Anti vdr

Anti-GFAP and anti-SOX2 antibodies were obtained from Dako, Agilent (Santa Clara, CA, United States), anti- VDR and anti-aSMase were from Elabscience (Houston, TX, United States) and anti-β tubulin was from Sigma Aldrich (St. Louis, MO, USA). Horseradish peroxidase-conjugated goat anti-rabbit secondary antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). TaqMan SNP Genotyping Assay and Reverse Transcription kit were purchased from Applied Biosystems (Foster City, CA, USA). RNAqueous^®-4PCR kit was from Ambion Inc. (Austin, Texas). SDS-PAGE molecular weight standards were purchased from Bio-Rad Laboratories (Hercules, CA, USA). Chemiluminescence kit was purchased from Amersham (Rainham, Essex, UK).

Cell culture medium (Dulbecco’s modified minimum essential medium, DMEM), fetal bovine serum (FBS), penicillin G, streptomycin, and glutamine, and sodium pyruvate were from GIBCO Invitrogen (Carlsbad, CA, USA). Anti-GFAP antibody was obtained from Dako, Agilent (Santa Clara, CA, USA), anti-VDR from Elabscience (Houston, TX, USA), anti-nSMase1 was from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA), anti-aSMase was from Abcam (Cambridge, UK), anti-β-tubulin and 1α,25(OH)₂VD3, 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2-tetrazoliumbromide (MTT) were from Sigma Aldrich (St. Louis, MO, USA), horseradish peroxidase-conjugated goat anti-rabbit secondary antibodies and tetramethylrhodamineisothiocyanate (TRITC)-conjugated anti-rabbit IgG were from Santa Cruz. TaqMan SNP Genotyping Assay and Reverse Transcription kit were purchased from Applied Biosystems (Foster City, CA, USA). RNAqueous^®-4PCR kit was from Ambion Inc. (Austin, TX, USA). SDS-PAGE molecular weight standards were purchased from Nzythech (Lisboa, Portugal). Chemiluminescence kitswerepurchasedfromAmersham (Rainham, Essex, UK).