al., 2015 (link)). Briefly, following lysis of the pellet, proteins were resolved in 10–20% Criterion TGX Stain-Free precasted gels (Bio-Rad), using antibodies (1:1,000 dilution) to NLRP3 (Novus Biologicals, cat# IMG-6763A), caspase-1 (Novus Biologicals, cat# NB100-56565), ASC (Santa Cruz, cat# sc-271054), IL-1β (Cell Signaling, cat# 12242S), IL-18 (Abcam, cat# ab71495), CD81 (Life Technologies, cat# MA5-13548) and NCAM (Sigma, cat# C9672). Quantification of band density was done using the UN-SCAN-IT gel 5.3 Software (Silk Scientific Corporation). Ten microliters of sample was loaded. Chemilluminescence substrate (LumiGlo, Cell Signaling) in membranes was imaged using the ChemiDoc Touch Imaging System (BioRad).
4
Inflammasome Protein Expression Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Analyses of inflammasome protein expression were measured by immunoblot analysis, as described in [28 (link)]. Briefly, cortical lysates were resolved in 4–20% Criterion TGX Stain-Free precasted gels (Bio-Rad, Hercules, CA, USA), using antibodies (1:1000 dilution) to caspase-1 (Novus Biologicals, Centennial, CO, USA), ASC (Santa Cruz, Dallas, TX, USA), IL-1β (Cell Signaling, Danvers, MA, USA), and β-actin (Sigma Aldric, St Louis, MS, USA). Membranes were imaged using the ChemiDoc Touch Imaging System (Bio-Rad) following chemiluminescence. Quantification of band densities was performed using the UN-SCAN-IT gel 6.3 software (Silk Scientific Corporation, Orem, UT, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!