PGZ was obtained from Capot Chemical (Hangzhou, People’s Republic of China) and Diblock copolymer PLGA-PEG (Evonik Ind., Resomer® Select 50:50 DLG mPEG 5,000–5 wt% PEG), was purchased from Evonik Corporation (Birmingham, AL, USA). Rhodamine 6G (Rhod), Tween® 80 (Tw 80), 70 kDA FITC-dextran and 24-well filter inserts (PET membrane, pore 1 µm) were acquired from Sigma-Aldrich (Gillingham, UK). The dialysis membrane MWCO 12,000–14,000 Da, was obtained from Medicell International Ltd. (London, UK). Trypsin-EDTA was purchased from Thermo Fisher (London, UK). Alamar Blue (AB) was obtained from Invitrogen Alf-agene® (Carcavelos, Portugal).
Tween 80 tw 80
Manufactured by Merck Group
Sourced in United States
Tween® 80 (Tw 80) is a non-ionic surfactant commonly used in the pharmaceutical and biotechnology industries. It is a polyoxyethylene sorbitan monooleate compound that acts as a wetting agent, emulsifier, and dispersant. Tween® 80 is soluble in water and is often used to improve the solubility and stability of various formulations.
Automatically generated - may contain errors
Lab products found in correlation
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Fitc dextran 70 kda, by Merck Group (1 mentions)
Resomer select 50 50 dlg mpeg 5 000 5 wt peg, by Evonik (1 mentions)
Pluronic f127 pf127, by Merck Group (1 mentions)
18 mer splice switching on, by GE Healthcare (1 mentions)
Tween 20 tw20, by Merck Group (1 mentions)
Polyvinylpyrrolidone pvp, by Merck Group (1 mentions)
Lipofectin, by Thermo Fisher Scientific (1 mentions)
Pluronic f 68 pf68, by Merck Group (1 mentions)
2 hydroxypropyl β cyclodextrin hpβcd, by Merck Group (1 mentions)
Thymoquinone tq, by Merck Group (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Formaldehyde, by Merck Group (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
Sodium selenite na2seo3, by Merck Group (1 mentions)
Paclitaxel ptx, by Tokyo Chemical Industry (1 mentions)
1 6 diphenyl 1 3 5 hexatriene dph, by Merck Group (1 mentions)
4 protocols using tween 80 tw 80
1
Nanoparticle-based Permeability Assay
2
Splice-switching ON and Gene Delivery Vectors
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The 18-mer splice-switching ON with the sequence (5′-CCUCUUACCUCAGUUACA) was synthesized at GE Healthcare. The ON had a fully modified phosphorothioate backbone and 2′-O-methyl modified ribose sugar units. 187.15-5LNA (5′-aGacTuaCcaCuuCc) was used for the BTK gene cell models [21 (link)]. The G2-RR peptide dendrimer (Figure 1 ) used in the study was synthesized using solid phase synthesis while applying Fmoc/Boc protection strategy, as reported previously [17 (link)]. Lipofectin (a liposomal formulation of a 1:1 (w/w) ratio of DOTMA (N-[1-(2,3-dioleyloxy)- propyl]-N,N,N-trimethylammonium chloride) and DOPE (dioleoyl phosphotidylethanolamine)), and Lipofectamine 2000 (L2000) were both purchased from Invitrogen (Stockholm, Sweden). Polyvinylpyrrolidone “PVP” (average molecular weights: 10, 40, and 55 kDa); Polyvinylalcohol “PVA” (average molecular weights: 18, and 40 kDa); Pluronic F 68 “PF68”; Pluronic F 127 “PF127”; Tween 80 “TW80”; Tween 20 “TW20”; (2-Hydroxypropyl)-β-cyclodextrin “HP-β-CD” and all other sugars were bought from Sigma–Aldrich (Stockholm, Sweden).
3
Thymoquinone-based Antioxidant Formulation
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Thymoquinone (TQ, Sigma-Aldrich,
USA), Tween 80 (TW-80), sodium selenite (Na2SeO3), ascorbic acid, formaldehyde, Triton X-100, and dimethyl sulfoxide
(DMSO) were purchased from Sigma-Aldrich (USA). Other reagents used
were of analytical grade.
USA), Tween 80 (TW-80), sodium selenite (Na2SeO3), ascorbic acid, formaldehyde, Triton X-100, and dimethyl sulfoxide
(DMSO) were purchased from Sigma-Aldrich (USA). Other reagents used
were of analytical grade.
4
Liposomal Paclitaxel Formulation
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1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) was purchased from the NOF AMERICA Corporation (White Plains, NY, US). Cholesterol, ethanol (≥99.5%, HPLC grade), Tween® 80 (TW80), and 1,6-diphenyl-1,3,5-hexatriene (DPH) were purchased from Sigma-Aldrich (St Louis, MO, US). Monohydrate, phosphate-buffered saline (PBS) was purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Paclitaxel (PTX) was purchased from Tokyo Chemical Industry (Tokyo, Japan).
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