Masshunter workstation software package

Manufactured by Agilent Technologies

MassHunter Workstation software package is a comprehensive suite of tools designed for data acquisition, analysis, and reporting in mass spectrometry applications. It provides a unified interface to control and acquire data from various Agilent mass spectrometry instruments.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

MassHunter software (440 citations) MassHunter Workstation software (231 citations) MassHunter Workstation (97 citations) MassHunter software package (12 citations)

4 protocols using masshunter workstation software package

PAICS Enzyme Activity Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

A 0.20
mL solution containing 20 mM HEPES, pH 7.4, 100 mM NaCl, 10 mM MgCl₂, 50 mM sodium bicarbonate or sodium ¹³C-bicarbonate,
0.30 mM CAIR, 1 mM l-aspartate, 0.30 mM ATP, and PAICS proteins
(0 or 0.20 μM) was incubated at 25 °C. The reaction was
initiated by the addition of CAIR. At a desired time, a 10 μL
portion of the reaction solution was added to 190 μL of ice-cold
methanol and kept at 4 °C. A 10-μL portion of this methanol-diluted
solution was injected to a Phenomenex Luna HILIC column (Phenomenex
00B-4449-Y0; 3 μm beads, 200 angstrom pore size, 50 mm ×
3 mm) connected to an Agilent 1250 HPLC and an Agilent 6250 ESI-qTOF
mass spectrometer (flow rate 0.3 mL/min at 20 °C; solvent A:
acetonitrile with 10 mM triethylammonium bicarbonate, solvent B: water
with 10 mM triethylammonium bicarbonate). Negative ion mass spectra
were acquired using an Agilent 6545 quadrupole-time-of-flight (qTOF)
mass spectrometer and analyzed using Agilent MassHunter Workstation
software package. Mass spectral peak areas corresponding to M–H
ions were calculated, allowing up to 2 ppm errors in m/z values.

Shon H., Matveeva E.A., Jull E.C., Hu Y., Coupet T.A, & Lee Y.S. (2022). Evidence Supporting Substrate Channeling between Domains of Human PAICS: A Time-Course Analysis of 13C-Bicarbonate Incorporation. Biochemistry, 61(7), 575-582.

+ Open protocol

+ Expand

Metabolite Quantification by LC-MS/MS

Check if the same lab product or an alternative is used in the 5 most similar protocols

Samples were subjected to LC separation (Agilent 1290) on a C8 column (Zorbax SB-C8 Rapid Resolution HD, 2.1 × 100 mm, 1.8 µm, Agilent; column temperature: 20°C, injection volume: 1 µl). Separation was achieved by isocratic flow at 12% acetonitrile for 3.5 min followed by a gradient to 38% acetonitrile within 2.5 min. With an additional washing step (42% acetonitrile, 0.5 min) and re-equilibration to starting conditions, this resulted in a total cycle time of 7.5 min. All buffers contained 750 mg l⁻¹ octylammoniumacetate as ion pairing reagent. An online coupled triple quadrupole mass spectrometer (Agilent 6460) operating in SRM mode was used for quantification. Individual metabolites were identified by matching retention time and fragmentation pattern with commercially available standards. SRM transitions, ionization and fragmentation energies were optimized for each compound (electronic supplementary material, table S2). Ion source settings are listed in the electronic supplementary material, table S3. Data analysis was done in the Masshunter Workstation software package (Agilent). External calibration curves were measured repeatedly and used to determine absolute concentrations.

Grüning N.M., Du D., Keller M.A., Luisi B.F, & Ralser M. (2014). Inhibition of triosephosphate isomerase by phosphoenolpyruvate in the feedback-regulation of glycolysis. Open Biology, 4(3), 130232.

+ Open protocol

+ Expand

HPLC-MS/MS Analysis of Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

Analysis was conducted using an HPLC Agilent 1290 Infinity system interfaced with an Agilent 6460 Triple Quad LC/MS (QQQ), equipped with an electrospray ion source (ESI) operating in positive mode. The ESI configuration was: gas temperature 325 °C; gas flow rate 10 L/min; nebuliser 20 psi; capillary 4000 V. Chromatographic separation was performed through a Zorbax Eclipse Plus C18 (2.1 mm × 50 mm, 1.8 m, Agilent Technologies). The mobile phase initially consisted of 5 mM aqueous formic acid (A) and ACN (B) 99:1. Gradient of elution was carried out by increasing the % ACN to 30% within 6 min; to 50% within 2 min; to 100% within 4 min and isocratic for 3 min. The flow rate was 0.4 mL/min until 8 min, then increase at 0.6 mL/min within 2 min. Analysis was carried out first in scan mode (50–500 m/z) in positive and negative ionisation and then the collision-induced dissociations (CIDs) were studied at different collision energies (CE, 10, 20, 30 and 40 eV). Agilent MassHunter Workstation software package was used for data acquisition and elaboration.

Bertol E., Vaiano F., Mari F., Di Milia M.G., Bua S., Supuran C.T, & Carta F. (2017). Advances in new psychoactive substances identification: the U.R.I.To.N. Consortium. Journal of Enzyme Inhibition and Medicinal Chemistry, 32(1), 841-849.

+ Open protocol

+ Expand

GC-MS Analytical Procedure for Compound Identification

Check if the same lab product or an alternative is used in the 5 most similar protocols

The GC–MS instrument consisted in an Agilent 7890 A GC system equipped with an Agilent 7683B series autosampler and interfaced to a single quadrupole Agilent 5975C mass spectrometer. The column was an Agilent HP-5MS (30 m × 0.25 mm, 0.25 µm film thickness). The gas carrier (He) flow was constant at 1 mL/min. The samples were analysed in full scan mode (adopted libraries: NIST08, WILEY27, SWGDRUG4). The oven temperature was initially set at 100 °C for 2.25 min, programmed to 180 °C at 40 °C/min and to 300 °C at 10 °C/min for 10 min. Injector and transfer line temperatures were always 300 and 230 °C, respectively. The injection volume was 1 mL in splitless mode. Data acquisition and elaboration were performed using the Agilent MassHunter Workstation software package.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!