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App c terminal

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The APP C-terminal is a specialized laboratory equipment component designed for applications involving the amyloid precursor protein (APP). It is a critical part of the apparatus used to study the structure and function of the C-terminal region of the APP molecule. The core function of the APP C-terminal is to facilitate the analysis and investigation of this particular domain of the APP protein, which plays a crucial role in various biological processes.

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Lab products found in correlation

β actin, by Cell Signaling Technology (3 mentions) Synaptophysin, by Abcam (2 mentions) Psd95, by Cell Signaling Technology (2 mentions) Rbm25, by Abcam (2 mentions) Anti aβ42, by Merck Group (2 mentions) H2 ab1, by Santa Cruz Biotechnology (2 mentions) Staurosporine, by Merck Group (1 mentions) G 6976, by Bio-Techne (1 mentions) Mouse anti ha, by Merck Group (1 mentions) Phorbol 12 myristate 13 acetate, by Merck Group (1 mentions) P raptor, by Cell Signaling Technology (1 mentions) P ampk, by Cell Signaling Technology (1 mentions) P acc1, by Cell Signaling Technology (1 mentions) Sto 609, by Cayman Chemical (1 mentions) Bn1001, by Thermo Fisher Scientific (1 mentions) Atp5a, by Abcam (1 mentions) Raptor, by Cell Signaling Technology (1 mentions) Total gsk3β, by Cell Signaling Technology (1 mentions) Gsk3β ser9, by Cell Signaling Technology (1 mentions) Total s6k1, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

C-terminal, (4 citations) Anti-APP C-terminal antibody (3 citations) Anti-APP C-Terminal (3 citations) APP C-terminal antibody (A8717 (2 citations) C-terminal APP antibody (2 citations)

5 protocols using app c terminal

1

Antibody-Based Protein Trafficking Analysis

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Antibodies used were Mouse Anti-HA (Sigma, H9658), AP-3 (SA4, Developmental Studies Hybridoma Bank) and APP C-terminal (Sigma, A8717). The PKC activator Phorbol-12-myristate-13-acetate (PMA) was purchased from Sigma (P8139) and 8-[2-(2-pentyl-cyclopropylmethyl)-cyclopropyl]-octanoic acid (DCP-LA) was purchased from (Sigma, D5318). Staurosporine was purchased from Millipore (Cat No. 569397). Gö6976 was purchased from Tocris Bioscience (Cat. No. 2253).
Tam J.H., Cobb M.R., Seah C, & Pasternak S.H. (2016). Tyrosine Binding Protein Sites Regulate the Intracellular Trafficking and Processing of Amyloid Precursor Protein through a Novel Lysosome-Directed Pathway. PLoS ONE, 11(10), e0161445.
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2

Immunoblotting Analysis of Cellular Signaling

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Western blots were performed as previously described (Currais et al., 2014). Antibodies used were as follows: APP C‐terminal (Sigma, A8717), ATP5A (Abcam, 14748), Total Oxphos Rodent antibody cocktail (ab110413), pAMPK (Cell Signaling, 2535), AMPK (Cell Signaling, 2793), pS6 (Cell Signaling, 4858), S6 (Cell Signaling, 2317), praptor (Cell Signaling, 2083), raptor (Cell Signaling, 2280), pACC1 (Cell Signaling, 11818), ACC1 (Cell Signaling, 4190), and Actin (BD Transduction Laboratories™, 4125). Horseradish peroxidase‐conjugated secondary antibodies: goat anti‐rabbit, goat anti‐mouse (1:5,000, Bio‐Rad, 1706516, 1721019). The CamKK2 inhibitor STO‐609 (Cayman 15325) was used at 1 μg/ml. NativePAGE Western blots were performed according to manufacturer's protocol (ThermoFisher Scientific, BN1001).
Goldberg J., Currais A., Prior M., Fischer W., Chiruta C., Ratliff E., Daugherty D., Dargusch R., Finley K., Esparza‐Moltó P.B., Cuezva J.M., Maher P., Petrascheck M, & Schubert D. (2018). The mitochondrial ATP synthase is a shared drug target for aging and dementia. Aging Cell, 17(2), e12715.
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3

Antibody Panel for Neurodegenerative Markers

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The following antibodies were purchased from Abcam: BHMT (#ab96415), CD68 (#ab955), Rbm25 (#ab72237), Synaptophysin (#ab32127). Cell signaling: β-actin (#4967), Pin1 (#3722), PSD95 (#3409). Millipore: anti-Aβ42 (#5078P), 6E10 (#MAB1560), APP C-terminal (#171610). Santa Cruz Biotechnology: H2-Ab1 (#sc-71201). Wako: Iba1 (NCNP24).
Velazquez R., Ferreira E., Winslow W., Dave N., Piras I.S., Naymik M., Huentelman M.J., Tran A., Caccamo A, & Oddo S. (2019). Maternal choline supplementation ameliorates Alzheimer’s disease pathology by reducing brain homocysteine levels across multiple generations. Molecular psychiatry, 25(10), 2620-2629.
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4

Western Blot Antibody Validation Protocol

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The following antibodies were used for western blots: from Cell Signaling, Danvers, MA: total mTOR (1:1000; catalog number 2983S), total S6K1 (1:1000; catalog number 9202S), pS6K1 Thr389 (1:1000; catalog number 9205S), β-actin (1:10000; catalog number 3700S), total IRS-1 (1:1000; catalog number 3407S), IRS Ser318 (1:1000; catalog number 5610S), IRS Ser636/639 (1:1000; catalog number 2388S), totalPI3K p85 (1:1000; catalog number 4292S), PI3K Tyr458 (1:1000; catalog number 4228S), total AKT (1:1000 ; catalog number 9272), AKT Thr308 (1:1000; catalog number 2965S), AKT Ser473 (1:1000; catalog number3787S) total GSK3β (1:1000; catalog number 9315S), GSK3β Ser9 (1:1000; catalog number 9336S), CP13 (1:1000; catalog number 11834S). From Millipore, Billerica, MA: APP C-terminal (1:2000; catalog number 171610), Tau5 (1:5000; catalog number 577801). From BioLegend, San Diego, CA: APP (1:3000; catalog number MAB348). PHF-1 (1:3000) was a gift from Dr. Peter. Davies.
Caccamo A., Belfiore R, & Oddo S. (2018). Genetically reducing mTOR signaling rescues central insulin dysregulation in a mouse model of Alzheimer’s disease. Neurobiology of aging, 68, 59-67.
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5

Antibody Panel for Neurodegenerative Markers

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The following antibodies were purchased from Abcam: BHMT (#ab96415), CD68 (#ab955), Rbm25 (#ab72237), Synaptophysin (#ab32127). Cell signaling: β-actin (#4967), Pin1 (#3722), PSD95 (#3409). Millipore: anti-Aβ42 (#5078P), 6E10 (#MAB1560), APP C-terminal (#171610). Santa Cruz Biotechnology: H2-Ab1 (#sc-71201). Wako: Iba1 (NCNP24).
Velazquez R., Ferreira E., Winslow W., Dave N., Piras I.S., Naymik M., Huentelman M.J., Tran A., Caccamo A, & Oddo S. (2019). Maternal choline supplementation ameliorates Alzheimer’s disease pathology by reducing brain homocysteine levels across multiple generations. Molecular psychiatry, 25(10), 2620-2629.
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