The agarose pads with induced MG1665 cells were prepared similar to those in the “Imaging the IbpA protein with wide-field fluorescence microscopy ” section. SIM images were acquired on a Nikon N-SIM system equipped with a Nikon SR HP Apo TIRF 100X 1.49NA objective, a Hamamatsu ORCA-Flash4.0 camera (65 nm per pixel), and 488 nm and 561 nm lasers from a Nikon LU-NV laser launch. Cells were identified using DIC to avoid photobleaching. For each 2D-SIM image, nine images were acquired in different phases via the built-in 2D SIM modes. Super-resolution image reconstruction was performed using the Nikon Elements SIM module.
Sr hp apo tirf 100x 1.49na objective
Manufactured by Hamamatsu Photonics
The SR HP Apo TIRF 100X 1.49NA objective is a high numerical aperture (NA) objective lens designed for total internal reflection fluorescence (TIRF) microscopy applications. It provides a 100X magnification and a numerical aperture of 1.49, allowing for high-resolution imaging of samples near the coverslip surface.
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2 protocols using sr hp apo tirf 100x 1.49na objective
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Super-resolution Imaging of IbpA Protein
2
Super-resolution Imaging of IbpA Protein
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The agarose pads with induced MG1665 cells were prepared similar to those in the “Imaging the IbpA protein with wide-field fluorescence microscopy ” section. SIM images were acquired on a Nikon N-SIM system equipped with a Nikon SR HP Apo TIRF 100X 1.49NA objective, a Hamamatsu ORCA-Flash4.0 camera (65 nm per pixel), and 488 nm and 561 nm lasers from a Nikon LU-NV laser launch. Cells were identified using DIC to avoid photobleaching. For each 2D-SIM image, nine images were acquired in different phases via the built-in 2D SIM modes. Super-resolution image reconstruction was performed using the Nikon Elements SIM module.
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