Film-forming solutions (FFS) were prepared by dissolving HPMC (6% w/v) and κ-carrageenan (0.7% w/v) separately in dH2O. The solutions were mixed (1:1) for 40 min at 65 °C using a heating magnetic stirrer (Fisher Bio-block scientific). To obtain non-active (control packaging) and active food packaging (AP), selected amounts of glycerol (25% w/w) and nisin (1 mg mL−1) were added during stirring. For the nano-active packaging (NAP), void and nisin-loaded rhamnosomes solutions were added separately to double concentrated FFS of HPMC:CR (H-C) blend to obtain a final concentration of 6% for HPMC and 0.7% CR. Finally, NCP films were obtained by pouring approximately 8 g FFS in the Petri-dishes (NEST Biotechnology) and left to dry at room temperature (25 °C) and relative humidity (∼50%) for 24–48 h. Dried packaging films were stored in airtight plastic jars with silica beads for further characterization.
Petri dishes
Manufactured by NEST Biotechnology
Sourced in United States
Petri dishes are circular, shallow containers used for the culture and growth of microorganisms in laboratories. They are typically made of transparent plastic or glass, providing a sterile environment for the cultivation of bacteria, fungi, and other microscopic organisms.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using petri dishes
1
Development of Active Food Packaging Films
2
Culturing Human Neuroblastoma SH-SY5Y Cells
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Human neuroblastoma SH-SY5Y cells (China Cell Resource Confederation, Beijing, China) were maintained in DMEM (High D-glucose) (Gibco/Invitrogen, Grand Island, NY, USA) supplemented with 10% (v/v) fetal bovine serum (FBS; PAA Laboratories, Pasching, Austria), 100 unit/mL penicillin and 100 μg/mL streptomycin (Gibco/Invitrogen, USA) as monolayer in petri dishes (NEST Biotechnology, Wuxi, Jiangsu, China) at 37°C and 5% CO2 with > 95% relative humidity as described previously (Mo et al., 2013 (link), 2016 (link)). Cells were passaged every two days. Cell numbers were counted using a Countess automated cell counter (Life technologies/Invitrogen, USA).
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