Immunofluorescence staining was performed on tissue and cell samples. For tissue samples, slides were deparaffinized, rehydrated, and treated with sodium citrate buffer for antigen retrieval. Slides were incubated with 3% goat serum for 30 min followed by primary antibodies. Cell samples were fixed in poly‐l ‐lysine polylysine (P2100, Solarbio, China), followed by permeabilization and non‐specific binding site blocking. Samples were treated at 4°C overnight with the following primary antibodies: rabbit anti‐MPO antibody, mouse anti‐NE antibody, goat anti‐shp2 antibody (ab9214, Abcam, USA), rabbit anti‐pad4 antibody (ab214810, Abcam, USA), and rabbit anti‐histone H3 (citrulline R2) antibody (ab174992, Abcam, USA). The next day, Samples were incubated with an Alexa fluorescein‐labeled secondary antibody for 2 h. DAPI (C1002, Beyotime, China) was used to stain cell nuclei. Then, samples were imaged by an inverted confocal microscope (LSM880 with airyscan, Carl Zeiss, Germany).
Ab174992
Manufactured by Abcam
Sourced in United States
Ab174992 is a polyclonal antibody that targets the protein Histone H3. It is intended for use in various research applications, such as Western blotting, immunohistochemistry, and immunocytochemistry.
Automatically generated - may contain errors
2 protocols using ab174992
1
Immunofluorescence Staining of Tissue and Cells
2
Immunofluorescence Staining of Immune Cells
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Immunofluorescence staining was performed on tissue and cell samples. For tissue samples, slides were deparaffinized, rehydrated, and treated with sodium citrate buffer for antigen retrieval. Slides were incubated with 3% goat serum for 30 min followed by primary antibodies. Cell samples were fixed in poly-L-lysine polylysine (P2100, Solarbio, China), followed by permeabilization and nonspecific binding sites blocking.
Samples were treated at 4 °C overnight with the following primary antibodies: rabbit anti-myeloperoxidase antibody (ab208670, Abcam, USA), mouse anti-neutrophil elastase antibody (sc-55549, Santa Cruz Biotechnology, USA), goat anti-shp2 antibady (ab9214, Abcam, USA), rabbit anti-pad4 antibody (ab214810, Abcam, USA), rabbit anti-histone H3 (citrulline R2) antibody (ab174992, Abcam, USA). Next day, Samples were incubated with an Alexa fluorescein-labeled secondary antibody for 2 h. DAPI (C1002, Beyotime, China) was used to stain cell nuclei. Then samples were imaged by an inverted confocal microscope (LSM880 with airyscan, Carl Zeiss, Germany).
Samples were treated at 4 °C overnight with the following primary antibodies: rabbit anti-myeloperoxidase antibody (ab208670, Abcam, USA), mouse anti-neutrophil elastase antibody (sc-55549, Santa Cruz Biotechnology, USA), goat anti-shp2 antibady (ab9214, Abcam, USA), rabbit anti-pad4 antibody (ab214810, Abcam, USA), rabbit anti-histone H3 (citrulline R2) antibody (ab174992, Abcam, USA). Next day, Samples were incubated with an Alexa fluorescein-labeled secondary antibody for 2 h. DAPI (C1002, Beyotime, China) was used to stain cell nuclei. Then samples were imaged by an inverted confocal microscope (LSM880 with airyscan, Carl Zeiss, Germany).
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