Sars cov 2 igg assay scovg

Measurement of SARS-CoV-2 anti-RBD (receptor binding domain) of spike (S) protein, the IgG-type antibodies was performed as described in detail previously by our group (18 (link), 19 (link)). Briefly, quantitative measurement of neutralizing anti-RDB specific IgG-type antibody levels was performed with the Siemens Advia Centaur XPT system using the Siemens Healthineers SARS-CoV-2 IgG assay (sCOVG) (Siemens Healthineers, Munich, Germany). Irsara et al. (20 (link)), showed a proper correlation (r = 0.84) of the positive sCOVG assay results with virus neutralization capacity. Measured index values were converted into WHO 20/136 approved international units of 1000 Binding Antibody Unit per milliliter (BAU/mL) using the following equation: (sCOVG index) × 21.8 = BAU/mL, where the diagnostic cut-off value was 21.8 BAU/mL), assay sensitivity was 10.9 BAU/mL (21 (link)).

Measurement of SARS-CoV-2 anti-Spike (S) IgG antibodies was performed as described in detail previously [12 (link)]. Briefly, quantitative measurement of neutralizing anti-RDB specific IgG-type antibody titers was performed with the Siemens Advia Centaur XPT system using the Siemens Healthineers SARS-CoV-2 IgG assay (sCOVG) (Siemens Healthineers, Munich, Germany). Irsara et al., showed a proper correlation (r = 0.84) of the positive sCOVG assay results with virus neutralization capacity [38 (link)]. Measured Index Values were converted into WHO 20/136 approved international units of 1000 Binding Antibody Unit per milliliter (BAU/mL) using the following equation: (sCOVG Index) × 21.8 = BAU/mL, where the diagnostic cut-off value was 21.8 BAU/mL), assay sensitivity was 10.9 BAU/mL [12 (link),39 (link)].